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Gibco™
RPMI 1640 Medium, HEPES
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park MemorialRead more
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| Catalog Number | Quantity |
|---|---|
| 22400089 | 500 mL |
| 22400097 | 100 mL |
| 22400105 | 10 x 500 mL |
| 22400071 | 1000 mL |
| 22400121 | 6 x 1000 mL |
Catalog number 22400089
Price (USD)
50.65
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54.25Save 3.60 (7%)
Each
In stock
Quantity:
500 mL
Price (USD)
50.65
Online Exclusive
54.25Save 3.60 (7%)
Each
RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This RPMI is modified as follows:
With: L-glutamine, HEPES, Phenol Red
The complete formulation is available.
Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas
Cell TypeLeukemic Cells
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, HEPES, Phenol Red
Without AdditivesNo Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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Lot #Certificate TypeDateCatalog Number(s)
3126871Certificate of AnalysisJun 22, 202522400097, 22400089, 22400071
3166067Certificate of AnalysisJun 18, 202522400097, 22400089, 22400071
3140680Certificate of AnalysisJun 11, 202522400097, 22400089, 22400071
3141605Certificate of AnalysisMay 31, 202522400097, 22400089, 22400071
3140683Certificate of AnalysisMay 31, 202522400097, 22400089, 22400071
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Citations & References (12)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Association of Bcr-Abl with the Proto-oncogene Vav Is Implicated in Activation of the Rac-1 Pathway.
Journal:J Biol Chem
PubMed ID:11790798
'Vav is a guanine nucleotide exchange factor for the Rho/Rac family predominantly expressed in hematopoietic cells and implicated in cell proliferation and cytoskeletal organization. The oncogenic tyrosine kinase Bcr-Abl has been shown to activate Rac-1, which is important for Bcr-Abl induced leukemogenesis. Previous studies by Matsuguchi et al. (Matsuguchi, T.,
A functional role for the B56 alpha-subunit of protein phosphatase 2A in ceramide-mediated regulation of Bcl2 phosphorylation status and function.
Journal:J Biol Chem
PubMed ID:11929874
'Recently it has been shown that the potent apoptotic agent ceramide activates a mitochondrial protein phosphatase 2A (PP2A) and promotes dephosphorylation of the anti-apoptotic molecule Bcl2 (Ruvolo, P. P., Deng, X., Ito, T., Carr, B. K., and May, W. S. (1999) J. Biol. Chem. 274, 20296-20300). In cells expressing Bcl2,
Cell-penetrating Peptide enhanced intracellular Raman imaging and photodynamic therapy.
Journal:
PubMed ID:23659475
'We present the application of a theranostic system combining Raman imaging and the photodynamic therapy (PDT) effect. The theranostic nanoplatform was created by loading the photosensitizer, protoporphyrin IX, onto a Raman-labeled gold nanostar. A cell-penetrating peptide, TAT, enhanced intracellular accumulation of the nanoparticles in order to improve their delivery and
The S18 ribosomal protein is a putative substrate for Ca2+/calmodulin-activated protein kinase II.
Journal:J Biol Chem
PubMed ID:12145273
'The delta-isoform of Ca(2+)/calmodulin-activated protein kinase II (CaMK II) is abundantly expressed in vascular smooth muscle, but relatively little is known about its regulation or its potential cellular substrates. There are few, if any, known substrates of CaMK II that are physiologically relevant in vascular smooth muscle cells. Studies presented
The expression of Sprouty1, an inhibitor of fibroblast growth factor signal transduction, is decreased in human prostate cancer.
Journal:Cancer Res
PubMed ID:15256439
A considerable body of evidence indicates that alterations of fibroblast growth factors (FGFs) and their receptors contribute to prostate cancer progression. Recently, a new family of regulators of FGF activity has been identified. The Sprouty gene family negatively regulates FGF signaling in a variety of systems and could potentially limit
12 total citations