The Gibco AAV-MAX Transfection Kit is an essential component of the AAV-MAX Helper-Free AAV Production System. It is formulated for the transient transfection of Viral Production Cells 2.0. The kit includes a cationic lipid-based transfection reagent, as well as a transfection booster and production enhancer, which are designed to work together to produce adeno-associated virus (AAV) particles from Viral Production Cells 2.0 when cultured in Viral Production Medium.
Features of the AAV-MAX Transfection Kit include: • Components work synergistically within the AAV-MAX system to produce high AAV titers greater than 5 x 1010 vg/mL (unpurified) • AAV-MAX transfection reagent—cationic lipid-based transfection reagent for helper virus-free triple transfection of high-density cultures • AAV-MAX booster—chemically defined component that works with transfection reagent to achieve high transfection efficiency • AAV-MAX enhancer—chemically defined component that enhances the production of AAV particles • Robust transfection protocols are adaptable to large scale transfection
For Research Use Only. Not for use in diagnostic procedures.
仕様
細胞株
HEK293
培養タイプ
Suspension Cell Culture
概要
Serum-free, animal origin-free, chemically defined
細胞タイプ
Established Cell Lines
フォーマット
Bottle
サンプルタイプ
Plasmid DNA
Transfection Technique
Lipid-based Transfection
発現システム
Mammalian
使用対象 (装置)
AAV-MAX System
使用対象(アプリケーション)
AAV Production
内容
10 mL Gibco AAV-MAX Enhancer, 6 mL Gibco AAV-MAX Transfection Reagent, 3 mL Gibco AAV-MAX Supplement
製品ライン
AAV-MAX
製品タイプ
AAV production transfection Kit
数量
1 kit for 1 L of production
試薬タイプ
Transfection kit
品質保持期間
12 months from date of manufacture
出荷条件
Wet Ice
無菌性
Sterile
Unit Size
For 1 L of culture
組成および保存条件
• 6 mL AAV-MAX Trasfection Reagent • 3 mL AAV-MAX Transfection Booster • 10 mL AAV-MAX Enhancer
Reagents should be stored at 2–8°C. Do not freeze.