Search Thermo Fisher Scientific
Certificates
SDS
Gibco™
C57BL/6 Mouse Embryonic Fibroblasts, MitC-treated
Mitomycin C (MitC)-treated Gibco C57BL/6 Mouse Embryonic Fibroblasts (MEFs) are feeder cells that support the culture of healthy undifferentiated embryonicRead more
PromotionPromo code:RPUZZ25
Stock up on essentials to piece your discovery together
PromotionPromo code:RACCS25
Save on advanced culture solutions
| Catalog Number | Quantity |
|---|---|
| A34962 | 4 x 106 cells |
Catalog number A34962
Price (USD)
80.25
Each
In stock
Quantity:
4 x 106 cells
Price (USD)
80.25
Each
Mitomycin C (MitC)-treated Gibco C57BL/6 Mouse Embryonic Fibroblasts (MEFs) are feeder cells that support the culture of healthy undifferentiated embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), especially mouse ESCs/iPSCs. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6002M, these MEF cells are isolated from inbred C57BL/6 mice, mitotically arrested by MitC treatment, and tested to help ensure performance and minimize the risk of contamination. For our most widely used feeder cells for culturing pluripotent stem cells, see CF1 Mouse Embryonic Fibroblasts, Irradiated or CF1 Mouse Embryonic Fibroblasts, MitC-Treated. You may also choose from our selection of drug-resistant MEFs to suit your application.
• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Provide optimum support for mouse ESCs and iPSCs
Help save time with ready-to-use MEFs
MitC-treated C57BL/6 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.
Culture with meticulously tested feeder cells
Each lot of MitC-treated C57BL/6 MEFs is tested for bacteria, fungi, mycoplasma, and mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ESCs to help ensure robust and consistent performance. Culture your ESCs and iPSCs with confidence, knowing that you can rely on these high quality feeder cells.
Provide optimum support for mouse ESCs and iPSCs
While C57BL/6 MEFs can also support human ESCs and iPSCs, these feeder cells work particularly well for culturing mouse ESCs and iPSCs, showing better results compared to CF1 and ICR strain MEFs.
• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Provide optimum support for mouse ESCs and iPSCs
Help save time with ready-to-use MEFs
MitC-treated C57BL/6 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.
Culture with meticulously tested feeder cells
Each lot of MitC-treated C57BL/6 MEFs is tested for bacteria, fungi, mycoplasma, and mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ESCs to help ensure robust and consistent performance. Culture your ESCs and iPSCs with confidence, knowing that you can rely on these high quality feeder cells.
Provide optimum support for mouse ESCs and iPSCs
While C57BL/6 MEFs can also support human ESCs and iPSCs, these feeder cells work particularly well for culturing mouse ESCs and iPSCs, showing better results compared to CF1 and ICR strain MEFs.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Donor SourcePooled
No. of Cells4 x 106
Quantity4 x 106 cells
Shipping ConditionShipped on dry ice
AgeEmbryonic
Cell TypeFeeder Cells (Mouse Embryonic Fibroblast)
FormCryopreserved
SpeciesMouse
Unit SizeEach
Contents & Storage
Cryopreserved mouse embryonic fibroblasts
Store in liquid nitrogen.
Store in liquid nitrogen.
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our
Privacy Notice.
Figures
C57BL/6 Mouse Embryonic Fibroblasts, mitC-treated
Customers who viewed this item also viewed
Documents & Downloads
Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
2515283Certificate of AnalysisJan 29, 2025A34962
2937422Certificate of AnalysisJun 25, 2024A34962
2176876Certificate of AnalysisMar 01, 2020A34962
2053490Certificate of AnalysisFeb 25, 2019A34962
20010645Certificate of AnalysisJul 19, 2017A34962
5 results displayed, search above for a specific certificate
Safety Data Sheets
SDSScientific Resources
Product Information
Frequently asked questions (FAQs)
Either method will work in arresting cell division. However, the irradiation process will ensure that cell division will cease regardless of cell aggregation. Cell clumping can potentially not inactivate all cells when using mitomycin C, as cells within clumps may not be exposed to the mitomycin C. Irradiated cells are preferred by those who have concerns about chemical treatment. Mitomycin C-treated cells are preferred by those who have concerns about DNA damage from irradiation.
We recommend seeding these cells at densities ranging from 2 x 10E4 to 5.5 x 10E4 cells/cm2. A good starting point is 3 x 10E4 cells/cm2. If the feeder cells are too sparse, they may not maintain the pluripotent cells without differentiation, and the pluripotent cells may not attach well. If the feeder cells are too dense, the feeder layer may detach from the plate, and the culture will be lost.
These cells should be plated 24 hours prior to plating the ESCs or iPSCs and should be used for only 7-10 days.
Yes, the culture vessel needs to be coated with Attachment Factor protein (Cat. No. S006100) at 37 degrees C for 30 mins or at room temperature for 2 hours. The coated vessels can be used immediately or stored at room temperature for up to 24 hours.