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Easy-DNA™ gDNA Purification Kit
Easy-DNA™ gDNA Purification Kit
Invitrogen™

Easy-DNA™ gDNA Purification Kit

The Easy-DNA™ Kit is a fast and easy way to isolate high-quality, high-molecular weight genomic DNA (gDNA) from a wideRead more
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Catalog NumberQuantity
K1800011 Kit
Catalog number K180001
Price (USD)
849.65
Online Exclusive
892.00
Save 42.35 (5%)
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Quantity:
1 Kit
Recurring order eligible. Learn more »
Price (USD)
849.65
Online Exclusive
892.00
Save 42.35 (5%)
Each
Add to cart
Ask our AI about this Product
The Easy-DNA™ Kit is a fast and easy way to isolate high-quality, high-molecular weight genomic DNA (gDNA) from a wide range of cells and tissue types. Sample sizes can range from a single hair follicle up to 1 gram of mammalian tissue. Other samples tested include mouse tails, plant leaves, yeast, and E. coli cells. These samples yield high-quality DNA with an average size between 100 kb and 200 kb, which is suitable for PCR, DNA hybridization, genomic DNA library construction, and other applications. Advantages of the Easy-DNA™ Kit include:

Reliability—high-quality genomic DNA from large or small samples of cells, tissue, plant, yeast, E. coli, blood, and even hair follicles
Simplicity—for most sample types, only 4 steps, typically requiring less than 90 minutes are needed to isolate high-quality DNA
Versatility—one kit is supported by optimized protocols for a variety of cell and tissue types

Simple workflow, quality results
Optimized protocols for a variety of cell and tissue types are included in the instruction manual supplied with the kit. Generally, small-scale gDNA preparations are performed in microfuge tubes, in which samples are mixed with Lysis Solution and incubated at 65°C for 10 minutes. After extraction with chloroform (not included in kit), precipitation, and reconstitution of the DNA pellet, the preparation is complete. The kit contains a protein degradation reagent (for use with high-protein–containing samples such as connective tissue), mussel glycogen for use as a co-precipitant for dilute DNA samples, and an RNase solution.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume10 to 150 μL
Final Product TypegDNA
For Use With (Application)PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Quantity1 Kit
Sample TypeBacteria, Cells, Hair Follicles, Mouse Tails, Plant, Tissue, Virus, Whole Blood, Yeast
Shipping ConditionRoom Temperature
Starting Material AmountBacteria: ≤109 cells
Cells: ≤108
Hair Follicles: ≤5
Mouse Tails: 1 cm
Plant: ≤50 mg
Tissue: ≤1 g
Virus: ≤750 μL
Whole Blood: ≤2 mL
Yeast: ≤10 mL
Test TimeUp to 90 min.
Yield≤850 μg
Isolation TechnologyOrganic Extraction
Unit SizeEach
Contents & Storage
• 55 mL Solution A; room temperature
• 25 mL Solution B; room temperature
• 100 mL TE Buffer; room temperature
• 750 μL Mussel Glycogen; room temperature, or -20°C for long-term storage
• 750 μL RNase; room temperature, or -20°C for long-term storage
• 750 μL Protein Degrader; room temperature, or -20°C for long-term storage

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Frequently asked questions (FAQs)

What type of samples can be used for the isolation of DNA using the Easy-DNA Kit?

DNA from the following sources has been isolated and successfully used to produce PCR products or in Southern blot experiments:

- Fresh, dried, frozen, or heparinized blood
- Tissue culture cells, both suspended and trypsinized
- Mammalian tissue
- E.coli
- Yeast cells
- Plant leaves
- Hair follicles
- Mouse tails
- Baculovirus (viral particles)

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Citations & References (20)

Citations & References
Abstract
Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection.
Authors:Zhang Z, Theurkauf WE, Weng Z, Zamore PD,
Journal:Silence
PubMed ID:23273270
'High throughput DNA sequencing technology has enabled quantification of all the RNAs in a cell or tissue, a method widely known as RNA sequencing (RNA-Seq). However, non-coding RNAs such as rRNA are highly abundant and can consume >70% of sequencing reads. A common approach is to extract only polyadenylated mRNA; ... More
Participation of fad and mbt genes in synthesis of mycobactin in Mycobacterium smegmatis.
Authors:LaMarca BB, Zhu W, Arceneaux JE, Byers BR, Lundrigan MD,
Journal:J Bacteriol
PubMed ID:14702306
'Colonies of Mycobacterium smegmatis LR222 on iron-limiting (0.1 micro M Fe) minimal medium agar fluoresce under UV light due to the accumulation in the cells of the deferri form of the siderophore mycobactin. Two mutants with little or no fluorescence, designated LUN8 and LUN9, were isolated by screening colonies of ... More
Resistance to murine AIDS in offspring of mice infected with LP-BM5. Role of CD8 T cells.
Authors:Pavlovitch JH, Hulier E, Rizk-Rabin M, Marussig M, Mazier D, Joffret ML, Hoos S, Papiernik M
Journal:J Immunol
PubMed ID:8648122
'The murine-acquired immunodeficiency syndrome (MAIDS) is caused by a mixture of murine leukemia viruses (LP-BM5 MuLV). The influence of perinatal contact with retroviruses or their Ags on the response to infection was tested by infecting with LP-BM5 (MuLV) the adult offspring of mice with MAIDS. These offspring were resistant to ... More
Inducible expression of a dominant negative DNA polymerase-gamma depletes mitochondrial DNA and produces a rho0 phenotype.
Authors:Jazayeri M, Andreyev A, Will Y, Ward M, Anderson CM, Clevenger W,
Journal:J Biol Chem
PubMed ID:12645575
'We report the inducible, stable expression of a dominant negative form of mitochondria-specific DNA polymerase-gamma to eliminate mitochondrial DNA (mtDNA) from human cells in culture. HEK293 cells were transfected with a plasmid encoding inactive DNA polymerase-gamma harboring a D1135A substitution (POLGdn). The cells rapidly lost mtDNA (t1/2 = 2-3 days) ... More
Characterization and sequencing of prototypic human T-lymphotropic virus type 1 (HTLV-1) from and HTLV-1/2 seroindeterminate patient.
Authors:Waziri A, Soldan SS, Graf MD, Nagle J, Jacobson S
Journal:J Virol
PubMed ID:10666247
'Serological screening for human T-lymphotropic virus type 1 (HTLV-1) parallels the standard screening process for human immunodeficiency virus (HIV), in which samples found positive by enzyme-linked immunosorbent assay (ELISA) are confirmed with a modified Western blot procedure. There are a significant number of cases in which HTLV-1/2 ELISA-positive specimens demonstrate ... More
20 total citations

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