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LV-MAX™ Lentiviral Production System
LV-MAX™ Lentiviral Production System
Gibco™

LV-MAX™ Lentiviral Production System

The Gibco LV-MAX Lentiviral Production System is the first optimized system that provides a scalable and high-yield lentiviral vector productionRead more
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Catalog NumberQuantity
A356841 Kit
Catalog number A35684
Price (USD)
2,405.00
Each
In stock
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Quantity:
1 Kit
Recurring order eligible. Learn more »
Price (USD)
2,405.00
Each
Add to cart
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The Gibco LV-MAX Lentiviral Production System is the first optimized system that provides a scalable and high-yield lentiviral vector production platform. It is based on a suspension, high-density culture of HEK 293-derived Viral Production Cells adapted to a special chemically defined, serum-free and protein-free LV-MAX Production Medium. Optimal viral titer yield is mediated by our advanced lipid nanoparticle transfection reagent in combination with a novel lentiviral-specific enhancer and supplement. All components work synergistically to generate superior and functional lentiviral particles under serum-free conditions compared to conventional polyethylenimine (PEI).

The LV-MAX Lentiviral Production System provides:
• High titer—greater than 1 x 108 TU/mL (unconcentrated LVVs-GFP)
• Scalable suspension system—from 96 deep-well plate to 2-L bioreactor
• Serum-free—eliminating animal-origin components and associated risks
• Easy, robust culture and transfection protocols—fits into current transient production workflows

The components included in the LV-MAX Lentiviral Production System are: two vials of frozen Viral Production Cells, one liter of LV-MAX Production Medium, and one LV-MAX Transfection Kit sufficient to produce 0.3 L of lentivirus. All components of the LV-MAX Lentiviral Production System are also available for purchase separately. Also required, but not included in this kit, is Gibco Opti-MEM I Reduced Serum Medium (Cat. No. 31985062).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineViral Production Cell-Line
Delivery TypeLentiviral, Transfection
Expression SystemTransient
Product LineLV-MAX™
Product TypeLentiviral Production System
Quantity1 Kit
Shipping ConditionApproved for shipment at Room Temperature or on Wet or Dry Ice
Culture TypeSuspension Cell Culture
FormLiquid
Unit SizeEach
Contents & Storage
• 1 L LV-MAX Production Medium. Store at 2–8°C, protect from light.
• 0.3 L LV-MAX Transfection Kit. Store at 2–8°C, protect from light.
• 2 vials of Viral Production Cells. Store in liquid nitrogen until ready to use. Do not store the cells at -80°C.

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Frequently asked questions (FAQs)

What are the major differences between LV-MAX and CTS LV- MAX products?

The LV-MAX and CTS LV- MAX products have equivalent performance. In contrast to the research use only LV-MAX products, CTS LV-MAX products have an additional intended use for cell and gene therapy applications, and are complemented with a more robust regulatory support package to enable clinical therapeutic applications. This means that to the best of our ability, we keep our CTS product specifications and release requirements up-to-date in accordance with the latest regulatory guidance specific for this intended use.

What is the intended use claim for your LV-MAX and CTS LV-MAX products?

Our LV-MAX products are intended for research use only while our CTS LV- MAX products are intended for research use or manufacture of cell, gene or tissue based products. Please refer to the individual products for further details on the intended use claim.

Do you offer a lentivirus production service?

Yes. Please provide your details here (https://www.thermofisher.com/us/en/home/global/forms/life-science/lentiviral-vector-production-get-info.html) and mention your interest in our lentivirus production services. A member of our services team will contact you shortly.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

With the LV-MAX Production System, are the lentiviral particles stable in packaging media?

Lentiviral particles packaged in our LV-MAX Production Medium will be stable at 4 degrees C for 24 hours and will then begin to lose activity. For long-term storage, we recommend storing at –80 degrees C.

With the LV-MAX Production System, can I use cell lines besides HT1080 for titering my lentivirus?

We strongly recommend the human fibrosarcoma line HT1080 (ATCC, Cat. No. CCL-121) as the “gold standard” for reproducibly titering lentivirus. However, you may wish to use the same mammalian cell line to titer your lentiviral stocks as you will use to perform your expression studies (e.g., if you are performing expression studies in a dividing cell line or a non-primary cell line). If you have more than one lentiviral construct, we recommend that you titer all of the lentiviral constructs using the same mammalian cell line.

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Limited Use Label Licenses (LULL)

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Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product and progeny, to perform internal research and development for the sole benefit of the purchaser. The purchase of this product does not grant the purchaser any additional rights, including, without limitation: (a) the right to use the product or its components in commercial applications of any kind, including bioproduction (e.g. use of the product to manufacture therapeutic agents or diagnostic test components, such as proteins, antibodies, viral particles or virus-like particles), quality control and commercial services such as reporting the results of purchaser's activities for a fee or other form of consideration; (b) the right to use the product or its components as a therapeutic agent or diagnostic test component; (c) the right to use the product or its components to produce material for use in human clinical trials; or (d) the right to transfer or resell the product or its components in any form, progeny or derivative. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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