SYTOX™ Green Ready Flow™ Reagent
SYTOX™ Green Ready Flow™ Reagent
Invitrogen™

SYTOX™ Green Ready Flow™ Reagent

SYTOX™ Green Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in자세히 알아보기
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프로모션 코드:RFLOW25
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카탈로그 번호수량
R37168Promo Image120 Assays
카탈로그 번호 R37168
제품 가격(KRW)
457,000
Online offer
Ends: 30-Sep-2025
537,000
할인액 80,000 (15%)
Each
재고 있음
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수량:
120 Assays
Recurring order eligible. Learn more »
제품 가격(KRW)
457,000
Online offer
Ends: 30-Sep-2025
537,000
할인액 80,000 (15%)
Each
카트에 추가하기
Ask our AI about this Product
SYTOX™ Green Ready Flow™ Reagent is a bright, easy-to-use nucleic acid stain for distinguishing dead cells from live cells in flow cytometry assays. It is excitable at 488 nm with an emission profile similar to FITC, and has been tested in multiple cell lines.

• Stable at room temperature
• Convenient, ready-to-use format—no need to dilute, weigh, or pipette
• Exceptionally bright green fluorescence upon binding to nucleic acids
• Rapid staining without wash steps

SYTOX Green Ready Flow Reagent is a ready-to-use, direct-to-sample dead cell stain. Add 2 drops from the dropper bottle directly into 1 x 106 cells, incubate, and analyze.

As a cell-impermeant dye, SYTOX Green enters the cell upon loss of membrane integrity and binds to DNA, thereby acting as a counterstain that can be analyzed when excited at 488 nm and the emission captured at a peak of 523 nm. SYTOX Green reagent can be used in both mammalian and bacterial cells and is often included in multiplex assays.

사양
세포 투과성Impermeant
설명SYTOX™ Green Ready Flow™ Reagent
검출 방법Fluorescence
염료 유형SYTOX™ Green Ready Flow™
형태Solution
형식Bottle(s)
수량120 Assays
용해도DMSO (Dimethylsulfoxide)
색상Green
Emission523
Excitation Wavelength Range504 nm
용도(애플리케이션)Viability Assay
용도 (장비)Flow Cytometer
제품라인Ready Flow, Sytox
제품 유형Reagent
Unit SizeEach
구성 및 보관
Includes 2 dropper bottles, store at room temperature.
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자주 묻는 질문(FAQ)

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I would like to label only 100 µL of sample of the same cell density using a Ready Flow product. The manual notes 2 drops per 1 mL (1 X 10E6 cells/mL). May I scale down for smaller volumes?

Yes, you may scale up or down as needed, but we recommend keeping the cell density the same. There are 41 µL per drop, so 2 drops is 82 µL (for the 1 mL of sample). You need to only scale down for this 100 µL volume, which would be 8.2 µL.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the concentration of dye/reagent in the Ready Flow products?

The amount of dye or reagent in the Ready Flow products is proprietary. If you need to consider the exact amount of dye or reagent for your experiment, each of the Ready Flow reagents is available as a standalone product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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Lot 번호Certificate TypeDateCatalog Number(s)
3182983Certificate of Analysis2025년 5월 04일R37168
3143968Certificate of Analysis2025년 3월 17일R37168
2980694Certificate of Analysis2024년 9월 24일R37168
2800329Certificate of Analysis2024년 3월 14일R37168
2714535Certificate of Analysis2023년 9월 13일R37168
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