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Gibco™
CTS™ TrypLE™ Select Enzyme
TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation. The GIBCO™ CTS™Read more
| Catalog Number | Quantity |
|---|---|
| A1285901 | 100 mL |
Catalog number A1285901
Price (EUR)
54,00
Each
-
Quantity:
100 mL
Price (EUR)
54,00
Each
TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation. The GIBCO™ CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.
• GIBCO™ TrypLE™ Select ™ CTS™ enables you to reduce your burden in qualifying reagents during your transition from research to clinical applications.
• Non-animal or human origin
• Reduced risk of viral or prion contamination
• Removal kinetics and recovery viability comparable to porcine trypsin
• Demonstration of activity on multiple cell lines, including strongly attached lines
• Stable at room temperature (15° to 30°C) for up to 24 months.
• Manufactured using dedicated, autoclaved or disposable equipment.
TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation. This material is used for the dissociation of attachment-dependent cell lines from plasticware. TrypLE Select has demonstrated the ability to dissociate cells cultured both in serum-free and serum supplemented systems. The product is provided as a 1X stock solution, formulated in DPBS with 1mM EDTA. The concentration of rProtease is proprietary to
Invitrogen Corp.
GIBCO™ CTS™ products are of high quality, and are supplied with harmonized documentation such as Certificates of Analysis, Certificates of Origin, and access to authorization letters for our Drug Master Files, as appropriate. The GIBCO™ CTS™ product line enables you to reduce your burden in qualifying reagents during your transition from research applications to clinical applications.
For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals.
Specifications
Cell TypeMammalian Cells
Concentration1X
For Use With (Application)Cell and Gene Therapy Research, Development, and Manufacturing
FormatBottle(s)
Green FeaturesSustainable packaging
Manufacturing QualityISO 13485, MDSAP, FDA-registered, 21 CFR 820
Quantity100 mL
Shelf Life24 months from date of manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
FormLiquid
Product TypeCell Culture Dissociation Reagent
SterilitySterile-filtered
Without AdditivesNo Phenol Red
Unit SizeEach
Contents & Storage
Store at room temperature.
Media Formulations
Have questions about this product? Ask our AI assisted search.
What reagents do you offer for cell dissociation, and what are the main differences between them?
How can I assess residual TrypLE reagent remaining after my downstream processing steps?
Is there an upper limit for the amount of time cells can be incubated with TrypLE Express Enzyme, TrypLE Select Enzyme, or CTS TrypLE Select Enzyme for dissociation?
What is the difference between TrypLE Express Enzyme, TrypLE Select Enzyme, and CTS TrypLE Select Enzyme?
For your TrypLE products, how does the enzyme get inactivated?
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Example CTS™ Product COA - page 2
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Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3205879Certificate of AnalysisJun 06, 2025A1285901
3118372Certificate of AnalysisMay 24, 2025A1285901
3118370Certificate of AnalysisApr 27, 2025A1285901
2928578Certificate of AnalysisMar 28, 2025A1285901
2928576Certificate of AnalysisFeb 27, 2025A1285901
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Safety Data Sheets
SDSScientific Resources
Product Information
Limited Use Label Licenses (LULL)
'
Notice to Purchaser: The purchase of this product conveys to the purchaser the limited, non- transferable right to use the purchased amount of the product to (a) perform internal research for the sole benefit of the purchaser; (b) manufacture protein (or other biological material) for resale; and (c) perform research or manufacturing services conducted by the purchaser on a fee for service or contract basis for or on behalf of third parties. However, the purchaser may transfer this product, its components, or materials made using this product to a third party (including contract research/manufacturing organizations), provided that each such third party agrees in writing to use such product, components, or materials solely on behalf of the purchaser, and such third party is restricted from further transferring any such product, components, or materials to any individual or entity other than the purchaser. No additional rights are granted. By purchasing this product, the purchaser agrees not to: (1) resell the product in any form; (2) use the product as a therapeutic agent or diagnostics test component; (3) reverse engineer the product or cause the product to be reverse engineered; or (4) use the product for purposes other than what is indicated in this Limited Use Label License. Life Technologies is not aware of Intellectual Property ("IP") that would be infringed by the manufacture or sale of its products. Customers are urged to perform an IP search and analysis specific to their manufacturing processes and target biologic products. Should that analysis require information about Life Technologies products, Life Technologies will provide to the customer's IP counsel the information necessary to analyze any relevant IP in a manner that protects Life Technologies proprietary information. The purchaser is responsible for obtaining all regulatory approvals necessary for any therapeutic or diagnostic use of the protein (or biological material) manufactured using this product. For information on obtaining additional rights, please contact outlicensing@thermofisher.com, Licensing and Commercial Supply, Thermo Fisher Scientific, 5823 Newton Drive, Carlsbad, CA, 92008, United States.
'Frequently asked questions (FAQs)
The Gibco Cell Therapy Systems (CTS) portfolio of cell and gene therapy products are GMP manufactured, safety tested, and backed by regulatory documentation to support your transition from discovery through clinical and commercial manufacturing. Through our CTS solutions, we are committed to helping customers streamline therapeutic development, minimize risk, and ease the burden on their quality systems. Learn more here.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
We offer the Residual TrypLE ELISA Kit (Cat. No. A6533101) that can specifically detect the recombinant protease (rProtease) in TrypLE reagent.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
There is no upper limit for TrypLE enzyme incubation. You can incubate your cells with TrypLE longer to detach cells. 30-45 min at 37 degrees C will be usually fine. Or you can consider using TrypLE Select Enzyme (10X) (Cat. No. A1217701 or A1217702 or A1217703) or CTS TrypLE Select Enzyme (Cat. No. A4738001) depending upon the application.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Our TrypLE enzymes are very gentle, and there is no need to use serum or individual proteins to inactivate the enzyme. The TrypLE enzyme activity is inactivated by dilution using the following procedure:
After cell dissociation, transfer the cell suspension to a 15-mL conical tube. Centrifuge at 100 x g for 5-10 min to remove residual enzyme activity. Discard the supernatant and resuspend the cell pellet in 2-5 mL of pre-warmed complete medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
All the above products contain rProtease, a non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation.
The TrpLE Express, TrypLE Select, and CTS TrypLE Select products differ in their production and testing methods and in the documentation provided.
TrypLE Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and is intended for Research Use/Further Cell Culture Manufacturing.
TrypLE Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures.
CTS TrypLE Select is manufactured under scalable cGMP conditions using dedicated equipment and is designed for clinical applications. This product has a Drug Master File on record and is intended for Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. Not intended for direct administration into humans or animals.
To read more about TrypLE and cell release times using this reagent, please go here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).
Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.
Citations & References (1)
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Citations & References
Abstract
An effective freezing/thawing method for human pluripotent stem cells cultured in chemically-defined and feeder-free conditions.
Journal:
PubMed ID:25973330
Culturing human Pluripotent Stem Cells (hPSC)s in chemically defined medium and feeder-free condition can facilitate metabolome and proteome analysis of culturing cells and medium, and reduce regulatory concerns for clinical application of cells. And in addition, if hPSC are passaged and cryopreserved in single cells it also facilitates quality control
1 total citations