BrdU (5-Bromo-2'-Deoxyuridine)
BrdU (5-Bromo-2'-Deoxyuridine)
Invitrogen™

BrdU (5-Bromo-2'-Deoxyuridine)

細胞は、細胞分裂中にチミジン類似体であるBrdUを自然に取り込むことができるため、このヌクレオチド類似体は細胞周期および増殖の優れたマーカーとなります。取り込まれたBrdUは、抗BrdU抗体を使用して分析できます詳細を見る
製品番号(カタログ番号)数量
B23151100 mg
製品番号(カタログ番号) B23151
価格(JPY)
30,700
Each
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数量:
100 mg
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細胞は、細胞分裂中にチミジン類似体であるBrdUを自然に取り込むことができるため、このヌクレオチド類似体は細胞周期および増殖の優れたマーカーとなります。取り込まれたBrdUは、抗BrdU抗体を使用して分析できます。
研究用にのみ使用できます。診断用には使用いただけません。
仕様
標識または色素BrdU
製品タイプBrdU
数量100 mg
出荷条件室温
Unit SizeEach
組成および保存条件
フリーザー(-5℃~-30℃)に保存し、遮光してください。
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よくあるご質問(FAQ)

Can I combine EdU and BrdU labeling and detection on the same sample?

Yes, EdU and BrdU labeling can be combined for dual-pulse labeling of cell proliferation in cultured cells and in vivo. BrdU will be preferentially incorporated into DNA, so perform the EdU incubation first followed by the BrdU incubation. Removal of EdU from the media is not required in cultured cells when BrdU is added as the second label. Perform an alcohol fixation followed by some method of DNA denaturation as required for the BrdU detection protocol and then perform the click labeling reaction for detection of EdU followed by antibody labeling for detection of BrdU. Be sure to select a BrdU antibody that does not have cross-reactivity to EdU, such as our MoBU-1 clone (Cat. No. B35141). Many BrdU antibodies have been shown to have some amount of cross-reactivity with incorporated EdU. Here is a link (http://www.thermofisher.com/us/en/home/references/protocols/cell-and-tissue-analysis/flow-cytometry-protocol/cell-proliferation/dual-pulse-labeling-of-cell-proliferation-using-edu-and-brdu-incorporation.html) to an example protocol for dual-pulse labeling using EdU and BrdU.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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証明書

ロット番号Certificate TypeDateCatalog Number(s)
3192589Certificate of Analysis2025年7月13日B23151
3136422Certificate of Analysis2025年4月10日B23151
3135758Certificate of Analysis2025年4月09日B23151
2984246Certificate of Analysis2024年10月16日B23151
913310Certificate of Analysis2024年10月14日B23151
5件の結果が表示されました。 上記から特定の証明書を検索します

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引用および参考文献 (46)

引用および参考文献
Abstract
Detection of 5-bromo-2-deoxyuridine (BrdUrd) incorporation with monoclonal anti-BrdUrd antibody after deoxyribonuclease treatment.
Authors:Takagi S, McFadden ML, Humphreys RE, Woda BA, Sairenji T
Journal:Cytometry
PubMed ID:8404370
We studied the effects of deoxyribonucleases on the detection of 5-bromo-2-deoxyuridine (BrdUrd) by anti-BrdUrd monoclonal antibodies (mAbs). After DNase I treatment, BrdUrd was detected in cells fixed on slides with the anti-BrdUrd mAbs, B44 and BMC9318. The level of detection related to the degree of DNA digestion. DNA digestion of ... More
Rac1 inhibits myogenic differentiation by preventing the complete withdrawal of myoblasts from the cell cycle.
Authors:Heller H, Gredinger E, Bengal E
Journal:J Biol Chem
PubMed ID:11489882
'The small GTPase protein Rac1 is involved in a wide range of biological processes, yet its role in cell differentiation is mostly unknown. Here we show that Rac1 activity is high in proliferating myoblasts and decreases during the differentiation process. To analyze the involvement of Rac1 in muscle differentiation, different ... More
Androgen-stimulated DNA synthesis and cytoskeletal changes in fibroblasts by a nontranscriptional receptor action.
Authors:Castoria G, Lombardi M, Barone MV, Bilancio A, Di Domenico M, Bottero D, Vitale F, Migliaccio A, Auricchio F
Journal:J Cell Biol
PubMed ID:12743104
'In NIH3T3 cells, 0.001 nM of the synthetic androgen R1881 induces and stimulates association of androgen receptor (AR) with Src and phosphatidylinositol 3-kinase (Pl3-kinase), respectively, thereby triggering S-phase entry. 10 nM R1881 stimulates Rac activity and membrane ruffling in the absence of the receptor-Src-PI3-kinase complex assembly. The antiandrogen Casodex and ... More
Modulation of acto-myosin contractility in skeletal muscle myoblasts uncouples growth arrest from differentiation.
Authors:Dhawan J, Helfman DM
Journal:J Cell Sci
PubMed ID:15252113
'Cell-substratum interactions trigger key signaling pathways that modulate growth control and tissue-specific gene expression. We have previously shown that abolishing adhesive interactions by suspension culture results in G(0) arrest of myoblasts. We report that blocking intracellular transmission of adhesion-dependent signals in adherent cells mimics the absence of adhesive contacts. We ... More
Visualization of replication initiation and elongation in Drosophila.
Authors:Claycomb JM, MacAlpine DM, Evans JG, Bell SP, Orr-Weaver TL
Journal:J Cell Biol
PubMed ID:12403810
'Chorion gene amplification in the ovaries of Drosophila melanogaster is a powerful system for the study of metazoan DNA replication in vivo. Using a combination of high-resolution confocal and deconvolution microscopy and quantitative realtime PCR, we found that initiation and elongation occur during separate developmental stages, thus permitting analysis of ... More
46 total citations

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