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Gibco™
McCoy's 5A (Modified) Medium
McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, establishedRead more
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| Catalog Number | Quantity |
|---|---|
| 16600108 | 10 x 500 mL |
| 16600082 | 500 mL |
Catalog number 16600108
Price (USD)
330.65
Online Exclusive
360.00Save 29.35 (8%)
Each
In stock
Quantity:
10 x 500 mL
Price (USD)
330.65
Online Exclusive
360.00Save 29.35 (8%)
Each
McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
This McCoy's 5A is modified as follows:
| With | Without |
| • High Glucose | • Sodium Pyruvate |
| • L-glutamine | • HEPES |
| • Bacto-peptone | |
| • Phenol Red |
The complete formulation is available.
Using McCoy's 5A (modified) Medium
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Specifications
Cell LineRat fibroblasts
Cell TypeBiopsy Tissues
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeMcCoy's 5A Modified Medium
Quantity10 x 500 mL
Shelf Life12 Months From Date of Manufacture
ClassificationAnimal Origin
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red, Bacto-peptone
Without AdditivesNo HEPES, No Sodium Pyruvate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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Photobleach protection using ProLong® Live Antifade Reagent
Photobleach protection using ProLong® Live Antifade Reagent
Photobleach protection using ProLong® Live Antifade Reagent
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Lot #Certificate TypeDateCatalog Number(s)
3140672Certificate of AnalysisJun 28, 202516600082
3224547Certificate of AnalysisJun 04, 202516600082
3188186Certificate of AnalysisMay 09, 202516600082
3115092Certificate of AnalysisApr 18, 202516600082
2645334Certificate of AnalysisJan 29, 202516600082
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.
Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.
Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Try changing the medium or serum. Compare media formulations for differences in glucose, amino acids, and other components. Compare an old lot of serum with a new lot. Increase initial cell inoculums. Lastly, adapt cells sequentially to new medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
This can occur if cells are overly trypsinized. Trypsinize for a shorter time or use less trypsin. Mycoplasma contamination could also cause this problem. Segregate your culture and test for mycoplasma infection. Lastly, check for attachment factors in the medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Citations & References (3)
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Citations & References
Abstract
Novel kelch-like protein, KLEIP, is involved in actin assembly at cell-cell contact sites of Madin-Darby canine kidney cells.
Journal:Mol Biol Cell
PubMed ID:14668487
Dynamic rearrangements of cell-cell adhesion underlie a diverse range of physiological processes, but their precise molecular mechanisms are still obscure. Thus, identification of novel players that are involved in cell-cell adhesion would be important. We isolated a human kelch-related protein, Kelch-like ECT2 interacting protein (KLEIP), which contains the broad-complex, tramtrack,
DMH1, a novel BMP small molecule inhibitor, increases cardiomyocyte progenitors and promotes cardiac differentiation in mouse embryonic stem cells.
Journal:PLoS One
PubMed ID:22848549
The possibility of using cell-based therapeutics to treat cardiac failure has generated significant interest since the initial introduction of stem cell-based technologies. However, the methods to quickly and robustly direct stem cell differentiation towards cardiac cell types have been limited by a reliance on recombinant growth factors to provide necessary
A 2-D liquid separations/mass mapping method for interlysate comparison of ovarian cancers.
Journal:Anal Chem
PubMed ID:11985308
A two-dimensional liquid phase separation of proteins from whole cell lysates coupled on-line to an electrospray-ionization time-of-flight (ESI-TOF) mass spectrometer (MS) is used to map the protein content of ovarian surface epithelial cells (OSE) and an ovarian carcinoma-derived cell line (ES2). The two dimensions involve the use of liquid isoelectric
3 total citations