DPBS, no calcium, no magnesium

This product is manufactured in our Paisley, Scotland facility and is intended for sale in Europe, Africa, and the Middle East—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Grand Island, US, is recommended for our customers in North America, Latin America, and the Asia/Pacific region. Click to view.

Certificates

SDS

Citations & References (7)

Gibco™

DPBS, no calcium, no magnesium

Name: DPBS, no calcium, no magnesium
SKU: 14190094

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washingRead more

Change view

Catalog Number	Quantity	Packaging Type
14190094 also known as 14190-094	500 mL	Bottle
14190144	500 mL	Bottle
14190250	10 x 500 mL	Bottle
14190136	1000 mL	Bottle
14190235	6 x 1000 mL	Bottle
14190342	5 L	Bag
14190359	10 L	Bag
14190367	20 L	Bag

8 Options

Catalog number 14190094

also known as 14190-094

Price (USD)

고객 센터

Quantity:

500 mL

Packaging Type:

Bottle

Customize this product

Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation.

We offer a variety of Gibco™ DPBS formulations for a range of cell culture applications.

This DPBS is modified as follows:

Without: Calcium, Magnesium, Phenol Red

The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.

Specifications

AdditiveNo Sodium Pyruvate

ClassificationAnimal Origin-Free

ColorClear

Dilution1 X

Endotoxin Level≤ 0.50 EU/mL

For Use With (Application)Mammalian Cell Culture

Manufacturing QualitycGMP-compliant under the ISO 13485 standard

Osmolality270 - 300 mOsm/kg

Packaging TypeBottle

Phenol Red IndicatorNo Phenol Red

Product LineGibco™

Quantity500 mL

Shipping ConditionRoom Temperature

SterilitySterile-filtered

FormLiquid

Product TypeDPBS

Solution TypeDulbecco's Phosphate Buffered Saline

pH7.0 to 7.3

Unit SizeEach

Contents & Storage

Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 36 months from date of manufacture

Explore all Gibco Balanced Salt Solutions to enable increased culture stability ›

Media Formulations

14190 - DPBS, no calcium, no magnesium

This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Frequently asked questions (FAQs)

What is the shelf life of DPBS after it has been opened?

We cannot provide an official expiration date for open bottles as this depends on the customer's use. Once opened, Thermo Fisher Scientific can no longer guarantee the performance of any product. However, if the storage conditions are followed as recommended, the stated expiration date (36 months from the date of manufacture) should apply to opened bottles as DPBS is extremely stable.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of DPBS (Cat. No. 14190136)?

DPBS has a shelf life of 36 months from the date of manufacture, when stored as recommended (15-30 degrees C).

Does Diploid Growth Serum-Reduced Medium (SRM) contain L-glutamine?

The basal Diploid Growth Serum-Reduced Medium already contains 6 mM L-glutamine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do I have to adjust my infection parameters when using Diploid Production Serum-Free Medium (SFM)?

We recommend evaluating performance with Diploid Production Serum-Free Medium using current conditions, however, multiplicity of infection, time of infection, and time of harvest may be different than with conventional media. Closely monitor cells for cytopathic effect and evaluate viral titers at multiple time points.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Diploid Growth Serum-Reduced Medium (SRM) be supplemented with different types of serum?

Diploid Growth Serum-Reduced Medium can be supplemented with Fetal Bovine Serum, Newborn Calf Serum, Donor Bovine Serum with Iron, or Bovine Serum.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all DPBS, no calcium, no magnesium FAQs

해당 제품을 검색한 고객들이 함께 검색한 제품

PBS, pH 7.4, 1 x 500 mL, Bottle

Catalog number: 10010023

46,000 / Each

Penicillin-Streptomycin (10,000 U/mL), 100 mL

Catalog number: 15140122

49,000 / Each

DMEM, High Glucose, Pyruvate

Catalog number: 11995065

53,000 / Each

Trypsin-EDTA(0.25%), phenol red

Catalog number: 25200056

32,000 / Each

RPMI 1640 Medium

Catalog number: 11875093

53,000 / Each

UltraPure™ DNase/RNase-Free Distilled Water, 500 mL

Catalog number: 10977015

66,000 / Each

DMEM, high glucose

Catalog number: 11965092

51,000 / Each

Trypsin-EDTA (0.05%), phenol red, 100 mL

Catalog number: 25300054

32,000 / Each

DPBS, calcium, magnesium, 500 mL

Catalog number: 14040133

47,000 / Each

DMEM, high glucose, GlutaMAX™ Supplement, pyruvate, 500 mL

Catalog number: 10569010

67,000 / Each

TrypLE™ Express Enzyme (1X), no phenol red, 100 mL

Catalog number: 12604013

47,000 / Each

Fetal Bovine Serum, Value, bottle, 500 mL

Catalog number: A5256701

506,000 / Each

TrypLE™ Express Enzyme (1X), phenol red, 100 mL

Catalog number: 12605010

47,000 / Each

GlutaMAX™ Supplement, 100 mL

Catalog number: 35050061

81,000 / Each

Fetal Bovine Serum (FBS), Premium, bottle, 500 mL

Catalog number: A5670701

768,000 / Each

Documents & Downloads

Certificates

Search by lot number or partial lot number

Lot #Certificate TypeDateCatalog Number(s)

3144480Certificate of AnalysisJul 10, 202514190094, 14190359, 14190144, 14190342, 14190367, 14190136, 14190086, 14190240

3013565Certificate of AnalysisJul 05, 202514190094, 14190359, 14190144, 14190342, 14190367, 14190136, 14190086, 14190240

3166043Certificate of AnalysisJul 05, 202514190094, 14190359, 14190144, 14190342, 14190367, 14190136, 14190086, 14190240

3189212Certificate of AnalysisJul 03, 202514190094, 14190359, 14190144, 14190342, 14190367, 14190136, 14190086, 14190240

3168636Certificate of AnalysisJul 03, 202514190094, 14190359, 14190144, 14190342, 14190367, 14190136, 14190086, 14190240

5 results displayed, search above for a specific certificate

Request a Certificate

Safety Data Sheets

SDS

Scientific Resources

14190 - DPBS, no calcium, no magnesium

GIBCO - Bag Capabilities

Universal Bag Brochure - Its in the Bag

Application Note: Simple and accurate monitoring of adipogenesis

Flyer: Choose the right Balanced Salt Solution for your cell culture

Product Information

Product Information Sheet: Buffered Saline Solutions

Citations & References (7)

Search citations by name, author, journal title or abstract text

Citations & References

Abstract

A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.

Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T

Journal:Experimental cell research

PubMed ID:38499143

Effects of an indole derivative on cell proliferation, transfection, and alternative splicing in production of lentiviral vectors by transient co-transfection.

Authors:Mier NC,Roper DK

Journal:PloS one

PubMed ID:38833479

Lentiviral vectors derived from human immunodeficiency virus type I are widely used to deliver functional gene copies to mammalian cells for research and gene therapies. Post-transcriptional splicing of lentiviral vector transgene in transduced host and transfected producer cells presents barriers to widespread application of lentiviral vector-based therapies. The present study ... More

Method for Large-scale Production of hIPSC Spheroids.

Authors:Lemarié L,Courtial EJ,Sohier J

Journal:Bio-protocol

PubMed ID:38618177

Stem cell spheroids are rapidly becoming essential tools for a diverse array of applications ranging from tissue engineering to 3D cell models and fundamental biology. Given the increasing prominence of biotechnology, there is a pressing need to develop more accessible, efficient, and reproducible methods for producing these models. Various techniques ... More

Macrophage foam cell formation with native low density lipoprotein.

Authors: Kruth Howard S; Huang Wei; Ishii Itsuko; Zhang Wei-Yang;

Journal:J Biol Chem

PubMed ID:12118008

'This investigation has elucidated a mechanism for development of macrophage foam cells when macrophages are incubated with native low density lipoprotein (LDL). LDL is believed to be the main source of cholesterol that accumulates in monocyte-derived macrophages within atherosclerotic plaques, but native LDL has not previously been shown to cause ... More

Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns.

Authors:Sanchez-Freire V, Ebert AD, Kalisky T, Quake SR, Wu JC,

Journal:Nat Protoc

PubMed ID:22481529

'Single-cell quantitative real-time PCR (qRT-PCR) combined with high-throughput arrays allows the analysis of gene expression profiles at a molecular level in approximately 11 h after cell sample collection. We present here a high-content microfluidic real-time platform as a powerful tool for comparatively investigating the regulation of developmental processes in single ... More

7 total citations