Ampicilina, sal sódica, irradiada
Ampicilina, sal sódica, irradiada
Gibco™

Ampicilina, sal sódica, irradiada

La ampicilina es un antibiótico del grupo de la penicilina de amplio espectro. La ampicilina se distingue de la penicilinaMás información
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Número de catálogoCantidad
11593027200 mg
Número de catálogo 11593027
Precio (EUR)
69,00
Each
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Cantidad:
200 mg
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Precio (EUR)
69,00
Each
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Ask our AI about this Product
La ampicilina es un antibiótico del grupo de la penicilina de amplio espectro. La ampicilina se distingue de la penicilina tan solo por la presencia de un grupo amino que facilita la penetración a través de la membrana externa de algunas bacterias gramnegativas. La ampicilina actúa interfiriendo directamente con el volumen de la pared celular bacteriana, e indirectamente mediante la activación de la liberación de enzimas que alteran aún más la pared celular. La ampicilina Gibco™ se emplea como un antibiótico selectivo para bacterias resistentes, generalmente con una concentración de 10-25 μg/ml para medios líquidos, y 35-50 μg/ml para placas. Este producto se suministra en forma de polvo y se debe convertir en una solución madre de 10 mg/ml en agua. La ampicilina no se utiliza para prevenir la contaminación bacteriana de los cultivos celulares debido a preocupaciones relativas a la toxicidad en células de mamíferos e insectos. Ofrecemos una gran variedad de antibióticos de cultivo celular entre los que podrá elegir.

Uso del producto
Para uso exclusivo en investigación: no diseñado para uso diagnóstico o terapéutico en animales o humanos.

Fabricación conforme con las buenas prácticas de fabricación actuales
La ampicilina Gibco™ se fabrica en una instalación conforme con las buenas prácticas de fabricación actuales, ubicada en Grand Island, Nueva York (EE. UU.). Las instalaciones están registradas en la FDA como fabricante de dispositivos médicos y están certificadas según la norma ISO 13485.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ConcentraciónDe 20 a 125 µg/ml
Para utilizar con (aplicación)Selección bacteriana
Cantidad200 mg
Duración de almacenamiento24 meses
Condiciones de envíoTemperatura ambiente
FormularioPolvo
Tipo de productoAmpicilina
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 a 8 °C
Condiciones de envío: Ambiente
Vida útil: 24 meses a partir de la fecha de fabricación
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Certificados

N.º de loteCertificate TypeDateCatalog Number(s)
2588011Certificate of Analysis26 ene 202511593027
2913062Certificate of Analysis25 ene 202511593027
A0330966Certificate of Analysis16 ago 202111593027
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Hojas de datos de seguridad

Preguntas frecuentes

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

No. B-lactamase is targeted to specific linkages in the bacterial cell wall. Since eukaryotic cells lack a cell wall, ampicillin has no effect upon eukaryotic cells.

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (7)

Citations & References
Abstract
Human GBP1 is a microbe-specific gatekeeper of macrophage apoptosis and pyroptosis.
Authors:Fisch D, Bando H, Clough B, Hornung V, Yamamoto M, Shenoy AR, Frickel EM
Journal:EMBO J
PubMed ID:31268602
'The guanylate binding protein (GBP) family of interferon-inducible GTPases promotes antimicrobial immunity and cell death. During bacterial infection, multiple mouse Gbps, human GBP2, and GBP5 support the activation of caspase-1-containing inflammasome complexes or caspase-4 which trigger pyroptosis. Whether GBPs regulate other forms of cell death is not known. The apicomplexan ... More
Id1 Ablation Protects Hematopoietic Stem Cells from Stress-Induced Exhaustion and Aging.
Authors:Singh SK, Singh S, Gadomski S, Sun L, Pfannenstein A, Magidson V, Chen X, Kozlov S, Tessarollo L, Klarmann KD, Keller JR
Journal:Cell Stem Cell
PubMed ID:30082068
'Defining mechanisms that maintain tissue stem cells during homeostasis, stress, and aging is important for improving tissue regeneration and repair and enhancing cancer therapies. Here, we show that Id1 is induced in hematopoietic stem cells (HSCs) by cytokines that promote HSC proliferation and differentiation, suggesting that it functions in stress ... More
Generation of Mutants of Nuclear-Encoded Plastid Proteins Using CRISPR/Cas9 in the Diatom Phaeodactylum tricornutum.
Authors:Allorent G, Guglielmino E, Giustini C, Courtois F
Journal:Methods Mol Biol
PubMed ID:29987734
Genome modifications in microalgae are becoming a widespread and mandatory tool for research in both fundamental and applied biology. Among genome editing methods in these photosynthetic organisms, CRISPR/Cas9 offers a specific, powerful and efficient tool for genome engineering by inducing mutations in targeted regions of the genome. Here we described ... More
Completeness of HIV-1 Envelope Glycan Shield at Transmission Determines Neutralization Breadth.
Authors:Wagh K, Kreider EF, Li Y, Barbian HJ, Learn GH, Giorgi E, Hraber PT, Decker TG, Smith AG, Gondim MV, Gillis L, Wandzilak J, Chuang GY, Rawi R, Cai F, Pellegrino P, Williams I, Overbaugh J, Gao F, Kwong PD, Haynes BF, Shaw GM, Borrow P, Seaman MS, Hahn BH, Korber B
Journal:Cell Rep
PubMed ID:30355496
Densely arranged N-linked glycans shield the HIV-1 envelope (Env) trimer from antibody recognition. Strain-specific breaches in this shield (glycan holes) can be targets of vaccine-induced neutralizing antibodies that lack breadth. To understand the interplay between glycan holes and neutralization breadth in HIV-1 infection, we developed a sequence- and structure-based approach ... More
Cannabidiol directly targets mitochondria and disturbs calcium homeostasis in acute lymphoblastic leukemia.
Authors:Olivas-Aguirre M, Torres-López L, Valle-Reyes JS, Hernández-Cruz A, Pottosin I, Dobrovinskaya O
Journal:Cell Death Dis
PubMed ID:31611561
Anticancer properties of non-psychoactive cannabinoid cannabidiol (CBD) have been demonstrated on tumors of different histogenesis. Different molecular targets for CBD were proposed, including cannabinoid receptors and some plasma membrane ion channels. Here we have shown that cell lines derived from acute lymphoblastic leukemia of T lineage (T-ALL), but not resting ... More
7 total citations

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