Advanced RPMI 1640 Medium

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Gibco™

Advanced RPMI 1640 Medium

Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cellsRead more

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Catalog Number	Quantity
12633012	500 mL
12633020	10 x 500 mL

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Catalog number 12633012

Price (USD)

55.50

Each

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Quantity:

500 mL

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Price (USD)

55.50

Each

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Advanced RPMI (Roswell Park Memorial Institute) 1640 is a widely used basal medium that allows the culture of mammalian cells with reduced Fetal Bovine Serum (FBS) supplementation. Compared to classic RPMI 1640, serum supplementation can be reduced by 50-90% with no change in growth rate or morphology.ⁱ Cells successfully cultured in Advanced RPMI 1640, with no adaptation, include Sp2, Vero, Raji, Daudi, and Jurkat.

This Advanced RPMI 1640 is manufactured as follows:

With: Glucose, Non Essential Amino Acids, Sodium pyruvate, Phenol Red

Without: L-glutamine, HEPES

The complete formulation is available.

Gibco™ Advanced RPMI 1640 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Product Use

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Advanced RPMI 1640 requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement. Many cell lines do not require adaptation to this media. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced RPMI 1640 uses a sodium bicarbonate buffer system (3.7 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

ⁱAdvanced Media: Reduced Sera Solutions. Quest, 2004. 1.3:26-30.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell LineSp2, Vero, Raji, Daudi, and Jurkat

Concentration1 X

Manufacturing QualitycGMP-compliant under the ISO 13485 standard

Product LineGibco™

Product TypeRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)

Quantity500 mL

Shelf Life12 Months From Date of Manufacture

Shipping ConditionRoom Temperature

ClassificationAnimal Origin

FormLiquid

Serum LevelReduced Serum

SterilitySterile-filtered

Sterilization MethodSterile-filtered

With AdditivesGlucose, Phenol Red, Sodium Pyruvate, Non Essential Amino Acids (NEAA)

Without AdditivesNo Glutamine, No HEPES

Unit SizeEach

Contents & Storage

Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture

Media Formulations

12633 - Advanced RPMI 1640

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Frequently asked questions (FAQs)

What is the glucose concentration in Advanced RPMI 1640 Medium (Cat. No. 12633012, 12633020)?

Advanced RPMI 1640 Medium (Cat. No. 12633012, 12633020) contains glucose (D-Glucose or Dextrose) at a concentration of 2 g/L.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer Advanced RPMI 1640 Medium (Cat. No. 12633012 or 12633020) without phenol red?

We do not offer Advanced RPMI 1640 medium without phenol red as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Advanced RPMI 1640 Medium and other RPMI 1640 formulations?

Advanced Media are widely used basal media, which require 50-90% less Fetal Bovine Serum (FBS) supplementation compared to conventional basal media formulations. The Advanced media is unique due to the addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

View all Advanced RPMI 1640 Medium FAQs