Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL

You may remove excess solvent and smaller moieties by centrifugation through a microconcentrator. This will concentrate your protein sample.

(1) Choose microconcentrator tube (available from a variety of commercial suppliers) with a protein cutoff smaller than the molecular weight of the protein in the sample. Check our Pierce Protein Concentrators PES.

(2) Add 1 µL of 20% w/v SDS to a dry microconcentrator tube (if sample does not already contain SDS).

(3) Slowly add sample (a few microliters at a time) to membrane until membrane is completely wet. Centrifuge to near (but not complete) dryness.

(4) If intention is to desalt sample or buffer exchange: Add ~50 µL water to microconcentrator and spin until nearly dry. Repeat buffer exchange. Sample will remain on membrane. Check our Zeba desalting proteins.

(5) Using a new collection tube, invert membrane and spin at low speed (1000 x g) to elute protein from membrane. Add 2X SDS-Sample Buffer containing 10 mM DTT to membrane: vortex, invert and spin. Final volume should be ~20 µL.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

We recommend using Zeba Spin Desalting Columns, Plates, and Cartridges (Cat. No. 89882, 89883) to remove sodium azide from the Alix Monoclonal Antibody (3A9) storage solution.

Zeba Spin Desalting columns are covered under our general 1-year warranty and are guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Glycerol and sugars add viscosity to a sample, while detergents create micelles, making the removal of each difficult. Although dialysis is a better choice for removing these components, Zeba Columns can be used by modifying the desalting protocol. Modifications include diluting the samples, processing smaller samples, and adding the sample slowly to the column allowing it to enter the gel and equilibrate into the pores before centrifugation.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

You can either reduce the volume of sample processed in each column (the product manual indicates the volume range recommended for each column size) or run the flow-through from one column over a fresh column.