Pierce™ Gentle Ag/Ab Elution Buffer, pH 6.6

Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such asRead more

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Catalog Number	Quantity
21027	500 mL
21013	3.75 L

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Catalog number 21027

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154.65

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175.00

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Quantity:

500 mL

Price (USD)

154.65

Online Exclusive

175.00

Save 20.35 (12%)

Each

Add to cart

Thermo Scientific Pierce IgG Binding and Elution Buffers ensure high-yield, nondenaturing antibody purification with IgG affinity purification supports, such as Protein A and Protein G agarose beads. The Gentle Ag/Ab Binding and Elution Buffer system uses near-neutral, high-salt conditions for elution, enabling even sensitive and labile antibodies (or other proteins) to be eluted from an affinity system without denaturation and inactivation. The phosphate-free binding buffer in this system is a necessary substitute for the Protein A binding buffer (pH 8), which precipitates with the Gentle Ag/Ab Elution Buffer.

Shared features of all Thermo Scientific Pierce IgG Binding and Elution Buffers:

• Ready to use—carefully formulated and prefiltered 1X solutions
• Three binding buffers—optimized for Protein A, Protein G or Protein A/G
• Two elution buffer options—acidic (pH 2.8) or high salt (near-neutral)
• Versatile—elution buffers are appropriate for antibody-antigen affinity purification

Products available for each antibody purification method:
• Protein A IgG Binding Buffer: pH 8.0; phosphate-based; contains glycerol and EDTA
• Protein A/G IgG Binding Buffer: pH 8.0; phosphate-based; contains EDTA
• Protein G IgG Binding Buffer: pH 5.0; contains 0.02% sodium azide
• IgG Elution Buffer: pH 2.8; amine-based
• Gentle Ag/Ab Binding Buffer: pH 8.0; phosphate-free; contains 0.05% sodium azide
• Gentle Ag/Ab Elution Buffer: pH 6.6; high salt (ionic strength)

Related Products
Pierce™ IgG Elution Buffer
Pierce™ Gentle Ag/Ab Binding Buffer, pH 8.0
Pierce™ Gentle Ag/Ab Binding and Elution Buffer Kit

For Research Use Only. Not for use in diagnostic procedures.

Specifications

FormLiquid

Quantity500 mL

Reagent TypeElution Buffer

Product LinePierce™

Product TypeElution Buffer

pH6.6

Unit SizeEach

Contents & Storage

4°C

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Frequently asked questions (FAQs)

After immobilizing my antibody, the first affinity purification of my protein worked well, but the protein failed to bind to the antibody during the second round of purification. What did I do wrong?

Low pH elution is the most commonly used method of elution for affinity purification, however, it could lead to denaturation of some antibodies which will negatively affect subsequent antigen binding. To prevent this from happening, after protein purification and column regeneration, immediately wash the column with neutralization or binding buffer and store at 4 degrees C in buffer containing an antimicrobial agent such as sodium azide.

Alternatively, use a near-neutral, high salt elution buffer such as Pierce Gentle Ag/Ab Elution Buffer (Cat. No. 21013, 21027, or 21030). For alternative elution buffers, please refer to this Tech Tip (https://tools.thermofisher.com/content/sfs/brochures/TR0027-Elution-conditions.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How many purifications can be performed using the same affinity column?

This varies with the stability of the immobilized protein and the type of elution buffer used. Columns can typically be reused at least 10 times without significant loss of activity. If the immobilized molecule is sensitive to low pH, consider using our Pierce Gentle Ag/Ab Elution Buffer (Cat. No. 21013, 21027 or 21030). For alternative elution buffers, please refer to this Tech Tip(http://tools.thermofisher.com/content/sfs/brochures/TR0027-Elution-conditions.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.