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Sequence specific proteases are utilized to cleave proteins into smaller fragments, or peptides. Trypsin is the protease of choice for protein digestion. However, separate or sequential digestion with alternative proteases, such as Glu-C, LysN, Lys-C, Asp-N or Chymotrypsin, can improve individual protein sequence coverage or generate unique peptide sequences for different MS applications. The Thermo Scientific Pierce Proteases are highly purified and modified for optimal protein digestion and validated for use in MS. The Thermo Scientific EasyPep Mini MS Sample Prep Kit provides pre-formulated reagents and a robust method for the preparation of high quality samples that are ready for MS analysis in less than 3 hours.
Use this rapid mass spec sample prep kit for protein extraction, digestion and peptide clean-up in 2-4 hours.
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Pierce Trypsin Protease, MS Grade | Pierce LysN Protease, MS Grade | Pierce Trypsin/Lys-C Protease Mix, MS-Grade | LysC Protease, MS Grade | |
Source | Porcine pancreatic extracts | Grifola frondosa | Porcine pancreatic extracts, Lysobacter enzymogenes | Lysobacter enzymogenes |
Cleavage specificity | Carboxyl side of arginine and lysine residues | Amino side of lysine residues | Carboxyl side of arginine and lysine residues | Carboxyl side of lysine residues |
Modified? | Yes, TPCK-treated and methylated | No | Yes (trypsin); No (LysC) | No |
Formats | Lyophilized or Frozen | Lyophilized | Lyophilized | Lyophilized |
Standard packaging | 20 or 100 µg/vial | 20 µg/vial | 20 or 100 µg/vial | 20 or 100 µg/vial |
Order now | Order now | Order now | Order now |
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Chymotrypsin | Glu-C Protease, MS Grade | Asp-N Protease, MS Grade | |
Source | Bovine pancreatic extracts | Staphylococcus aureus | Pseudomonas fragi |
Cleavage specificity | Carboxyl side of tyrosine, phenylalanine, tryptophan and leucine | Carboxyl side of glutamate | Amino side of aspartate residues |
Modified? | Yes, TLCK-treated | No | No |
Formats | Lyophilized | Lyophilized | Lyophilized |
Standard packaging | 4 x 25 µg/vial | 10 µg/vial | 2 µg/vial |
Order now | Order now | Order now |
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Pierce Trypsin Protease, MS Grade | Pierce LysN Protease, MS Grade | Pierce Trypsin/Lys-C Protease Mix, MS-Grade | LysC Protease, MS Grade | |
Source | Porcine pancreatic extracts | Grifola frondosa | Porcine pancreatic extracts, Lysobacter enzymogenes | Lysobacter enzymogenes |
Cleavage specificity | Carboxyl side of arginine and lysine residues | Amino side of lysine residues | Carboxyl side of arginine and lysine residues | Carboxyl side of lysine residues |
Modified? | Yes, TPCK-treated and methylated | No | Yes (trypsin); No (LysC) | No |
Formats | Lyophilized or Frozen | Lyophilized | Lyophilized | Lyophilized |
Standard packaging | 20 or 100 µg/vial | 20 µg/vial | 20 or 100 µg/vial | 20 or 100 µg/vial |
Order now | Order now | Order now | Order now |
![]() | ![]() | ![]() | |
---|---|---|---|
Chymotrypsin | Glu-C Protease, MS Grade | Asp-N Protease, MS Grade | |
Source | Bovine pancreatic extracts | Staphylococcus aureus | Pseudomonas fragi |
Cleavage specificity | Carboxyl side of tyrosine, phenylalanine, tryptophan and leucine | Carboxyl side of glutamate | Amino side of aspartate residues |
Modified? | Yes, TLCK-treated | No | No |
Formats | Lyophilized | Lyophilized | Lyophilized |
Standard packaging | 4 x 25 µg/vial | 10 µg/vial | 2 µg/vial |
Order now | Order now | Order now |
Quality of peptide samples achieved using Pierce Trypsin/Lys-C mix is as good as or better than protein samples prepared using another supplier’s trypsin/Lys-C mix.
The decision to perform in-solution or in-gel digestion is generally determined by the sample amount and/or its complexity.
In-solution digestion is recommended for small sample volumes with low to moderate complexity and is more amenable to high-throughput sample.
For highly complex samples, in-gel digestion has an inherent advantage because the SDS-PAGE workflow combines protein denaturation with separation, and provides a visual indication of the relative protein abundance in the sample, although peptide recovery is reduced.
We offer complete kits for both in-gel and in-solution sample processing.
Protein | LysN | Trypsin | Merged |
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Moesin | 21% | 24% | 37% |
Src substrate cortactin | 25% | 10% | 30% |
Glutamate dehydrogenase 1 | 16% | 12% | 28% |
Epoxide hydrolase 1 | 13% | 14% | 23% |
Percent sequence coverage of selected proteins in an A549 cell lysate upon in-solution digestion with LysN protease or trypsin. The total (merged) sequence coverage represents the combined coverage of separate LysN and trypsin digestions followed by LC-MS/MS analysis with CID.
For Research Use Only. Not for use in diagnostic procedures.