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Gibco™
Cell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt Solution
Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Hank's Balanced Salt Solution (HBSS).
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| Catalog Number | Quantity |
|---|---|
| 13150016 | 100 mL |
Catalog number 13150016
Price (USD)
53.00
Each
In stock
Quantity:
100 mL
Price (USD)
53.00
Each
Gibco Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Hanks' Balanced Salt Solution (HBSS). It is suitable for the gentle dissociation of mammalian cells for studies such as ligand binding, flow cytometry, and immunohistochemistry, that require intact cell surface proteins. Cell Dissociation Buffer works well with lightly adherent cell lines, such as HeLa and NIH 3T3, but is not recommended for the routine passaging of strongly adherent cells.
Gibco Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Hank's Balanced Salt Solution (HBSS). Gibco Cell Dissociation Buffer is suitable for gentle dissociation of mammalian cells for studies requiring intact cell surface proteins such as ligand binding, flow cytometry, and immunohistochemistry. Gibco Cell Dissociation Buffer works well with lightly adherent cell lines such as HeLa and NIH 3T3, but is not recommended for routine passage of strongly adherent cells.
Cell Culture, Mammalian Cell Culture
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell LineHeLa, NIH 3T3
ChelatorsEDTA
Quantity100 mL
RemarksRoom Temperature Stable
Shelf Life24 Months
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
Product TypeCell Culture Dissociation Reagent
SterilitySterile-filtered
With AdditivesEDTA, Earle's Balanced Salt Solution (EBSS)
Unit SizeEach
Contents & Storage
Storage conditions: 15–30°C
Shipping conditions: ambient
Shelf life: 24 months from date of manufacture
Shipping conditions: ambient
Shelf life: 24 months from date of manufacture
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Lot #Certificate TypeDateCatalog Number(s)
3146841Certificate of AnalysisJun 06, 202513150016
2572398Certificate of AnalysisJan 29, 202513150016
2572396Certificate of AnalysisJan 25, 202513150016
A0466141Certificate of AnalysisSep 13, 2024131500250, 131500100, 131501000, 13150016
A0462239Certificate of AnalysisApr 29, 2024131500250, 131500100, 131501000, 13150016
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SDSCitations & References (1)
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Citations & References
Abstract
The Low Density Lipoprotein Receptor-related Protein Mediates Fibronectin Catabolism and Inhibits Fibronectin Accumulation on Cell Surfaces.
Journal:J Biol Chem
PubMed ID:11867643
Low density lipoprotein receptor-related protein (LRP) is a member of the low density lipoprotein receptor family, which functions as an endocytic receptor for diverse ligands. In this study, we demonstrate that murine embryonic fibroblasts (MEF-2 cells) and 13-5-1 Chinese hamster ovary cells, which are LRP-deficient, accumulate greatly increased levels of
1 total citations