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pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli
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Invitrogen™

pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ TOP10 E. coli

The pCR™8/GW/TOPO™ TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway™ recombination cloning vector platform. OurRead more
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Catalog NumberQuantity
K25002020 Reactions
Catalog number K250020
Price (HKD)
4,440.00
Online Exclusive
Ends: 31-Dec-2025
5,332.00
Save 892.00 (17%)
Each
Add to cart
Quantity:
20 Reactions
Recurring order eligible. Learn more »
Price (HKD)
4,440.00
Online Exclusive
Ends: 31-Dec-2025
5,332.00
Save 892.00 (17%)
Each
Add to cart
Ask our AI about this Product
The pCR™8/GW/TOPO™ TA Cloning Kit delivers simple 5 minute cloning and access to the Gateway™ recombination cloning vector platform. Our pCR™8 TOPO™ TA vector contains a minimal MCS, so you sequence more of your insert and less of the vector.

With our patented att sites, the pCR™8 vector is the simplest way to create an Entry Clone, which facilitates easy access via Gateway™ recombination cloning to a variety of expression vectors without tedious sub-cloning, saving you valuable research time.

The pCR™8/GW/TOPO™ TA Cloning Kit offers:

att sites for rapid recombination into a variety of Gateway™ destination vectors
• 3´-T overhangs for direct ligation of Taq-amplified PCR products
• Sequencing primer sites within 55 bp of insertion site ensures you sequence more insert, less vector
• Spectinomycin resistance gene for robust selection in E. coli
• EcoRI sites flanking the PCR product insertion site for easy excision of inserts

This kit includes One Shot™ TOP10 Chemically Competent E. coli.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cloning MethodTOPO™ with Gateway™
No. of Reactions20 reactions
Product LineOne Shot™
Product TypeTA Cloning Kit
Quantity20 Reactions
VectorpCR
Unit SizeEach
Contents & Storage
The pCR™8/GW/TOPO™ TA Cloning Kit contains the TOPO™-adapted, ready-to-use pCR™8/GW/TOPO™ Vector, dNTPs, Salt Solution, Sterile Water, Gateway Forward and Reverse Sequencing Primers, Control Template and PCR Primers, One Shot™ TOP10 Chemically Competent E. coli Chemically Competent E. coli, S.O.C. Medium, and a pUC19 control plasmid. Store the One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored.
Ready-to-use Bacterial Growth Media

Ready-to-use Bacterial Growth Media

See how these mediums can help with critical aspects of cloning!

Gibco LB Broth

Invitrogen S.O.C. Medium

Invitrogen One Shot LB Agar*

*Only available in North America and selected European countries

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Vector Information

Vector Name
Vector Map
Polylinker
Sequence
Restriction
pCR8/GW/TOPO

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