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Reactivo SYTOX™ Green Ready Flow™
Reactivo SYTOX™ Green Ready Flow™
Invitrogen™

Reactivo SYTOX™ Green Ready Flow™

El reactivo SYTOX™ Green Ready Flow™ es una tinción de ácido nucleico brillante y fácil de usar para distinguir lasMás información
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Código de descuento:RFLOW25
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Número de catálogoCantidad
R37168Promo Image120 ensayos
Número de catálogo R37168
Precio (EUR)
278,65
Online Exclusive
371,00
Ahorro 92,35 (25%)
Each
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Cantidad:
120 ensayos
Recurring order eligible. Learn more »
Precio (EUR)
278,65
Online Exclusive
371,00
Ahorro 92,35 (25%)
Each
Añadir al carro de la compra
Ask our AI about this Product
El reactivo SYTOX™ Green Ready Flow™ es una tinción de ácido nucleico brillante y fácil de usar para distinguir las células muertas de las vivas en ensayos de citometría de flujo. Es excitable a 488 nm con un perfil de emisión similar a la adquisición de imágenes de células etiquetadas con fluoresceína (FITC) y se ha probado en varias líneas de células.

• Estable a temperatura ambiente
• Formato cómodo y listo para su uso sin necesidad de diluir, pesar o pipetear
• Fluorescencia verde excepcionalmente brillante al unirse a ácidos nucleicos
• Tinción rápida de células apoptóticas sin pasos de lavado

El reactivo de SYTOX Green Ready Flow es una tinción de células muertas lista para su uso, directa a la muestra. Añada dos gotas del frasco cuentagotas directamente a 1 x 106 células, incube y analice.

Como colorante impermeable a la célula, SYTOX Green entra en la célula tras la pérdida de la integridad de la membrana y se une al ADN, actuando así como contratinción que se puede analizar cuando se excita a 488 nm y la emisión se captura a un pico de 523 nm. El reactivo SYTOX Green se puede utilizar tanto en células de mamíferos como bacterianas y a menudo se incluye en ensayos de multiplexado.

Especificaciones
Permeabilidad celularImpermeable
DescripciónReactivo SYTOX™ Green Ready Flow™
Método de detecciónFluorescente
Tipo de coloranteSYTOX™ Green Ready Flow™
FormularioSolución
FormatoFrasco(s)
Cantidad120 ensayos
SolubilidadDMSO (dimetilsulfóxido)
ColorVerde
Emission523 nm
Excitation Wavelength Range504 nm
Para utilizar con (aplicación)Ensayo de viabilidad
Para utilizar con (equipo)Citómetro de flujo
Línea de productosReady Flow™, Sytox™
Tipo de productoReactivo
Unit SizeEach
Contenido y almacenamiento
Incluye 2 frascos cuentagotas, almacenar a temperatura ambiente.
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Preguntas frecuentes

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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Certificados

N.º de loteCertificate TypeDateCatalog Number(s)
3182983Certificate of Analysis04 may 2025R37168
3143968Certificate of Analysis17 mar 2025R37168
2980694Certificate of Analysis24 sept 2024R37168
2800329Certificate of Analysis14 mar 2024R37168
2714535Certificate of Analysis13 sept 2023R37168
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