MicroSEQ™ 500 16S rDNA Sequencing Kit
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Applied Biosystems™

MicroSEQ™ 500 16S rDNA Sequencing Kit

The MicroSEQ™ 500 16S rDNA Sequencing Kit is the sequencing component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System,詳細を見る
製品番号(カタログ番号)数量
43464801キット
製品番号(カタログ番号) 4346480
価格(JPY)
47,200
Each
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数量:
1キット
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The MicroSEQ™ 500 16S rDNA Sequencing Kit is the sequencing component of the MicroSEQ™ 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria. Use this kit to sequence PCR products that have been generated using the MicroSEQ™ 500 16S rDNA PCR Kit. The two kits together permit the quick and convenient amplification and sequencing of 500 base pairs of the 16S rRNA gene. Sequences can then be compared to the MicroSEQ™ 16S rDNA 500 Library to generate an identity match. This kit does not include a protocol or a Quick Reference Card.

• Sequence-based measurement ensures you get reproducible results in under 24 hours.
• Growth requirement-independent measurement enables you to identify bacteria using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ™ ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training-same work flow as the MicroSEQ™ D2 LSU fungal kit.

The MicroSEQ™ ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems™ MicroSEQ™ System combines the advantages of MicroSEQ™ ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSeq bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

For Research Use Only. Not for use in diagnostics procedures.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
検出法Capillary Electrophoresis
使用対象(アプリケーション)配列
使用対象 (装置)310 Genetic Analyzer, 3100 Genetic Analyzer, 3100-Avant Genetic Analyzer, 3130xl Genetic Analyzer, 3730xl DNA Analyzer, GeneAmp 9700, 3730 DNA Analyzer, 3500xL Genetic Analyzer, 3500 Genetic Analyzer
内容Forward sequencing mix (2 tubes), and reverse sequencing mix (2 tubes)
ライブラリCustomizable, Bacterial Library (> 2000 species)
反応数55 Forward, 55 Reverse
製品ラインMicroSEQ™
製品タイプSequencing Kit
数量1キット
サンプルタイプPCR Products
出荷条件Dry Ice
ソフトウェアMicroSEQ™ ID Analysis Software
ターゲット1st 500 bp of 16S rRNA gene
対象微生物クラスBacteria
技術SBT (Sequence-Based Typing)
ワークフローステップCycle Sequencing
フォーマットキット
Unit SizeEach
組成および保存条件
Each kit contains:
Two tubes each of forward and reverse sequencing mix. Sufficient reagent to perform 55 forward and 55 reverse sequencing reactions. Store at -15 to -25°C.
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証明書

ロット番号Certificate TypeDateCatalog Number(s)
2505222Certificate of Analysis2025年6月14日4346480
2503221Certificate of Analysis2025年3月26日4346480
2501220Certificate of Analysis2025年3月16日4346480
2411219Certificate of Analysis2024年12月13日4346480
2410218Certificate of Analysis2024年11月05日4346480
5件の結果が表示されました。 上記から特定の証明書を検索します

Safety Data Sheets

LULL (Limited Use of Label License)

'

Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non-transferable right to use the purchased amount of the product (a) to perform internal research for the sole benefit of the purchaser; and (b) for environmental testing, quality control/quality assurance testing, food and agricultural testing, including reporting results of purchaser's activities in environmental testing, quality control/quality assurance testing, food and agricultural testing for a fee or other commercial consideration. No other right is hereby granted expressly, by implication, or by estoppel. This product is for environmental testing, quality control/ quality assurance testing, food and agricultural testing and research purposes only. The purchase of this product does not grant the purchaser any additional rights, including (without limitation) the right to transfer or resell the product in any form or the right to use the product as a therapeutic agent or diagnostics test component. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.

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よくあるご質問(FAQ)

To take advantage of the reduced amplification and sequencing times allowed by the new fast PCR chemistry and the 9800 Fast Thermal Cycler, we recommend using the Applied Biosystems 9800 Fast Thermal Cycler with the MicroSEQ kits. The GeneAmp PCR System 9700 may also be used but note that the 9700 thermocycler does not provide the reduced amplification and sequencing times allowed by the new fast PCR chemistry and the 9800 Fast Thermal Cycler.

The MicroSEQ 500 16S rDNA Sequencing Kit is stable if stored at -20 degrees C for 15-16 months. We do not have any information available for temperatures outside of this range.

Find additional tips, troubleshooting help, and resources within our Capillary Electrophoresis Applications Support Center.

No, it is for RUO.

引用および参考文献 (159)

引用および参考文献
Abstract
Structures of aldouronic acids liberated from kenaf xylan by endoxylanases from Streptomyces sp.
Authors:Komiyama, H; Enomoto, A; Sueyoshi, Y; Nishio, T; Kato, A; Ishii, T; Shimizu, K
Journal:
PubMed ID:
'Streptomyces sp. SK519 was cultivated in the medium containing 0.5% birch wood xylan to produce extracellular xylanase. The xylanase was isolated from the culture supernatant by ammonium sulfate precipitation and partially purified by absorbing on the insoluble xylan. Alkali-extracted kenaf glucuronoylan of which main chain primarily consisting of (1-4)-linked β-D-xylopyranose ... More
Pseudomonas panipatensis sp. nov., isolated from an oil-contaminated site
Authors:Gupta, SK; Kumari, R; Prakash, O; Lal, R
Journal:
PubMed ID:
'A Gram-negative, motile, rod-shaped, non-sporulating, aerobic bacterial strain (Esp-1(T)) was isolated from oil-contaminated soil of Panipat Oil Refinery, India, and its taxonomic position was determined using a polyphasic approach. Strain Esp-1(T) grew in the presence of 2% NaCl at 30 °C and was characterized chemotaxonomically by having C(16:0) as the ... More
Multilocus DNA sequence comparisons rapidly identify pathogenic molds.
Authors:Rakeman JL; Bui U; Lafe K; Chen YC; Honeycutt RJ; Cookson BT
Journal:
PubMed ID:
'The increasing incidence of opportunistic fungal infections necessitates rapid and accurate identification of the associated fungi to facilitate optimal patient treatment. Traditional phenotype-based identification methods utilized in clinical laboratories rely on the production and recognition of reproductive structures, making identification difficult or impossible when these structures are not observed. We ... More
Species distribution and antimicrobial resistance of enterococci isolated from surface and ocean water
Authors:Moore, DF; Guzman, JA; Mcgee, C
Journal:
PubMed ID:
'Aims: The species identification and antimicrobial resistance profiles were determined for enterococci isolated from Southern California surface and ocean waters. Methods and Results: Species identification was determined for 1413 presumptive Enterococcus isolates from urban runoff, bay, ocean and sewage water samples. The most frequently isolated species were Enterococcus faecalis, Enterococcus ... More
Multicenter evaluation of the new VITEK 2 advanced colorimetric yeast identification card.
Authors:Hata DJ; Hall L; Fothergill AW; Larone DH; Wengenack NL
Journal:
PubMed ID:
'The performance of the new VITEK 2 Advanced Colorimetry yeast identification (YST) card for use with the VITEK 2 system (bioMerieux, Inc., Hazelwood, MO) was compared to that of the API 20C AUX (API) system (bioMerieux SA, Marcy-l''Etoile, France) in a multicenter evaluation. A total of 12 quality control, 64 ... More
159 total citations

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