What should I do about the colloidal carbon (black precipitate) in SuperBlock Blocking Buffer solution?
Allow any colloidal carbon, if present, to settle to the bottom of the bottle and pouring off what you need to use. We do not recommend centrifugation or filtering because these procedures may reduce the product's blocking capabilities.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
What preservative is used in SuperBlock Blocking Buffer, and what is its source?
Kathon Preservative, a broad spectrum antimicrobial agent, is used at a concentration of 600 ppm.
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What incubation time and temperature should I use for effective blocking using the SuperBlock Blocking Buffer ?
Typical procedures use 1 hour at room temperature or overnight at 4°C. However, sufficient blocking in many procedures is possible in 3 x 2 minutes at room temperature.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.