Primary Rat Cortex Neurons

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Gibco™

Primary Rat Cortex Neurons

Gibco Primary Rat Cortex Neurons are isolated and cryopreserved from day-18 rat embryos. A flexible, ready-to-use and quality alternative toRead more

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Catalog Number	Quantity
A1084001	1 x 10⁶ cells
A1084002	4 x 10⁶

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Catalog number A1084001

Price (USD)

365.00

Each

In stock

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Quantity:

1 x 10⁶ cells

Price (USD)

365.00

Each

Add to cart

Gibco Primary Rat Cortex Neurons are isolated and cryopreserved from day-18 rat embryos. A flexible, ready-to-use and quality alternative to fresh neurons, each vial contains 1 million cells per mL. These neurons consistently yield post-thaw viability in the range of 50–80% and have high neuronal purity.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Donor AttributesNormal

No. of Cells1 x 10^6

Product TypeRat Cortex Neuron

Quantity1 x 10⁶ cells

Tested ForSterility, Viability, Mycoplasma

AgeFetal

Cell TypeNeural Cells

Culture TypeMammalian Cell Culture

FormCryopreserved

SpeciesRat

TissueCortex

Unit SizeEach

Contents & Storage

Store in liquid nitrogen, vapor-phase.

Why does the manual for primary rat neurons have the instruction "Do not expose neurons to air at any time"?

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Frequently asked questions (FAQs)

What is the difference between Primary Rat Cortex Neurons, Sprague Dawley and Primary Rat Cortex Neurons?

These neurons differ in the rat strain from which they were isolated: Sprague Dawley vs. Fisher 344. These neurons don't expand anymore. They just grow neurites. The manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0001574-PrimaryRatCortex-HippocampusNeurons-UG.pdf) has instructions for culturing the cells. You can use Neurobasal Plus/B-27 Plus/GlutaMAX to culture them, or just Neurobasal/B-27/GlutaMAX. However, the former combination provides much superior performance than the latter and can maintain neurons longer.

I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What cell types will Poly-D-Lysine support as an attachment matrix?

The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can primary rat neurons be cryopreserved again after they are cultured?

Primary rat neurons cannot be cryopreserved again because they will not revive.

Can primary rat neurons proliferate?

No, primary rat neurons do not proliferate.

View all Primary Rat Cortex Neurons FAQs

Videos

EVOS M7000 microscope Z-stack of rat cortical neurons using a 20X S-Apo objective, DAPI, GFP and RFP LED cubes.

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Rat cortical neurons (A1084001) transfected with CellLight??? Golgi-RFP, BacMam 2.0 (C10593). At 48 hours cells were fixed and stained with anti-Tau (MN1000), followed by Goat anti-Mouse Alexa Fluor Plus 488 (A32723) and NucBlue??? Fixed Cell ReadyProbes??? Reagent (R37606). Cells were imaged on an M7000 (AMF7000) using an Olympus 20X objective (AMEP4734) with DAPI (AMEP4650), GFP (AMEP4651) and RFP (AMEP4652) LED cubes.