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Gibco™
DMEM, low glucose, pyruvate, no glutamine, no phenol red
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells.Read more
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| Catalog Number | Quantity |
|---|---|
| 11054020 | 500 mL |
| 11880036 also known as 11880-036 | 10 x 500 mL |
| 11054001 | 1 L |
Catalog number 11054020
Price (USD)
50.65
Online Exclusive
54.50Save 3.85 (7%)
Each
In stock
Quantity:
500 mL
Price (USD)
50.65
Online Exclusive
54.50Save 3.85 (7%)
Each
Dulbecco's Modified Eagle Medium (DMEM) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM is modified as follows:
With: Low Glucose, Sodium Pyruvate
Without: L-glutamine, Phenol Red, HEPES
The complete formulation is available.
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate.
Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineHeLa, 293, Cos-7, and PC-12
Cell TypePrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesLow Glucose, Sodium Pyruvate
Without AdditivesNo Glutamine, No HEPES, No Phenol Red
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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What is the manganese concentration in DMEM? Do you offer manganese-free DMEM?
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Lot #Certificate TypeDateCatalog Number(s)
3141780Certificate of AnalysisJun 06, 202511054020
3199034Certificate of AnalysisJun 05, 202511054001
3150529Certificate of AnalysisMay 02, 202511054001, 11054020
3079797Certificate of AnalysisApr 25, 202511054001
3126875Certificate of AnalysisMar 09, 202511054001, 11054020
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
Manganese is not present in the formulation of our catalog DMEM media products.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.