Search Thermo Fisher Scientific
- Contact Us
- Quick Order
-
Don't have an account ? Create Account
Certificates
SDS
Thermo Scientific™
Canine Genotypes Panel 1.1 Kit
Thermo Scientific Canine Genotypes Panel 1.1 is a reagents kit for canine parentage testings and identification using Short Tandem RepeatRead more
| Catalog Number | Quantity |
|---|---|
| F860S |
Catalog number F860S
Price (MXN)Contact Us
-
Thermo Scientific Canine Genotypes Panel 1.1 is a reagents kit for canine parentage testings and identification using Short Tandem Repeat (STR) DNA loci. Due to their high level of informativeness and Mendelian inheritance, microsatellites have become the markers of choice for parentage testing, identification and forensics.
The Canine Genotypes Panel 1.1 provides all the necessary reagents for amplification of the 19 microsatellite loci. The kit contains master mix and primer mix allows co-amplification of the microsatellites in a single multiplex PCR reaction. Canine control DNA is provided to verify acceptable PCR and electrophoresis conditions. Reagents and protocols of the Canine Genotypes Panel 1.1 kit have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the 'plus-A peaks'. One primer from each primer pair is end-labelled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated capillary electrophoresis instrument.
Canine Genotypes Panel 1.1 encompasses the following 19 loci: AHTk211, CXX279, REN169O18, INU055, REN54P11, INRA21, AHT137, REN169D01, AHTh260, AHTk253, INU005, INU030, FH2848, AHT121, FH2054, REN162C04, AHTh171, REN247M23 and Amelogenin (sex determining locus). These markers are included in the 'core panel' of loci recommended by the Applied Genetics Committee of Companying Animals of the International Society for Animal Genetics (ISAG).
Highlights
• Single multiplex PCR of 19 STR loci
• Master mix and Primer mix provided for easy pipetting
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calls represents true alleles (no plus-A peaks)
The Canine Genotypes Panel 1.1 provides all the necessary reagents for amplification of the 19 microsatellite loci. The kit contains master mix and primer mix allows co-amplification of the microsatellites in a single multiplex PCR reaction. Canine control DNA is provided to verify acceptable PCR and electrophoresis conditions. Reagents and protocols of the Canine Genotypes Panel 1.1 kit have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the 'plus-A peaks'. One primer from each primer pair is end-labelled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated capillary electrophoresis instrument.
Canine Genotypes Panel 1.1 encompasses the following 19 loci: AHTk211, CXX279, REN169O18, INU055, REN54P11, INRA21, AHT137, REN169D01, AHTh260, AHTk253, INU005, INU030, FH2848, AHT121, FH2054, REN162C04, AHTh171, REN247M23 and Amelogenin (sex determining locus). These markers are included in the 'core panel' of loci recommended by the Applied Genetics Committee of Companying Animals of the International Society for Animal Genetics (ISAG).
Highlights
• Single multiplex PCR of 19 STR loci
• Master mix and Primer mix provided for easy pipetting
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calls represents true alleles (no plus-A peaks)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodCapillary Electrophoresis
For Use With (Application)PCR
No. of Reactions100
Product TypeCanine Genotypes Panel 1.1 Kit
TargetCanine Genotyping and Parentage Testing
TechniqueSTR (Short Tandem Repeat) Analysis
Unit SizeEach
Contents & Storage
Contains:
• 1.1 mL Canine Genotypes Panel 1.1 Master Mix
• 1.1 mL Canine Genotypes Panel 1.1 Primer Mix
• 30 μL Canine Genotypes Control DNA001
Store at -20°C. Primer Mix should be protected from light at all the times.
• 1.1 mL Canine Genotypes Panel 1.1 Master Mix
• 1.1 mL Canine Genotypes Panel 1.1 Primer Mix
• 30 μL Canine Genotypes Control DNA001
Store at -20°C. Primer Mix should be protected from light at all the times.
Have questions about this product? Ask our AI assisted search.
Generating response
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our
Privacy Notice.
Customers who viewed this item also viewed
Documents & Downloads
Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3267725Certificate of AnalysisJun 25, 2025F860S
3267719Certificate of AnalysisJun 25, 2025F860S
3266931Certificate of AnalysisJun 23, 2025F860S
3264838Certificate of AnalysisJun 19, 2025F860S
3262542Certificate of AnalysisJun 17, 2025F860S
5 results displayed, search above for a specific certificate
Safety Data Sheets
SDSProduct Information
Frequently asked questions (FAQs)
No, extra markers cannot be added to any of the Animal Genotyping kits. All primer mixes are optimized to work in a multiplex PCR. Addition of one or more primers would require optimization of the primer concentrations and the PCR protocol.
We do not provide primers for specific locii separately. They come only in a multiplex format. If one or several loci did not amplify from the sample, faint signals can be a result of low DNA amounts or impurities. Some loci may be more sensitive towards purity of DNA used and may vary from sample to sample. On rare occasions, an animal can have a deletion in the marker region.
No, none of the genotyping kits contain an allelic ladder. Animal STR loci are highly polymorphic. Each allele has too many variants to make a universal allelic ladder.