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Ion AmpliSeq™ Direct FFPE DNA Kit
Ion AmpliSeq™ Direct FFPE DNA Kit
Ion Torrent™

Ion AmpliSeq™ Direct FFPE DNA Kit

The Ion AmpliSeq™ Direct FFPE DNA Kit enables the preparation of DNA from formalin-fixed, paraffin-embedded (FFPE) tissues for library constructionAfficher plus
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RéférenceNbre de réactions
A311338 Preps
A3113696 Preps
Référence A31133
Prix (EUR)
97,25
Each
Délai de disponibilité estimé 29-Jul-2025
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Nbre de réactions:
8 Preps
Recurring order eligible. Learn more »
Prix (EUR)
97,25
Each
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Ask our AI about this Product
The Ion AmpliSeq™ Direct FFPE DNA Kit enables the preparation of DNA from formalin-fixed, paraffin-embedded (FFPE) tissues for library construction using the Ion AmpliSeq protocol, without the need for deparaffinization or DNA purification. This kit is suitable for FFPE tissue sections up to 10 μm thick.

Features of the Ion AmpliSeq Direct FFPE DNA Kit include:
• Simple one-tube 2-step protocol that minimizes sample loss
• Efficient 30-minute workflow with only 10 minutes of hands-on time
• Ideal for FFPE DNA samples with limited DNA amount
• No deparaffinization of FFPE sections required
• No column or bead-based DNA purification required
• Ion AmpliSeq library preparation with as little as 1 ng input DNA
• Uracil-D-glycosylase treatment to remove deaminated cytosines is an optional step

Extraction of DNA from samples with limited DNA amount
Archived tissue samples can provide clinically relevant information, but it can be difficult to isolate the small amount of nucleic acid they contain. Deparaffinization of FFPE tissue sections and purification of the DNA in these samples can be a complicated, multi-step procedure requiring considerable hands-on time, and the associated sample-loss makes it an unsuitable approach for processing FFPE DNA samples with limited DNA amounts.

A simpler and faster method
The Ion AmpliSeq Direct FFPE DNA Kit provides a user-friendly protocol that eliminates the need for deparaffinization of FFPE tissue sections and purification of the DNA. From an FFPE section mounted on a glass slide, the area of interest is removed using Transfer Solution and pipetted into a PCR tube or 96-well plate. After the addition of Direct Reagent, the sample is incubated at 65°C for 15 minutes. The FFPE DNA is now ready for the Ion AmpliSeq™ Library Preparation protocol. The prepared FFPE DNA can be quantified using the Qubit™ HS DNA Quantitation Kit, if necessary. The simplicity of this protocol eliminates the need for complicated deparaffinization and DNA extraction procedures, and libraries can be prepared either manually or with the automated Ion Chef™ System.

For Research Use Only. Not for use in diagnostic procedures.
Spécifications
FormatKit
À utiliser avec (application)Next-Generation Sequencing
Compatibilité à haut débitHigh-throughput Compatible
Nbre de réactions8 Preps
Gamme de produitsAmpliSeq™
Type de produitDirect FFPE DNA Kit
Quantité8 preps
Conditions d’expéditionGlace
CibleADN génomique
Unit SizeEach
Contenu et stockage
• 240 μL Transfer Solution
• 170 μL Direct Reagent

Store at 2–8°C.
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      Documentation et téléchargements

      Certificats

      Numéro de lotCertificate TypeDateCatalog Number(s)
      3255088Certificate of Analysis05 juin 2025A31136
      3235922Certificate of Analysis09 mai 2025A31133
      3180493Certificate of Analysis28 févr. 2025A31136
      3140903Certificate of Analysis07 janv. 2025A31133
      3140896Certificate of Analysis07 janv. 2025A31133
      5 résultats affichés, recherchez un certificat spécifique ci-dessus

      Safety Data Sheets

      Foire aux questions (FAQ)

      We recommend the following DNA isolation kits:

      - RecoverAll Total Nucleic Acid Isolation Kit for FFPE (Cat. No. AM1975)
      - Ion Ampliseq Direct FFPE DNA Kit (Cat. Nos. A31133, A31136)
      - MagMAX FFPE DNA/RNA Ultra Kit (Cat. No. A31881)
      - PureLink Genomic DNA Mini Kit (Cat. Nos, K182000, K182001, K182002)

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