Wheat Germ Agglutinin (WGA)
Wheat Germ Agglutinin (WGA)
Invitrogen™

Wheat Germ Agglutinin (WGA)

Thermo Fisher Scientific offers bright conjugates of wheat germ agglutinin (WGA) and Alexa Fluor, Alexa Fluor Plus, and other dyes. Fluorescent WGA conjugates bind to carbohydrates and are used for various cell biology applications such as plasma membrane labeling and cell painting assays.
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Catalog NumberConjugate
W7024Promo ImageAlexa Fluor 350, Oregon Green 488, Tetramethylrhodamine, Texas Red
W11263Promo ImageAlexa Fluor 350
W11261Promo ImageAlexa Fluor 488
W32464Promo ImageAlexa Fluor 555
W11262Promo ImageAlexa Fluor 594
W21404Promo ImageAlexa Fluor 633
W32466Promo ImageAlexa Fluor 647
W32465Promo ImageAlexa Fluor 680
W56132Promo ImageAlexa Fluor Plus 405
W56133Promo ImageAlexa Fluor Plus 568
W56134Promo ImageAlexa Fluor Plus 770
W834Promo ImageFluorescein
W6748Promo ImageOregon Green 488
Q12021MPPromo ImageQdot 655
W849Promo ImageTetramethylrhodamine
W21405Promo ImageTexas Red-X
Catalog number W7024
Price (USD)
400.65
Online Exclusive
411.00
Save 10.35 (3%)
1 kit
In stock
Add to cart
Conjugate:
Alexa Fluor 350, Oregon Green 488, Tetramethylrhodamine, Texas Red
Recurring order eligible. Learn more »
Price (USD)
400.65
Online Exclusive
411.00
Save 10.35 (3%)
1 kit
Add to cart
Ask our AI about this Product

Thermo Fisher Scientific offers a broad selection of fluorescent wheat germ agglutinin conjugates. These lectins can bind to carbohydrates and are available conjugated to Alexa Fluor™, Alexa Fluor™ Plus, and other fluorescent dyes. Fluorescent wheat germ agglutinin conjugates are valuable tools in molecular and cell biology research, enabling researchers to label the plasma membrane in fluorescence imaging and cell painting assays, and study and analyze glycosylation patterns and glycan-mediated processes in cells and tissues.

Thermo Fisher Scientific offers bright conjugates of wheat germ agglutinin (WGA) with Alexa Fluor, Alexa Fluor Plus, and other dyes. WGA is a cell impermeant stain that selectively binds to N‐acetylglucosamine and N‐acetylneuraminic acid (sialic acid) residues, which are often found on cell membranes. Fluorescent WGA conjugates provide selective labeling of the plasma membrane with minimal background in many cell types that is retained after formaldehyde fixation and permeabilization with Triton X-100.

These fluorescent lectin conjugates can also be used to label fixed cells; however, to avoid labeling intracellular components, formaldehyde-fixed cells should not be permeabilized before labeling. Fluorescent WGA conjugates are used as plasma membrane stains along with other cellular markers in cell painting assays to provide a phenotypic readout of cell health or cytotoxicity. The Wheat Germ Agglutinin, Alexa Fluor 555 Conjugate is included in the Image-iT Cell Painting Kit (Cat. Nos. I65000 and I65500).

WGA conjugates are also used as retrograde tracers for neuronal tracing experiments and have been shown to cross synapses. These fluorescent lectins are applicable in microbiology studies to label yeast bud scars, the cell membrane of gram-positive but not gram-negative bacteria, and chitin in fungal cell walls. In solution, WGA exists as a heterodimer with a molecular weight of approximately 38,000 Daltons and is normally cationic under physiological conditions. Our WGA conjugates have been used in variety of applications, including immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), and a wide range of chemical, biochemical and immunological assays.

Thermo Fisher Scientific offers a broad selection of fluorescent wheat germ agglutinin conjugates with options covering the entire wavelength range. The Wheat Germ Agglutinin Sampler Kit (Cat. No. W7024) includes introductory samples of four fluorescent WGAs: Alexa Fluor 350, Oregon Green 488, tetramethylrhodamine, and Texas Red-X conjugates. The red-fluorescent Alexa Fluor 594 wheat germ agglutinin (WGA) conjugate is also included in the Image-iT LIVE Plasma Membrane and Nuclear Labeling Kit (Cat. No. I34406). The Texas Red-X WGA conjugate can be purchased in the ViaGram Red+ Bacterial Gram Stain and Viability Kit (Cat. No. V7023) to differentiate gram-positive and gram-negative bacteria.

Wheat germ agglutinin (WGA) conjugates fluorescence excitation/emission
W11261 WGA, Alexa Fluor 488 conjugate: 495 nm/519 nm
W11263 WGA, Alexa Fluor 350 conjugate: 346 nm/442 nm
W32464 WGA, Alexa Fluor 555 conjugate: 555 nm/580 nm
W11262 WGA, Alexa Fluor 594 conjugate: 590 nm/617 nm
W21404 WGA, Alexa Fluor 633 conjugate: 632 nm/647 nm
W32466 WGA, Alexa Fluor 647 conjugate: 650 nm/665 nm
W32465 WGA, Alexa Fluor 680 conjugate: 679 nm/702 nm
W56132 WGA, Alexa Fluor Plus 405 conjugate: 408 nm/450 nm
W56133 WGA, Alexa Fluor Plus 568 conjugate: 562 nm/583 nm
W56134 WGA, Alexa Fluor Plus 770 conjugate: 770 nm/797 nm
W834 WGA, fluorescein conjugate: 494 nm/518 nm
W6748 WGA, Oregon Green 488 conjugate: 496 nm/524 nm
W849 WGA, tetramethylrhodamine conjugate: 555 nm/580 nm
W21405 WGA, Texas Red-X conjugate: 595 nm/615 nm

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Expression SystemWheat germ
Ligand TypeN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
ProteinFluorescent lectins
Protein FamilyLectins
Protein FormHeterodimer
Protein SubtypeAgglutinin
Protein TagNone
Purity or Quality GradeSee Certificate of Analysis
Quantity1 mg kit
Shipping ConditionRoom Temperature
SourceWheat germ
ConjugateAlexa Fluor 350, Oregon Green 488, Tetramethylrhodamine, Texas Red
For Use With (Application)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
FormLyophilized
RecombinantRecombinant
SpeciesWheat
Unit Size1 kit
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
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Frequently asked questions (FAQs)

My cells are very sensitive and need to be kept in media as much as possible. Is it possible to label the plasma membrane with fluorescent wheat germ agglutinin (WGA) in media instead of buffer?

Yes. Although labeling in buffer (such as Hank's Buffered Saline Solution) is slightly better for brightness and lower non-cell background, media can be used. Do a concentration range to dertermine optimal conditions, since the WGA may potentially bind media components to some extent, slightly decreasing your specific labeling intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I want to label the plasma membrane of my cells, but there are several dyes to choose from. Which one should I use?

For live-cell imaging, the CellVue and CellMask Plasma Membrane Stains are the most uniform and the slowest to be endocytosed. However, they are not the best choice if you wish to fix and permeabilize your cells, such as for antibody labeling. Wheat germ agglutinin (WGA) conjugates are also able to label live cells, or can label already formaldehyde-fixed cells. They can survive subsequent permeabilization with detergents, such as Triton X-100. If cells are already permeabilized, WGA will label internal structures as well. Thus, only an antibody against a plasma membrane protein can be used if cells are already permeabilized. Lipophilic cyanine dyes, such as DiI, will label all cell membranes in live cells, not just plasma membranes, if left on live cells for extended periods. Following page will help you choose (http://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-structure/plasma-membrane.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3212492Certificate of AnalysisJun 17, 2025W56132
3173269Certificate of AnalysisMay 12, 2025W849
3148257Certificate of AnalysisApr 14, 2025Q12021MP
3029766Certificate of AnalysisApr 09, 2025W11261
3110690Certificate of AnalysisMar 30, 2025W32464
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Safety Data Sheets

Citations & References (389)

Citations & References
Abstract
Flow cytometric analysis of human erythrocytes: II. Possible identification of senescent RBC with fluorescently labelled wheat germ agglutinin.
Authors:Rolfes-Curl A, Ogden LL, Omann GM, Aminoff D
Journal:Exp Gerontol
PubMed ID:1936192
In the first paper of a series (Gutowski, et al., 1991) we discussed the use of flow cytometry to follow at the cellular level the aging of red blood cells (RBC) in circulation, using fluorescently labelled lectins and goat anti-human-IgG and -IgM. The Coulter Epics 541 was used for those ... More
Direct priming of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes.
Authors:Hickman HD, Takeda K, Skon CN, Murray FR, Hensley SE, Loomis J, Barber GN, Bennink JR, Yewdell JW,
Journal:Nat Immunol
PubMed ID:18193049
'It is uncertain how antiviral lymphocytes are activated in draining lymph nodes, the site where adaptive immune responses are initiated. Here, using intravital microscopy we show that after infection of mice with vaccinia virus (a large DNA virus) or vesicular stomatitis virus (a small RNA virus), virions drained to the ... More
Essential role of protein kinase C delta in platelet signaling, alpha IIb beta 3 activation, and thromboxane A2 release.
Authors:Yacoub D, Théorêt JF, Villeneuve L, Abou-Saleh H, Mourad W, Allen BG, Merhi Y
Journal:J Biol Chem
PubMed ID:16895913
'The protein kinase C (PKC) family is an essential signaling mediator in platelet activation and aggregation. However, the relative importance of the major platelet PKC isoforms and their downstream effectors in platelet signaling and function remain unclear. Using isolated human platelets, we report that PKCdelta, but not PKCalpha or PKCbeta, ... More
Lipid-dependent bidirectional traffic of apolipoprotein B in polarized enterocytes.
Authors:Morel E, Demignot S, Chateau D, Chambaz J, Rousset M, Delers F
Journal:Mol Biol Cell
PubMed ID:14565984
'Enterocytes are highly polarized cells that transfer nutrients across the intestinal epithelium from the apical to the basolateral pole. Apolipoprotein B (apoB) is a secretory protein that plays a key role in the transepithelial transport of dietary fatty acids as triacylglycerol. The evaluation of the control of apoB traffic by ... More
Synaptic vesicle proteins and early endosomes in cultured hippocampal neurons: differential effects of Brefeldin A in axon and dendrites.
Authors:Mundigl O, Matteoli M, Daniell L, Thomas-Reetz A, Metcalf A, Jahn R, De Camilli P
Journal:J Cell Biol
PubMed ID:8376458
'The pathways of synaptic vesicle (SV) biogenesis and recycling are still poorly understood. We have studied the effects of Brefeldin A (BFA) on the distribution of several SV membrane proteins (synaptophysin, synaptotagmin, synaptobrevin, p29, SV2 and rab3A) and on endosomal markers to investigate the relationship between SVs and the membranes ... More
389 total citations

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