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Gibco™
293fectin™ Transfection Reagent
293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized forRead more
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| Catalog Number | Quantity |
|---|---|
| 12347019 | 1 mL |
| 12347500 also known as 12347-500 | 15 mL |
| 12347750 also known as 12347-750 | 10 x 15 mL |
Catalog number 12347019
Price (USD)
676.65
Online Exclusive
734.00Save 57.35 (8%)
Each
Quantity:
1 mL
Price (USD)
676.65
Online Exclusive
734.00Save 57.35 (8%)
Each
293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized for transfecting suspension 293 human embryonic kidney cells (e.g., FreeStyle™ 293-F cells, Cat. No. R790-07) in defined, serum-free FreeStyle™ 293 Expression Medium (12338-018), and is intended for use with the FreeStyle™ 293 Expression System (K9000-01).
293fectin™ Transfection Reagent provides the following advantages:
- Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells
- Suspension FreeStyle™ 293-F cells may be transfected in FreeStyle™ 293 Expression Medium; no medium change is required
- Add 293fectin™ reagent complexes directly to cells in culture medium
- No need to remove complexes or change or add medium following transfection
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Transfection
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Product Line293fectin™
Product TypeTransfection Reagent
Quantity1 mL
Serum CompatibleYes
Cell TypeEstablished Cell Lines
Sample TypePlasmid DNA
Transfection TechniqueLipid-based Transfection
Unit SizeEach
Contents & Storage
Each unit contains one vial of reagent. Store at 4°C.
293fectin™ is guaranteed stable for 6 months when stored properly.
293fectin™ is guaranteed stable for 6 months when stored properly.
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Lot #Certificate TypeDateCatalog Number(s)
3162145Certificate of AnalysisMar 24, 202512347019
3056377Certificate of AnalysisMar 12, 202512347500
3050080Certificate of AnalysisFeb 19, 202512347019
2988415Certificate of AnalysisOct 08, 202412347019
2839677Certificate of AnalysisJul 01, 202412347019
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Safety Data Sheets
SDSScientific Resources
Product Information
Frequently asked questions (FAQs)
Both FreeStyle Max Reagent and 293fectin Transfection Reagent provide similar levels of high transfection efficiency; however, FreeStyle Max Reagent has lower cytotoxicity and hence results in higher protein yields. Additionally, FreeStyle Max Reagent is animal-origin free.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Yes, all of our lipid transfection reagents are stable at room temperature for months.
Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.
In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.
For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.
Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.
Citations & References (3)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Molecular dissection of the alpha-dystroglycan- and integrin-binding sites within the globular domain of human laminin-10.
Journal:J Biol Chem
PubMed ID:14701821
'The adhesive interactions of cells with laminins are mediated by integrins and non-integrin-type receptors such as alpha-dystroglycan and syndecans. Laminins bind to these receptors at the C-terminal globular domain of their alpha chains, but the regions recognized by these receptors have not been mapped precisely. In this study, we sought
Binding of anthrax toxin to its receptor is similar to alpha integrin-ligand interactions.
Journal:J Biol Chem
PubMed ID:14507921
The secreted protein toxin produced by Bacillus anthracis contributes to virulence of this pathogen and can cause many of the symptoms seen during an anthrax infection, including shock and sudden death. The cell-binding component of anthrax toxin, protective antigen, mediates entry of the toxin into cells by first binding directly
T cell immunoglobulin mucin-3 crystal structure reveals a galectin-9-independent ligand-binding surface.
Journal:Immunity
PubMed ID:17363302
The T cell immunoglobulin mucin (Tim) family of receptors regulates effector CD4(+) T cell functions and is implicated in autoimmune and allergic diseases. Tim-3 induces immunological tolerance, and engagement of the Tim-3 immunoglobulin variable (IgV) domain by galectin-9 is important for appropriate termination of T helper 1-immune responses. The 2
3 total citations