PCR tubes contribute to the reproducibility and accuracy of PCR experiments. Well-designed and well-engineered PCR tubes enable efficient heat transfer, provide sample protection, impart leak-proof sealing, and are compatible with a wide variety of thermal cyclers.
PCR tubes and strip tube formats
Tubes are an excellent choice for PCR/qPCR experiments from low to medium throughputs. Individual and strip tubes are two of the most common formats of PCR tubes.
Individual PCR tubes and PCR strip tubes
Individual PCR tubes are single tubes with an attached cap (Figure 1A). The individual PCR tubes are suitable for setting up the exact number of reactions as needed. The individual PCR tubes options are listed in Table 1.
PCR tube strips are commonly available as strips of 8 or 12 with attached or detached caps (Figure 1B). PCR tube strips are designed to work with both single-channel and multi-channel pipettes, which make it suitable for low- and medium-throughput experiments. Those with attached caps can be opened and closed independently. PCR tubes without attached caps can be sealed with domed, flat, or optically clear strip caps. The tube strips and cap strips are available as 8 or 12 strips. The strip tubes and strip caps are available separately or packaged together. Table 2 and Table 3 list the PCR strip tubes.
Figure 1. Various formats PCR tubes, strip tubes, and strip tube caps.
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The Thermo Scientific EasyStrip Plus Tube Strips come with individually attached caps. The attached caps are effective in preventing well-to-well sample contamination. Each tube is identified by an alphabet lettered on to the cap hinge which allows for easy identification. These are available with flat caps for PCR and optically clear for qPCR.
Cap strips for PCR tubes and PCR plates
Cap strips are used to seal multiple tubes simultaneously. Each strip is a set of individual caps attached together and can be used to seal PCR tube strips and PCR plates. Cap strips are convenient sealing solutions to help prevent sample loss and contamination. The caps are generally available in strips of 8 or 12 and may be domed, flat, mats, or ultra clear. These cap strips are compatible with Thermo Scientific PCR strip tubes and plates. The commonly used cap strips are found in Table 4.