DiD' oil; DiIC18(5) oil (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine Perchlorate)
DiD' oil; DiIC<sub>18</sub>(5) oil (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine Perchlorate)
Invitrogen™

DiD' oil; DiIC18(5) oil (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindodicarbocyanine Perchlorate)

The far-red fluorescent, lipophilic carbocyanine DiD is a longer-wavelength DiI analog. It is an oil at room temperature and weaklyRead more
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Catalog NumberQuantity
D30725 mg
Catalog number D307
Price (KRW)
384,000
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Ends: 30-Jun-2025
426,000
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Price (KRW)
384,000
Online offer
Ends: 30-Jun-2025
426,000
Save 42,000 (10%)
Each
Add to cart
Ask our AI about this Product
The far-red fluorescent, lipophilic carbocyanine DiD is a longer-wavelength DiI analog. It is an oil at room temperature and weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (∼1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorRed
Detection MethodFluorescence
Emission665 nm
Excitation Wavelength Range644 nm
For Use With (Equipment)Fluorescence Microscope
Molecular Weight (g/mol)959.92
Quantity25 mg
Shipping ConditionRoom Temperature
Product TypeLiphophilic Tracer
SubCellular LocalizationCell Membranes, Lipids
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.
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Figures

Fluorescence spectra

Fluorescence spectra

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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3101959Certificate of AnalysisDec 07, 2024D307
2806861Certificate of AnalysisDec 19, 2023D307
2451275Certificate of AnalysisAug 29, 2022D307
2381692Certificate of AnalysisAug 08, 2021D307
2298149Certificate of AnalysisJan 20, 2021D307
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Safety Data Sheets

Frequently asked questions (FAQs)

This is expected. DiD (which is far-red fluorescent) is never as uniform as DiI (which is orange fluorescent). If uniformity is desired, try increasing the label time and concentration, but it still isn't likely to be as uniform as DiI. CellMask Deep Red Plasma Membrane stain is much more uniform and is about the same wavelength as DiD. However, if you intend to do cell tracking over days, CellMask stain has not been tried for that application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Select the dye that is compatible with your available excitation source(s) and emission filter set/channels. The solid, paste and crystal forms can be applied directly to neurons in tissues. For labeling cells in culture or microinjection, the lipophilic dyes in solution or solid form can be used.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Lipophilic cyanine dyes are preferred for this sort of assay, since they insert into cellular membranes and then, upon fusion, are shared by the fused cells as the membranes are shared. For example, one cell population can be labeled with DiI (orange-red) and another cell population can be labeled with DiO (green), and when the cells fuse, the combined color appears yellow (when imaged with a dual-bandpass filter set).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (69)

Citations & References
Abstract
Simultaneous measurement of RBC velocity, flux, hematocrit and shear rate in vascular networks.
Authors:Kamoun WS, Chae SS, Lacorre DA, Tyrrell JA, Mitre M, Gillissen MA, Fukumura D, Jain RK, Munn LL,
Journal:Nat Methods
PubMed ID:20581828
Not all tumor vessels are equal. Tumor-associated vasculature includes immature vessels, regressing vessels, transport vessels undergoing arteriogenesis and peritumor vessels influenced by tumor growth factors. Current techniques for analyzing tumor blood flow do not discriminate between vessel subtypes and only measure average changes from a population of dissimilar vessels. We ... More
Dynamics of a chemoattractant receptor in living neutrophils during chemotaxis.
Authors:Servant G, Weiner OD, Neptune ER, Sedat JW, Bourne HR
Journal:Mol Biol Cell
PubMed ID:10198064
'Persistent directional movement of neutrophils in shallow chemotactic gradients raises the possibility that cells can increase their sensitivity to the chemotactic signal at the front, relative to the back. Redistribution of chemoattractant receptors to the anterior pole of a polarized neutrophil could impose asymmetric sensitivity by increasing the relative strength ... More
Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
Authors:Brieher WM, Gumbiner BM
Journal:J Cell Biol
PubMed ID:8034750
'Treatment of Xenopus animal pole tissue with activin results in the induction of mesodermal cell types and a dramatic elongation of the tissue. The morphogenetic movements involved in the elongation appear similar to those in normal gastrulation, which is driven by cell rearrangement and cell intercalations. We have used this ... More
Chemical imaging of tissue in vivo with video-rate coherent anti-Stokes Raman scattering microscopy.
Authors:Evans CL, Potma EO, Puoris'haag M, Côté D, Lin CP, Xie XS
Journal:Proc Natl Acad Sci U S A
PubMed ID:16263923
'Imaging living organisms with molecular selectivity typically requires the introduction of specific labels. Many applications in biology and medicine, however, would significantly benefit from a noninvasive imaging technique that circumvents such exogenous probes. In vivo microscopy based on vibrational spectroscopic contrast offers a unique approach for visualizing tissue architecture with ... More
In vivo migration of dendritic cells differentiated in vitro: a chimpanzee model.
Authors:Barratt-Boyes SM, Watkins SC, Finn OJ
Journal:J Immunol
PubMed ID:9144465
'Dendritic cells with potent Ag-presenting function can be propagated from peripheral blood using recombinant cytokines, and these cells have potential usefulness as immunotherapeutic agents in the treatment of cancer and other disease states. However, it is not known if these in vitro differentiated dendritic cells have the capacity to migrate ... More
69 total citations

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