碱性磷酸酶活性染料
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碱性磷酸酶活性染料
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Invitrogen™

碱性磷酸酶活性染料

碱性磷酸酶活性染料是一款干细胞成像产品，允许使用者对多能干细胞（PSC）进行区别性染色。该染料是碱性磷酸酶（alkaline phosphatase，AP）一种荧光底物，具有细胞渗透性，对细胞无毒性，两个小时即完成扩散。碱性磷酸酶活性染料由 DMSO 中的了解更多信息
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货号数量
A143531 管
货号 A14353
价格（CNY)
5,211.00
Each
下单后预计有货日期 04-Nov-2025
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1 管
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价格（CNY)
5,211.00
Each
添加至购物车
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碱性磷酸酶活性染料是一款干细胞成像产品，允许使用者对多能干细胞（PSC）进行区别性染色。该染料是碱性磷酸酶（alkaline phosphatase，AP）一种荧光底物，具有细胞渗透性，对细胞无毒性，两个小时即完成扩散。碱性磷酸酶活性染料由 DMSO 中的 50 µL 500X 荧光素染料组成，足够用于染色两块 24 孔板、四块 6 cm 培养皿或两块 10 cm 培养皿。

该碱性磷酸酶活性染料能够让您：

• 识别多能干细胞，具有高于背景荧光的强荧光信号
• 通常在 20 分钟或更短时间内就能完成 PSC 染色
• 使用第一种市售的能维持细胞活力的碱性磷酸酶染色剂

碱性磷酸酶多能干细胞检测方法
AP 是 PSC 的表型标志物，包括未分化的胚胎干细胞 (ESC)、诱导多能干细胞 (iPSC) 和胚胎生殖细胞 (EGC)。AP 在大多数细胞类型中都有表达，但在 PSC 中其表达量大幅增加。因此，AP 染色可用于区别性染色 PSC，以便轻松将其与用作饲养层的小鼠胚胎成纤维细胞 (MEF) 和通常用于重编程实验的亲代成纤维细胞进行区分。

在不牺牲珍贵的集落的情况下进行多能性筛选
碱性磷酸酶活性染料能够维持干细胞活力，是在重编程工作流程早期筛选集落的理想选择。它也可以在后期用作阴性选择工具，用于识别未分化细胞。

细胞快速染色
碱性磷酸酶活性染料使用一种简单、非永久性、保持细胞活力的方案来识别您的实验中的 PSC。只需将染料稀释在培养基中，涂抹于细胞，轻轻清洗，细胞即可用于荧光成像。

仅供研究使用。不可用于诊断程序。
仅供科研使用。不可用于诊断程序。
规格
细胞类型干细胞（iPS - 诱导型多能干细胞）、干细胞（胚胎）
检测方法荧光
染料类型经典染料
形式液体
数量1 管
研究类别分化
运输条件干冰
子细胞定位细胞膜&脂质
底物类型AP（碱性磷酸酶）底物
颜色绿色
适用于（应用）活力测定试剂盒
适用于（设备）Floid™ 细胞成像系统, 荧光显微镜
产品类型碱性磷酸酶活性染色剂
Unit SizeEach
内容与储存
包含 1 个琥珀色样品瓶。储存在 -20°C 下。
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常见问题解答 (FAQ)

我如何才能将iPSC可视化？

iPSC克隆可通过碱性磷酸酶染色法轻松实现可视化，相关试剂盒有碱性磷酸酶活细胞染色试剂盒（Alkaline Phosphatase Live Stain，货号 A14353）。此外，重编程克隆可通过Tra1-60或Tra1-81抗体的活细胞染色来进行筛选，这些抗体能够识别未分化的iPSC，进而帮助用户从各种人体细胞中鉴定出重编程细胞。请参考用户手册（https://tools.thermofisher.com/content/sfs/manuals/AP_Live_Stain_man.pdf）获取完整的实验方案。

How can I prevent poor or dim staining when using Alkaline Phosphatase Live Stain?

Serum or serum replacement components in the growth medium may cause background and this can result in poor or dim staining. After the removal of the growth medium, gently wash the culture with pre-warmed DMEM/F-12 (Cat. No. 10565-018) for 5 minutes. Aspirate and repeat once before adding the AP Live Stain. To further decrease background staining, perform 3 separate washes of 5 minutes each with DMEM/F-12 for a total of 15 minutes following the staining, and visualize immediately.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should Alkaline Phosphatase Live Stain be stored?

Store AP Live Stain at -20 degrees C in the freezer and thaw at room temperature. Avoid repeated freeze/thaw cycles and aliquot the AP Live Stain into smaller volumes if necessary. Always protect from light and avoid extended exposure at room temperature and atmospheric conditions.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use Alkaline Phosphatase Live Stain in my reprogramming experiments?

AP Live Stain is ideal for screening colonies during early stages of reprogramming since it selectively stains PSCs while maintaining cell viability. It can be used in later stages of reprogramming to identify undifferentiated cells for the selection of iPSCs for further cultivation. AP Live Stain was developed specifically for use on live cultures for cell maintenance and is qualified to be free of mycoplasma and bioburden, and exhibits extremely low endotoxin levels.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long should I wait before using Alkaline Phosphatase Live stain on my cells again?

Colonies may be restained as early as 24 hours after the initial staining. It is recommended that cells are allowed to recover properly to obtain consistent results.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all 碱性磷酸酶活性染料 FAQs

图表

AP Live Stain Workflow
AP Live Stain Workflow
Figure 2: Live AP Staining is reversible.
Figure 2: Live AP Staining is reversible.
Figure 3: Viability of cells following staining with AP Live Stain.
Figure 3: Viability of cells following staining with AP Live Stain.
Figure 1: Alkaline Phosphatase (AP) Live Stain shows robust staining in pluripotent stem with minimal background in fibroblasts.
Figure 1: Alkaline Phosphatase (AP) Live Stain shows robust staining in pluripotent stem with minimal background in fibroblasts.

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批号Certificate TypeDateCatalog Number(s)
2419886Certificate of Analysis2025年1月29日A14353
2577106Certificate of Analysis2025年1月29日A14353
2943079Certificate of Analysis2025年1月26日A14353
2191450Certificate of Analysis2021年1月18日A14353
2219977Certificate of Analysis2020年4月08日A14353
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引用和文献 (3)

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引用和文献
Abstract
Novel Live Alkaline Phosphatase Substrate for Identification of Pluripotent Stem Cells.
Authors:Singh U, Quintanilla RH, Grecian S, Gee KR, Rao MS, Lakshmipathy U,
Journal:Stem Cell Rev
PubMed ID:22426885
Characterization of pluripotent stem cells.
Authors:Martí M, Mulero L, Pardo C, Morera C, Carrió M, Laricchia-Robbio L, Esteban CR, Izpisua Belmonte JC,
Journal:Nat Protoc
PubMed ID:23306458
Characterization of pluripotent stem cells is required for the registration of stem cell lines and allows for an impartial and objective comparison of the results obtained when generating multiple lines. It is therefore crucial to establish specific, fast and reliable protocols to detect the hallmarks of pluripotency. Such protocols should ... More
Immune Defense Protein Expression in Highly Purified Mouse Lung Epithelial Cells.
Authors:Sinha M, Lowell CA
Journal:Am J Respir Cell Mol Biol
PubMed ID:26574781
Lung epithelial cells play critical roles in initiating and modulating immune responses during pulmonary infection or injury. To better understand the spectrum of immune response-related proteins present in lung epithelial cells, we developed an improved method of isolating highly pure primary murine alveolar type (AT) II cells and murine tracheal ... More
3 total citations

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