1x1 image pixel for data collection
Pierce™ Albumin Serum Depletion Kits
Pierce™ Albumin Serum Depletion Kits
Thermo Scientific™

Pierce™ Albumin Serum Depletion Kits

Optimize serum sample prep by depleting unwanted albumin and IgG serum proteins for protein-profiling methods using quality depletion kits.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberDescription
85160Albumin Depletion Kit
89875Albumin and IgG Removal Kit
Catalog number 85160
Price (USD)
356.15
Special offer
Online exclusive
Ends: 15-Aug-2025
419.00
Save 62.85 (15%)
Each
In stock
Add to cart
Description:
Albumin Depletion Kit
Recurring order eligible. Learn more »
Request bulk or custom format
Price (USD)
356.15
Special offer
Online exclusive
Ends: 15-Aug-2025
419.00
Save 62.85 (15%)
Each
Add to cart
Ask our AI about this Product
Concentrations of albumin and IgG can make up more than 70% of total serum proteins and often mask lower quantity proteins during protein-profiling experiments. Optimize your serum sample prep by removing albumin or IgG quickly and conveniently. By using our depletion kits to remove excess high-abundance serum proteins, less abundant proteins of interest can be easily detected by 2D electrophoresis and other protein profiling methods such as mass spectrometry.
Pierce Albumin/IgG Removal Kit

Optimized—microcentrifuge spin cup method is scaled for treating 10 μL human serum samples for 2D electrophoresis
Efficient—removes nearly all HSA and IgG, enabling low-abundance proteins to be detected in 2D gels
Fast—processes samples in less than 40 minutes
Scalable—use different amounts of affinity resin to match sample volume and concentration

The Thermo Scientific Pierce Albumin/IgG Removal Kit uses a classical mixture of Cibacron Blue Dye and Protein A resin for removal of albumin and IgG. The dye binds to albumin with good specificity, and Protein A binds to many species and subclasses of IgG. The Pierce Albumin/IgG Removal Kit is economical and can be used to treat samples from other species besides human (e.g., monkey, swine, rabbit). The kit is also scalable and sufficient to process 25 samples, each containing 600 μg of total serum protein (about 10 μL of serum).

Pierce Albumin Depletion Kit

Complete—includes optimized buffer and microcentrifuge spin columns to remove albumin quickly and conveniently from 10- to 50-μL samples
Adaptable—easy-to-dispense slurry improves performance, eases processing of multiple samples, and can be adapted to larger or smaller columns or 96-well filter plates
Compatible—removing excess albumin facilitates MS analysis or electrophoresis gel analysis of low-abundance serum proteins

The Thermo Scientific Pierce Albumin Depletion Kit resin is supplied as a 50% slurry. To prepare the depletion columns, 200 μL of slurry is dispensed into the supplied microcentrifuge spin columns to obtain 100 μL of settled beads to process 10 to 50 μL of serum sample in a single reaction. The kit has been optimized to bind human, porcine, and sheep albumin from serum samples. With a modification to the protocol, albumin from bovine, calf, goat, and rat can be removed with this method. The kit is not effective for removal of mouse albumin. Processed samples are ready for immediate downstream analysis by electrophoresis or MS applications.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionAlbumin Depletion Kit
Final Product TypeProtein
For Use With (Equipment)Microcentrifuge, Mass Spectrometer
Quantity24 Reactions
Sufficient ForRemoving albumin from 24 human serum samples, 10 to 50 μL each
Workflow StepSample Enrichment, Sample Clean Up
Detection MethodMass Spectrometry
FormatKit
Product LinePierce™
Product TypeAlbumin Depletion Kit
Starting MaterialPlasma, Serum
Unit SizeEach
Contents & Storage
• Pierce Albumin Depletion Resin, 5 mL
• Binding/Wash Buffer, 11 mL
• Pierce Spin Columns, 24 columns

Store at 4°C.
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Figures

Customers who viewed this item also viewed



Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
3229455Certificate of AnalysisJul 03, 202589875
3215708Certificate of AnalysisJun 30, 202589875
3215112ACertificate of AnalysisJun 27, 202585160
3215112Certificate of AnalysisJun 27, 202585160
3214219Certificate of AnalysisJun 02, 202589875
5 results displayed, search above for a specific certificate

Safety Data Sheets

Other products to consider



Share catalog number, name or link