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推奨事項
The BigDye™ Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.
- Improve the quality of your results for a wide range of sequencing applications
- Optimized for long read lengths
- Better dye mobility characteristics
- Improved performance reading through GT-rich regions
- Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
- Enhance your productivity and reduce costs
Get more uniform peak heights and improved accuracy
With BigDye™ Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.
For Research Use Only. Not for use in diagnostic procedures.
仕様
フォーマット
12-well Strips, 8-well Strip, 96- or 384-well Plate
使用対象 (装置)
SeqStudio™ Genetic Analyzer, SeqStudio™ Flex Series Genetic Analyzer, 3500/3500xL Genetic Analyzer, 3730/3730xl DNA Analyzer Thermal Cyclers: GeneAmp™ PCR System 9700 Dual Series, Veriti™ Fast 96‐well and 384‐well Thermal Cyclers
使用対象（アプリケーション）
Standard Sequencing
製品ライン
BigDye™ Terminator
製品タイプ
Sequencing Buffer
数量
25,000 Reactions
技術
Fluorescent Dye Terminator Sequencing
テンプレート適合性
BAC DNA, Plasmid DNA (≤15Kb), PCR Amplicons, Single Stranded DNA
組成および保存条件
• 10 x 20 mL tubes of BigDye™ Terminator v3.1 Ready Reaction Mix
• 4 tubes M13 (-21) Primer
• 4 tubes pREF-BDT™ Control DNA
• 1 x 233 mL tube of 5X Sequencing Buffer (may be stored at 4°C)
Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.
• 4 tubes M13 (-21) Primer
• 4 tubes pREF-BDT™ Control DNA
• 1 x 233 mL tube of 5X Sequencing Buffer (may be stored at 4°C)
Store kit at -15°C to -25°C, and avoid repeated freeze-thaw cycles.
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