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Invitrogen™
SYBR™ Green I Nucleic Acid Gel Stain, 10,000X concentrate in DMSO
SYBR™ Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA)Read more
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| Catalog Number | Quantity |
|---|---|
| S7563 | 500 μL |
| S7585 | 20 x 50 μL |
| S7567 | 1 mL |
Catalog number S7563
Price (USD)
463.65
Online Exclusive
497.00Save 33.35 (7%)
Each
In stock
Quantity:
500 μL
Price (USD)
463.65
Online Exclusive
497.00Save 33.35 (7%)
Each
SYBR™ Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels. Because SYBR™ Green I has greater sensitivity for dsDNA, it is especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. With exceptionally low background fluorescence and spectral characteristics that closely match light sources and filter sets in existing instruments, SYBR™ Green I stain is ideal for use with laser scanners. SYBR™ Green I stain provides:
• Sensitivity—at least four times greater than ethidium bromide for DNA in agarose gels
• Ease of use—gels soaked in diluted stain can be visualized without desalting
• Compatibility—with UV transilluminators, gel documentation systems, and laser scanners
• Flexibility—for use with a broad range of applications, including DNA typing, PCR-based assays, DNA damage assays, analysis of complex samples, and real-time PCR detection
Product use
One mL stains 100 minigels. Various pack sizes are available.
• Sensitivity—at least four times greater than ethidium bromide for DNA in agarose gels
• Ease of use—gels soaked in diluted stain can be visualized without desalting
• Compatibility—with UV transilluminators, gel documentation systems, and laser scanners
• Flexibility—for use with a broad range of applications, including DNA typing, PCR-based assays, DNA damage assays, analysis of complex samples, and real-time PCR detection
Product use
One mL stains 100 minigels. Various pack sizes are available.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodFluorescence
Product TypeNucleic Acid Gel Stain
Quantity500 μL
Shipping ConditionRoom Temperature
Target MoleculeDNA
Label or DyeSYBR Green I
Unit SizeEach
Contents & Storage
• Supplied as a 10,000X concentrate in DMSO
Store at -20°C, protected from light in a dessicator.
Store at -20°C, protected from light in a dessicator.
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Figures
DNA molecular weight ladders stained with SYBR® Green I Nucleic Acid Gel Stain.
Electrophoretic bandshift assay using SYBR® Green I Nucleic Acid Gel Stain.
Comparison of single-stranded oligonucleotide detection using SYBR® Green I Nucleic Acid Gel Stain and ethidium bromide.
HL-60 cells in a comet assay.
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Lot #Certificate TypeDateCatalog Number(s)
3143092Certificate of AnalysisApr 04, 2025S7563
2994111Certificate of AnalysisSep 09, 2024S7585
2980704Certificate of AnalysisAug 12, 2024S7567
2806848Certificate of AnalysisDec 31, 2023S7585
2720336Certificate of AnalysisNov 06, 2023S7563
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Citations & References (466)
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Citations & References
Abstract
A rapid and sensitive approach to mutation detection using real-time polymerase chain reaction and melting curve analyses, using BRCA1 as an example.
Journal:Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology
PubMed ID:10553024
Comparison of multiple DNA dyes for real-time PCR: effects of dye concentration and sequence composition on DNA amplification and melting temperature.
Journal:Nucleic acids research
PubMed ID:17897966
The importance of real-time polymerase chain reaction (PCR) has increased steadily in clinical applications over the last decade. Many applications utilize SYBR Green I dye to follow the accumulation of amplicons in real time. SYBR Green I has, however, a number of limitations that include the inhibition of PCR, preferential
Diagnosis of herpes simplex virus infections in the clinical laboratory by LightCycler PCR.
Journal:Journal of clinical microbiology
PubMed ID:10655387
Herpes simplex virus (HSV) causes several clinical manifestations in both normal and immunocompromised hosts; this agent is the most frequently detected virus in diagnostic laboratories. Recovery of the virus in cell culture is considered the âgold standardâ for detection of this virus from sources other than cerebrospinal fluid. LightCycler is
Kruppel-like factor 2 (KLF2) regulates proinflammatory activation of monocytes.
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:16617118
The mechanisms regulating activation of monocytes remain incompletely understood. Herein we provide evidence that Kruppel-like factor 2 (KLF2) inhibits proinflammatory activation of monocytes. In vitro, KLF2 expression in monocytes is reduced by cytokine activation or differentiation. Consistent with this observation, KLF2 expression in circulating monocytes is reduced in patients with
Characterization of a new NIH-registered variant human embryonic stem cell line, BG01V: a tool for human embryonic stem cell research.
Journal:Stem cells (Dayton, Ohio)
PubMed ID:16293579
466 total citations