Pierce™ Coomassie Brilliant Blue Dyes
Pierce™ Coomassie Brilliant Blue Dyes
Pierce™ Coomassie Brilliant Blue Dyes
Pierce™ Coomassie Brilliant Blue Dyes
Thermo Scientific™

Pierce™ Coomassie Brilliant Blue Dyes

Die Farbstoffe Thermo Scientific Pierce Coomassie Brilliant Blue bestehen aus einer der häufigsten Formen des Coomassie-Farbstoffs, der ein Hauptbestandteil verschiedener kolorimetrischer Proteingel-Färbmittel ist.
Have Questions?
Ansicht ändernbuttonViewtableView
KatalognummerFärbemitteltyp
20279Brilliant Blue G-250 Dye
20278Brilliant Blue R-250 Dye
Katalognummer 20279
Preis (EUR)
191,00
Each
-
Zum Warenkorb hinzufügen
Färbemitteltyp:
Brilliant Blue G-250 Dye
Großbestellung oder individuelle Größe anfordern
Preis (EUR)
191,00
Each
Zum Warenkorb hinzufügen
Ask our AI about this Product

Die Farbstoffe Thermo Scientific Pierce Coomassie Brilliant Blue bestehen aus einer der häufigsten Formen des Coomassie-Farbstoffs, der ein Hauptbestandteil verschiedener kolorimetrischer Proteingel-Färbmittel ist. Die Farbstoffe Coomassie R-250 und G-250 sind zwei chemische Formen einer disulfonierten Triphenylmethanverbindung, die häufig für Färbungen zum Nachweis von Proteinen in der Gelelektrophorese und Bradford-Assay-Reagenzien zur Proteinquantifizierung verwendet wird. Der R-250-Form (rot getönt) fehlen zwei Methylgruppen, die in der G-250-Form (grün getönt), die auch als kolloidaler Coomassie-Farbstoff bezeichnet wird, vorhanden sind.

Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation. The R-250 (red-tinted) form lacks two methyl groups that are present in the G-250 (green-tinted) form, which is also called colloidal coomassie dye.

Typically, coomassie gel stains and protein assay reagents are formulated as very acidic solutions in 25 to 50% methanol. In acidic conditions, the dye binds to proteins primarily through basic amino acids (primarily arginine, lysine, and histidine), and the number of coomassie dye ligands bound to each protein molecule is approximately proportional to the number of positive charges found on the protein. Protein binding causes the dye to change from reddish-brown to bright blue (absorption maximum equals 595 nm).

Features include:
Easy detection—develops intensely colored complexes with proteins
High sensitivity—can determine as little as 0.5 μg/cm2 of protein present in a gel matrix
Reversible staining—anion of Coomassie Brilliant Blue dye formed in the acidic staining medium combines with the protonated amino groups of proteins by electrostatic interaction; resulting complex is reversible under proper conditions
Differentiation between bound and unbound dye—when dissolved in 0.01 M citrate buffer at pH 3.0, has an absorption maximum at 555 nm; protein-dye complex is characterized by a peak slightly broader than that of free dye with a maximum at 549 nm

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DetektionsstelleIn-Gel-Detektion, In-Solution-Detektion, In-Blot-Detektion
NachweisverfahrenKolorimetrisch
ProduktliniePierce
ProdukttypProteingel-Färbemittel
Menge50 g
FärbemitteltypBrilliant Blue G-250 Dye
ZielmolekülProtein
Marker oder FarbstoffCoomassie
Unit SizeEach
Inhalt und Lagerung
Bei Raumtemperatur lagern.
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Abbildungen

Customers who viewed this item also viewed



Dokumente und Downloads

Zertifikate

Chargen-Nr.Certificate TypeDateCatalog Number(s)
3211872Certificate of Analysis30. Apr. 202520278
AC4587471Certificate of Analysis25. März 202520279
ZL4495481Certificate of Analysis21. Dez. 202420279
ZH402435Certificate of Analysis29. Aug. 202420278
ZH402435ACertificate of Analysis29. Aug. 202420278
5 Ergebnisse angezeigt, oben nach einem bestimmten Zertifikat suchen

Sicherheitsdatenblätter

Geben Sie die Katalognummer, den Namen oder Link frei.

1x1 image pixel for data collection