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SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells
SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells
Invitrogen™

SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells

SYTOX Deep Red Nucleic Acid Stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranesRead more
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Catalog NumberQuantity
S11381Promo Image5 x 50 μL
S11380Promo Image50 μL
Catalog number S11381
Price (USD)
690.65
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720.00
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5 x 50 μL
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Price (USD)
690.65
Online Exclusive
720.00
Save 29.35 (4%)
Each
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SYTOX Deep Red Nucleic Acid Stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes and yet does not cross the membranes of live cells. With minimal cytoplasmic staining, SYTOX Deep Red stain is particularly useful in immunocytochemistry (ICC), immunohistochemistry (IHC), and immunofluorescence (IF) experiments for staining of nuclei of fixed cells. After a brief incubation with SYTOX Deep Red stain, the nucleic acids of dead or fixed cells fluoresce a bright, deep red/far red and can be detected with a CY5/Deep Red standard filter set or laser configuration.

Features of SYTOX Deep Red Nucleic Acid Stain include:
• Impermeant to live cells; permeant to dead or fixed cells
• Excitation/emission peak: 660/682 nm
• Use with Cy5 traditional filter set or 647 laser line
• Works with mammalian cells in mono layer or 3D cell culture, animal tissue, and bacteria
• Detectable with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers

SYTOX Deep Red Nucleic Acid Stain is multiplexable with blue, green, orange, red, and near IR fluorophores, when compatible fluorescent filter/laser configurations are used. SYTOX Deep Red stain shows increased fluorescence with increasing concentrations of dsDNA, but does not show much affinity for RNA or ssDNA. It has a bright initial signal and excellent photo stability in a typical imaging experiment. These properties make SYTOX Deep Red stain a simple and quantitative single-step dead/fixed cell nucleus-labeling dye for use with fluorescence micro¬scopes, fluorimeters, fluorescence microplate readers, and flow cytometers.

SYTOX Deep Red Nucleic Acid Stain had been used successfully in mono-layer cells, thin-sliced tissue, thick-sliced tissue, 3D cell cultures/spheroids, and bacteria. It is supplied at a 1 mM concentration in DMSO (∼2000X concentration). A concentration of 0.5 μM (1X) is recommended for most applications and cell types. In fluorescent imaging of various mammalian cells, a concentration range of 2.5 μM to 0.25 μM has been used. For viability testing by flow cytometry, a concentration of 1 μM to 10 nM has been used for Jurkat cells and a range of 4 μM to 0.5 μM has been used with E. coli.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorDeep Red
Detection MethodFluorescence
Dye TypeCell-Permeant
Emission682 nm
Excitation Wavelength Range660 nm
For Use With (Application)Viability Assay
For Use With (Equipment)Fluorescence Microscope
Product LineSYTOX
Quantity5 x 50 μL
Shipping ConditionApproved for shipment on Wet or Dry Ice
Product TypeNucleic Acid Stain
SubCellular LocalizationNucleic Acids
Unit SizeEach
Contents & Storage
Store in freezer at -5°C to -30°C and protect from light.
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Frequently asked questions (FAQs)

Can I use SYTOX Deep Red Nucleic Acid Stain with a cell permeable nucleic acid stain for live/dead cell analysis?

Yes. For live cell staining, you may use any of the blue, green, or orange SYTO dyes or any of the blue fluorescent Hoechst stains.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use SYTOX Deep Red Nucleic Acid Stain for cell cycle analysis?

We do not recommend using SYTOX Deep Red Nucleic Acid Stain for cell cycle analysis. Instead, we recommend using Vybrant DyeCycle Ruby Stain (Cat Nos. V10273, V10309).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is SYTOX Deep Red Nucleic Acid Stain, for fixed/dead cells (Cat. Nos. S11380, S11381) different from SYTOX Red Dead Cell Stain (Cat No. S34859)?

Although they are both far red, cell impermeant nucleic acid stains, there is approximately a 20 nm difference in the excitation and emission maxima: SYTOX Deep Red Nucleic Acid Stain ex/em = 660/682 nm and SYTOX Red Dead Cell Stain ex/em = 640/658. In addition, the main difference is that SYTOX Deep Red Nucleic Acid Stain has less propensity to stain RNA compared to SYTOX Red Dead Cell Stain.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3123559Certificate of AnalysisMar 17, 2025S11381
2994108Certificate of AnalysisSep 28, 2024S11380
2916338Certificate of AnalysisMay 30, 2024S11381
2860809Certificate of AnalysisFeb 01, 2024S11380
2685315Certificate of AnalysisJul 24, 2023S11381
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Citations & References (2)

Citations & References
Abstract
Antibody/Ligand-Target Receptor Internalization Assay Protocol Using Fresh Human or Murine Tumor
Authors:Joseph SR, Lum B, Banushi B, Barry R, Panizza B, Walpole E, Simpson F
Journal:STAR Protoc
PubMed ID:33111120
We describe an
Multiplexed imaging of nucleome architectures in single cells of mammalian tissue.
Authors:Liu M, Lu Y, Yang B, Chen Y, Radda JSD, Hu M, Katz SG, Wang S
Journal:Nat Commun
PubMed ID:32518300
The three-dimensional architecture of the genome affects genomic functions. Multiple genome architectures at different length scales, including chromatin loops, domains, compartments, and lamina- and nucleolus-associated regions, have been discovered. However, how these structures are arranged in the same cell and how they are mutually correlated in different cell types in ... More
2 total citations

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