Antibody Purification Using Protein G | Thermo Fisher Scientific

Antibody purification involves isolation of antibody from serum (polyclonal antibody), ascites fluid, or from the culture supernatant of a hybridoma cell line (monoclonal antibody). Protein G is a recombinant protein expressed in Escherichia coli (21.6 kDa; 2 IgG binding sites) and is recommended for purifying IgG from mouse, human, cow, goat, or sheep serum. The POROS MabCapture G Select Resin is a product that can be scaled up for bioprocess applications.

We offer a wide selection of base supports and formats to purify antibodies using the Protein G ligand. Our portfolio is designed to meet small-scale (screening) up to large-scale (bioprocess) needs.

Multiple formats—loose resin, spin columns and kits, FPLC cartridges, and 96-well filter plates enable antibody purification from microgram to kilogram scales
High performance—resins are designed to maximize protein yield and reduce background
Economical—pricing is similar to or better than our leading competitors

Choose the right Protein G product for antibody purification

Product links in the last row of each column

	Pierce Protein G Agarose	Pierce Protein G Plus Agarose	POROS MabCapture G Select
Bead or resin size	45–165 μm	45–165 μm	50 μm
Binding capacity	11–15 mg human IgG/mL resin	≥20 mg human IgG/mL resin	≥13 mg/mL human IgG/mL resin
Maximum linear flow rate	700 cm/hr	700 cm/hr	3000 cm/hr
Support	Crosslinked 6% beaded agarose	Crosslinked 6% beaded agarose	Polystyrene-divinylbenzene
Recommended application scale	batch, pilot	batch, pilot	batch, pilot, process
Formats available	loose beads, pre-packed spin columns and kits, chromatography cartridges, spin plates	loose beads	loose beads
Order product(s)	20398 (loose resin) 89926 (1 mL cartridge) 89927 (5 mL cartridge) 45204 (spin plates)	22851 (loose resin)	82082

Protein G spin columns, spin kits, and ready-to-use purification buffers

Catalog #	Name	Size	Price (USD)
89953	NAb™ Protein G Spin Columns, 0.2 mL	Each	415.00
89957	NAb™ Protein G Spin Columns, 1 mL	Each	854.00
89961	NAb™ Protein G Spin Columns, 5 mL	Each	782.00
89949	NAb™ Protein G Spin Kit, 0.2 mL	Each	462.00
89979	NAb™ Protein G Spin Kit, 1 mL	Each	374.00
21193	Pierce™ Recombinant Protein G, 5 mg	Each	235.65 Online Exclusive ~~274.00~~ Save 38.35 (14%)
21019	Pierce™ Protein G IgG Binding Buffer, 1 L	Each	108.65 Online Exclusive ~~124.00~~ Save 15.35 (12%)
21004	Pierce™ IgG Elution Buffer, 1 L	Each	112.65 Online Exclusive ~~129.00~~ Save 16.35 (13%)
21020	Pierce™ Gentle Ag/Ab Binding Buffer, pH 8.0, 1 L	Each	184.00
21027	Pierce™ Gentle Ag/Ab Elution Buffer, pH 6.6, 500 mL	Each	154.65 Online Exclusive ~~175.00~~ Save 20.35 (12%)
21030	Pierce™ Gentle Ag/Ab Binding and Elution Buffer Kit	Each	178.00

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Antibodies and Immunoassays Support Center

For Research Use Only. Not for use in diagnostic procedures.