Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Ligases catalyze the joining of nucleic acid molecules enzymatically in an ATP-dependent manner. T4 DNA and RNA ligases are members of a large family of ligases and are derived from the T4 bacteriophage representing the most commonly used ligases in cloning protocols.
| T4 DNA Ligase | T4 RNA Ligase | |
|---|---|---|
| Applications |
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| Substrate |
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| Reaction catalyzed | ATP-dependent intra- and intermolecular formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. | ATP-dependent intra- and intermolecular formation of phosphodiester bonds between 5'-phosphate and 3'-hydroxyl termini of poly- and oligonucleotides, ssRNA and ssDNA. |
ss – single-stranded
ds – double-stranded
*Although ATP is routinely used as a phosphate donor, CTP, UTP, GTP, dATP, dTTP are equally effective.