Vybrant™ DiI Cell-Labeling Solution
Vybrant™ DiI Cell-Labeling Solution
Invitrogen™

Vybrant™ DiI Cell-Labeling Solution

DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporatedRead more
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Catalog NumberQuantity
V22885Promo Image1 mL
Catalog number V22885
Price (USD)
200.00
1 mL
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1 mL
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Price (USD)
200.00
1 mL
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Ask our AI about this Product
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solid (D-282), as a paste (N-22880) or as crystals (D-3911).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorRed-Orange
Detection MethodFluorescence
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product LineVybrant™
Quantity1 mL
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeCell Labeling
SubCellular LocalizationCell Membranes, Lipids
Unit Size1 mL
Contents & Storage
Store at room temperature and protect from light.
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Frequently asked questions (FAQs)

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

This is expected. DiD (which is far-red fluorescent) is never as uniform as DiI (which is orange fluorescent). If uniformity is desired, try increasing the label time and concentration, but it still isn't likely to be as uniform as DiI. CellMask Deep Red Plasma Membrane stain is much more uniform and is about the same wavelength as DiD. However, if you intend to do cell tracking over days, CellMask stain has not been tried for that application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to perform a cell fusion assay, where one cell line is labeled with one color and the other cell line with another color, and combine with a nucleic acid stain. What do you recommend?

A typical method is to label one cell line with orange fluorescent DiI C18 and the other cell line with green fluorescent DiO C18. These orange and green lipophilic cyanine dyes will stain the membranes of cells. Cells that fuse will then have both dyes, yielding a yellow color (when images are overlaid or cells are imaged in a dual-bandpass filter). These live cells can then be labeled with Hoechst 33342 (a cell-permeant blue DNA stain comparable in wavelength to DAPI), but only as an endpoint just before imaging (since DNA stains can interrupt DNA function).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I need to look at live cell morphology deformation over the course of a few hours. What sort of membrane dye would be useful for this?

Lipophilic cyanine dyes, such as DiI (Cat. No. D282), DiO (Cat. No. D275), DiD (Cat. No. D7757) or DiR (Cat. No. D12731), are commonly used. The longer the alkyl chain on the dye, the better the retention in lipophilic environments.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How do I know which tracer to choose for my experiment?

Factors to consider are size of tracer, method of delivery (injection, direct application to tissue, etc.), and if the tracer needs to be fixable. Here are some links to details about the various classes of neuronal tracers we offer and how to choose between them:

Neuronal Tracing (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html)
Choosing a Tracer (https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/fluorescent-tracers-of-cell-morphology-and-fluid-flow/choosing-a-tracer.html)
Imaging Analysis (http://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/bioprobes-50-journal.pdf)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to track my cells with a nucleic acid stain, like DAPI or Hoechst dye. Do you recommend this?

This is not recommended. When these stains bind to DNA and RNA, they may affect the normal function of the nucleic acids, disrupting transcription, as well as replication. Other reagents, such as CellTracker dyes or Qtracker reagents are more optimized for tracking without disrupting normal activity. If a nuclear label is still desired, though, and the cells are mammalian and non-hematopoietic, CellLight nuclear reagents can transiently transfect cells to express GFP or RFP on a nuclear-expressing protein for up to several days without affecting function.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

Figures

Fluorescence spectra

Fluorescence spectra

Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
3011752Certificate of AnalysisNov 15, 2024V22885
2743097Certificate of AnalysisNov 13, 2023V22885
2513388Certificate of AnalysisSep 06, 2022V22885
2369088Certificate of AnalysisAug 10, 2021V22885
2286884Certificate of AnalysisDec 23, 2020V22885
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Safety Data Sheets

Citations & References (155)

Citations & References
Abstract
Limb bud mesenchyme permits seminiferous cord formation in the mouse fetal testis but subsequent testosterone output is markedly affected by the sex of the donor stromal tissue.
Authors:Moreno-Mendoza N, Herrera-Muñoz J, Merchant-Larios H
Journal:Dev Biol
PubMed ID:7750656
Mesonephric stromal cells have previously been shown to migrate into the genital ridge and to be necessary for seminiferous cord formation in organ culture. Here, we asked whether there is a specific requirement for mesonephric stromal cells or whether another source of mesonephric stromal cells can be substituted. Hindlimb buds ... More
Evaluation of a flow cytometric fluorescence quenching assay of phagocytosis of sensitized sheep erythrocytes by polymorphonuclear leukocytes.
Authors:Van Amersfoort ES, Van Strijp JA
Journal:Cytometry
PubMed ID:7875036
A number of reports have been published describing phagocytosis assays for flow cytometric analysis. In some of these, the fluorescence quenching technique has been used to discriminate between adherent and ingested particles. In this report, we have evaluated the efficacy of a quantitative fluorescence quenching technique with crystal violet and ... More
The use of the lipophilic fluorochrome CM-DiI for tracking the migration of lymphocytes.
Authors:Andrade W, Seabrook TJ, Johnston MG, Hay JB
Journal:J Immunol Methods
PubMed ID:8765171
'In this study we examined the new cell dye CM-DiI for tracking the migration of lymphocytes from blood to lymph. This lipophilic marker intercalates in the plasma membrane like the PKH dyes and older DiI derivatives. The stability and intensity of staining achieved with these dyes is better than most ... More
Mechanisms of adoptive immunotherapy: improved methods for in vivo tracking of tumor-infiltrating lymphocytes and lymphokine-activated killer cells.
Authors:Wallace PK, Palmer LD, Perry-Lalley D, Bolton ES, Alexander RB, Horan PK, Yang JC, Muirhead KA
Journal:Cancer Res
PubMed ID:8485722
'Adoptive immunotherapy with tumor-infiltrating lymphocytes (TIL) and lymphokine-activated killer cells has been demonstrated to mediate regression of tumors in murine models and in selected patients with advanced cancer. Improved methods for monitoring immune cell traffic, particularly to sites of tumor, are needed to elucidate mechanisms of antitumor activity and optimize ... More
A flow cytometric method to assess antigen-specific proliferative responses of different subpopulations of fresh and cryopreserved human peripheral blood mononuclear cells.
Authors:Allsopp CE, Nicholls SJ, Langhorne J
Journal:J Immunol Methods
PubMed ID:9692869
'We have used PKH26 dye, which is incorporated stably into the membrane of cells, to determine, using flow cytometry, lymphocyte proliferative responses to the antigen tetanus toxoid in fresh and cryopreserved samples. Measuring cell proliferation with this dye has advantages over either 3H-thymidine or Bromodeoxyuridine (BrdU). Whereas the existing methods ... More
155 total citations

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