Nunc™ Lab-Tek™ II Chambered Coverglass
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Nunc™ Lab-Tek™ II Chambered Coverglass
Thermo Scientific™

Nunc™ Lab-Tek™ II Chambered Coverglass

Culture and view living cells on a single device. This coverglass has optimum optical characteristics for high-magnification microscopy and confocal image analysis.
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Catalog NumberWellsMaterialDescriptionUnit Size
155360Promo Image1Borosilicate Glass (1.5)1-well Chambered Coverglass w/ non-removable wellCase of 96
155360PKPromo Image
also known as 155360
1Borosilicate Glass (1.5)1-well Chambered Coverglass w/ non-removable wellPack of 16
155379Promo Image2Borosilicate Glass (1.5)2-well Chambered Coverglass w/ non-removable wellsCase of 96
155379PKPromo Image
also known as 155379
2Borosilicate Glass (1.5)2-well Chambered Coverglass w/ non-removable wellsPack of 16
155382Promo Image4Borosilicate Glass (1.5)4-well Chambered Coverglass w/ non-removable wellsCase of 96
155382PKPromo Image4Borosilicate Glass (1.5)4-well Chambered Coverglass w/ non-removable wellsPack of 16
155409Promo Image8Borosilicate Glass (1.5)8-well Chambered Coverglass w/ non-removable wellsCase of 96
155409PKPromo Image8Borosilicate Glass (1.5)8-well Chambered Coverglass w/ non-removable wellsPack of 16
Catalog number 155360
Price (USD)
1,511.65
Online Exclusive
1,636.00
Save 124.35 (8%)
Case of 96
Estimated availability date  Pending  
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Wells:
1
Material:
Borosilicate Glass (1.5)
Description:
1-well Chambered Coverglass w/ non-removable well
Unit Size:
Case of 96
Recurring order eligible. Learn more »
Price (USD)
1,511.65
Online Exclusive
1,636.00
Save 124.35 (8%)
Case of 96
Add to cart
Ask our AI about this Product
Culture, examine, and analyze living cells on a single device. Thermo Scientific™ Nunc™ Lab-Tek™ II Chambered Coverglass with a No. 1.5 borosilicate glass bottom offers the ideal optical characteristics required for high-magnification microscopy and confocal image analysis. Available in 1-, 2-, 4-, and 8-well formats. The upper chamber structure cannot be removed from the coverglass.
Features:
  • Media chamber securely mounted to the No. 1.5 borosilicate coverglass by biocompatible acrylic adhesive
  • The polystyrene chamber is not removable and requires inverted microscope for viewing
  • Gas exchange achieved with minimal evaporation through tabbed polystyrene cover

Ordering Information:

  • Can be purchased in case of 96 or pack of 16.
  • Shipped sterile in convenient stackable incubation trays.


Warranty: 90 days

 

WARNING: Cancer – www.P65Warnings.ca.gov
Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.
Specifications
Closure ColorClear
Description1-well Chambered Coverglass w/ non-removable well
IncludesClear polystyrene lid with handles on the top and side for aseptic manipulations
Lid MaterialPolystyrene
No. per Pack16
Culture Area8.6 cm2
Dimensions (LxW)55 x 24 mm
MaterialBorosilicate Glass (1.5)
SterilitySterile
Surface TreatmentCell Culture
Thickness (Metric)0.16 to 0.19 mm
Volume (Metric) Working2.0 to 4.5 mL
Wells1
Unit SizeCase of 96
ECM
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Frequently asked questions (FAQs)

What can cause poor resolution when observing cells under a microscope?

Please review the following possible causes and solutions:
- The microscope could be dirty from fingerprints or oils. We recommend cleaning your microscope prior to use and always after oil immersion. When using oil immersion, it is important to use the specific brand of oil designed and recommended for that particular microscope.
- An incorrect type of coverslip or coverglass can cause poor resolution. We recommend Nunc Lab-Tek Chambered Coverglass (made from 1.0 borosilicate glass) and Nunc Lab-Tek II Chambered Coverglass (made from 1.5 borosilicate glass) for the best resolution. Plastic coverslips may affect resolution at high magnification due to refractive index differences between the glass and plastic.
- An incorrect thickness for the coverglass can cause poor resolution. Please consult your microscope's manual for the best information.
- The coverslipping procedure may affect resolution. A clear seal is required, free of debris and particles. When performing oil immersion, bubbles may result in poor resolution under high magnification.

How does cell culturing on Nunc Lab-Tek Chambered Coverglass compare with Nunc Lab-Tek II Chambered Coverglass?

Lab-Tek and Lab-Tek II chambered coverglass products can be plated with cells, incubated, and handled in the same way. The two products differ in the thickness of the glass and the chamber size. Lab-Tek coverglass uses Borosilicate coverglass No. 1 that is 0.13 - 0.16 mm thick and Lab-Tek II coverglass uses Borosilicate coverglass No. 1.5 thath is 0.16 - 0.19 mm thick. Lab-Tek II coverglass chambers hold 10 - 50% more media than Lab-Tek coverglass chambers, depending on the format. Studies have shown that Lab-Tek II chambered coverglass works well with fastidious cells such as neuronal, as well as poorly adherent cell lines.

What stains and fixatives are compatible with Lab-Tek chambered slides and coverglasses?

Lab-Tek products are compatible with all common fixatives, including paraformaldehyde. Acetone at 100% and some mixtures of acetone and alcohols are not compatible with the polystyrene upper well structure and will result in clouding or other effects of chemical attack. We encourage careful testing within planned operating conditions before working with high-value samples.

What factors must I consider when working with Lab-Tek and Lab-Tek II chambered coverglass products?

Lab-Tek chambered coverglasses are not accessories to Lab-Tek chamber slides, but another complete product for cell culture. The chambers are simply adhered to a borosilicate coverglass (to serve as the culture surface) rather than a soda lime glass or Permanox microscope slide. Most customers select this format for compatibility with confocal imaging systems that require a #1.0, approx. 120 micron (Lab-Tek) or #1.5, approx. 180 micron (Lab-Tek II) substrate. Because the coverglass is very thin and fragile, the chambered coverglasses (unlike chamber slides) cannot be disassembled.

What are the different options available in Lab-Tek II chamber slides?

Lab-Tek II chambered glass slide systems are available in our standard high-purity water washed culture treatment or our “CC2” surface modification. The latter is a proprietary passive treatment that resembles poly-D-lysine, often improving culture performance when working with fastidious cells such as neuronal cultures.

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