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Wheat Germ Agglutinin (WGA)
Wheat Germ Agglutinin (WGA)
Invitrogen™

Wheat Germ Agglutinin (WGA)

Thermo Fisher Scientific offers bright conjugates of wheat germ agglutinin (WGA) and Alexa Fluor, Alexa Fluor Plus, and other dyes. Fluorescent WGA conjugates bind to carbohydrates and are used for various cell biology applications such as plasma membrane labeling and cell painting assays.
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Número de catálogoConjugado
W11262Alexa Fluor 594
W11261Alexa Fluor 488
W32464Alexa Fluor 555
W56132Alexa Fluor Plus 405
W56133Alexa Fluor Plus 568
W56134Alexa Fluor Plus 770
Número de catálogo W11262
Precio (USD)
-
Conjugado:
Alexa Fluor 594
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Thermo Fisher Scientific offers a broad selection of fluorescent wheat germ agglutinin conjugates. These lectins can bind to carbohydrates and are available conjugated to Alexa Fluor™, Alexa Fluor™ Plus, and other fluorescent dyes. Fluorescent wheat germ agglutinin conjugates are valuable tools in molecular and cell biology research, enabling researchers to label the plasma membrane in fluorescence imaging and cell painting assays, and study and analyze glycosylation patterns and glycan-mediated processes in cells and tissues.

Thermo Fisher Scientific offers bright conjugates of wheat germ agglutinin (WGA) with Alexa Fluor, Alexa Fluor Plus, and other dyes. WGA is a cell impermeant stain that selectively binds to N‐acetylglucosamine and N‐acetylneuraminic acid (sialic acid) residues, which are often found on cell membranes. Fluorescent WGA conjugates provide selective labeling of the plasma membrane with minimal background in many cell types that is retained after formaldehyde fixation and permeabilization with Triton X-100.

These fluorescent lectin conjugates can also be used to label fixed cells; however, to avoid labeling intracellular components, formaldehyde-fixed cells should not be permeabilized before labeling. Fluorescent WGA conjugates are used as plasma membrane stains along with other cellular markers in cell painting assays to provide a phenotypic readout of cell health or cytotoxicity. The Wheat Germ Agglutinin, Alexa Fluor 555 Conjugate is included in the Image-iT Cell Painting Kit (Cat. Nos. I65000 and I65500).

WGA conjugates are also used as retrograde tracers for neuronal tracing experiments and have been shown to cross synapses. These fluorescent lectins are applicable in microbiology studies to label yeast bud scars, the cell membrane of gram-positive but not gram-negative bacteria, and chitin in fungal cell walls. In solution, WGA exists as a heterodimer with a molecular weight of approximately 38,000 Daltons and is normally cationic under physiological conditions. Our WGA conjugates have been used in variety of applications, including immunofluorescence (IF), immunohistochemistry (IHC), flow cytometry (FC), and a wide range of chemical, biochemical and immunological assays.

Thermo Fisher Scientific offers a broad selection of fluorescent wheat germ agglutinin conjugates with options covering the entire wavelength range. The Wheat Germ Agglutinin Sampler Kit (Cat. No. W7024) includes introductory samples of four fluorescent WGAs: Alexa Fluor 350, Oregon Green 488, tetramethylrhodamine, and Texas Red-X conjugates. The red-fluorescent Alexa Fluor 594 wheat germ agglutinin (WGA) conjugate is also included in the Image-iT LIVE Plasma Membrane and Nuclear Labeling Kit (Cat. No. I34406). The Texas Red-X WGA conjugate can be purchased in the ViaGram Red+ Bacterial Gram Stain and Viability Kit (Cat. No. V7023) to differentiate gram-positive and gram-negative bacteria.

Wheat germ agglutinin (WGA) conjugates fluorescence excitation/emission
W11261 WGA, Alexa Fluor 488 conjugate: 495 nm/519 nm
W11263 WGA, Alexa Fluor 350 conjugate: 346 nm/442 nm
W32464 WGA, Alexa Fluor 555 conjugate: 555 nm/580 nm
W11262 WGA, Alexa Fluor 594 conjugate: 590 nm/617 nm
W21404 WGA, Alexa Fluor 633 conjugate: 632 nm/647 nm
W32466 WGA, Alexa Fluor 647 conjugate: 650 nm/665 nm
W32465 WGA, Alexa Fluor 680 conjugate: 679 nm/702 nm
W56132 WGA, Alexa Fluor Plus 405 conjugate: 408 nm/450 nm
W56133 WGA, Alexa Fluor Plus 568 conjugate: 562 nm/583 nm
W56134 WGA, Alexa Fluor Plus 770 conjugate: 770 nm/797 nm
W834 WGA, fluorescein conjugate: 494 nm/518 nm
W6748 WGA, Oregon Green 488 conjugate: 496 nm/524 nm
W849 WGA, tetramethylrhodamine conjugate: 555 nm/580 nm
W21405 WGA, Texas Red-X conjugate: 595 nm/615 nm

For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Nombre comúnWheat Germ Agglutinin, Alexa Fluor™ 594 Conjugate
Sistema de expresiónWheat germ
Tipo de ligandoN-acetylglucosamine and N-acetylneuraminic acid (sialic acid) residues
Línea de productosAlexa Fluor™
ExpresiónFluorescent lectins
Familia de proteínasLectins
Forma de proteínaHeterodimer
Subtipo de proteínaAgglutinin
Etiqueta de proteínaNone
Grado de pureza o calidadSee Certificate of Analysis
Cantidad5 mg
Condiciones de envíoTemperatura ambiente
FuenteWheat germ
ConjugadoAlexa Fluor 594
Para utilizar con (aplicación)Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Cell Painting
FormularioLyophilized
RecombinanteNative
EspecieWheat
Unit Size5 mg
Contenido y almacenamiento
Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.
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Certificados

N.º de loteCertificate TypeDateCatalog Number(s)
3212492Certificate of Analysis17 jun 2025W56132
3029766Certificate of Analysis09 abr 2025W11261
3110690Certificate of Analysis30 mar 2025W32464
AC414186Certificate of Analysis27 mar 2025W56134
AB404930Certificate of Analysis06 mar 2025W56133
Se muestran 5 resultados, busque arriba un certificado específico

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Preguntas frecuentes

Yes. Although labeling in buffer (such as Hank's Buffered Saline Solution) is slightly better for brightness and lower non-cell background, media can be used. Do a concentration range to dertermine optimal conditions, since the WGA may potentially bind media components to some extent, slightly decreasing your specific labeling intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

No. For paraffin sections, there are few options due to the delipidation of the membranes by solvents used in the deparaffinization steps. The only good option is to use an antibody against a plasma membrane protein.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

No. Those lectins have been shown to label Golgi in fixed and permeabilized cultured cells, but the selectivity is cell type dependent. Usually you wind up with other structures labeling as well, such as endoplasmic reticulum. The only guaranteed way to specifically label Golgi in already fixed cells is to use an antibody for a Golgi-specific antigen.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Confirm that the tracer you are using crosslinks to proteins or has a primary amine for fixation-either a hydrazide, lysine fixable dextran, or a protein conjugate.
Use aldehyde-based fixatives to cross link the amines on the tracer.
Inject a larger amount or higher concentration of the tracer. Tracers are generally injected at 1-20% concentrations (10 mg/mL or higher).
Confirm that you are using the correct fluorescent filter for detection. You can perform a spot test by pipetting a small amount of the undiluted stock solution of the tracer onto a slide, then view under the filter you are using on your microscope. This will confirm if the tracer fluorescence can be detected and the fluorescent microscope filter is working properly.
Review tissue fixation and handling procedures to confirm if any reagents or processing procedures could be affecting the tracer.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Wheat germ agglutinin and cholera toxin conjugates have been used for retrograde tracing. They may have some anterograde tracing in some applications. A selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing/protein-conjugates.html).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (82)

Citations & References
Abstract
Essential role of protein kinase C delta in platelet signaling, alpha IIb beta 3 activation, and thromboxane A2 release.
Authors:Yacoub D, Théorêt JF, Villeneuve L, Abou-Saleh H, Mourad W, Allen BG, Merhi Y
Journal:J Biol Chem
PubMed ID:16895913
'The protein kinase C (PKC) family is an essential signaling mediator in platelet activation and aggregation. However, the relative importance of the major platelet PKC isoforms and their downstream effectors in platelet signaling and function remain unclear. Using isolated human platelets, we report that PKCdelta, but not PKCalpha or PKCbeta, ... More
Obligate multivalent recognition of cell surface tomoregulin following selection from a multivalent phage antibody library.
Authors:Heitner T, Satozawa N, McLean K, Vogel D, Cobb RR, Liu B, Mahmoudi M, Finster S, Larsen B, Zhu Y, Zhou H, Müller-Tiemann B, Monteclaro F, Zhao XY, Light DR
Journal:J Biomol Screen
PubMed ID:17092910
'A therapeutic antibody candidate (AT-19) isolated using multivalent phage display binds native tomoregulin (TR) as a mul-timer not as a monomer. This report raises the importance of screening and selecting phage antibodies on native antigen and reemphasizes the possibility that potentially valuable antibodies are discarded when a monomeric phage display ... More
N-glycans are direct determinants of CFTR folding and stability in secretory and endocytic membrane traffic.
Authors:Glozman R, Okiyoneda T, Mulvihill CM, Rini JM, Barriere H, Lukacs GL,
Journal:J Cell Biol
PubMed ID:19307599
'N-glycosylation, a common cotranslational modification, is thought to be critical for plasma membrane expression of glycoproteins by enhancing protein folding, trafficking, and stability through targeting them to the ER folding cycles via lectin-like chaperones. In this study, we show that N-glycans, specifically core glycans, enhance the productive folding and conformational ... More
Polarized insertion of new membrane from a cytoplasmic reservoir during cleavage of the Drosophila embryo.
Authors:Lecuit T, Wieschaus E
Journal:J Cell Biol
PubMed ID:10953008
'Cellularization of the Drosophila embryo is a specialized form of cytokinesis that results in the formation of a polarized epithelium. The mechanisms of membrane growth during cytokinesis are largely unknown. It is also unclear whether membrane growth and polarization represent distinct processes that occur simultaneously or whether growth of the ... More
Dysmorphic photoreceptors in a P23H mutant rhodopsin model of retinitis pigmentosa are metabolically active and capable of regenerating to reverse retinal degeneration.
Authors:Lee DC, Vazquez-Chona FR, Ferrell WD, Tam BM, Jones BW, Marc RE, Moritz OL,
Journal:J Neurosci
PubMed ID:22323724
'This study evaluated the capacity of Xenopus laevis retina to regenerate photoreceptor cells after cyclic light-mediated acute rod photoreceptor degeneration in a transgenic P23H mutant rhodopsin model of retinits pigmentosa. After discontinuation of cyclic light exposure, we monitored histologic progression of retinal regeneration over a 3 week recovery period. To ... More
82 total citations

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