What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies. Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol 1 . 1. Pellerin, et al., Bioengineering, published in 2021.Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center .

Fetal Bovine Serum, exosome-depleted

Certificates

SDS

Gibco™

Fetal Bovine Serum, exosome-depleted

Gibco™ Exosome-Depleted FBS streamLines and standardizes cell culture workflows for exosome research. It is ready to use and performance tested to help save time and enable optimal exosome preparations.

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Catalog Number	Quantity	Format
A2720803	50 mL	One Shot
A2720801	500 mL	Bottle

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Catalog number A2720803

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247.00

Each

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50 mL

Format:

One Shot

Price (USD)

247.00

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Lowest exosome background
Enables consistent results: each lot tested for cell culture performance
No preparation needed: ultracentrifugation is not required

Exosome-Depleted FBS has been depleted of ≥90% of endogenous exosomes and is intended for use in cell culture exosome isolation assays. Every lot is tested for exosome depletion and culture performance, and our standard quality control assays are conducted to help ensure optimal and consistent results. This ready-to-use FBS for cell culture exosome isolations helps eliminate the risks associated with FBS reagent preparation by ultracentrifugation, for optimal assay results and increased experimental throughput.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

Specifications

Product LineOne Shot™

Shipping ConditionFrozen

SpeciesBovine

AgeFetal

Country of OriginUnited States

FormLiquid

FormatOne Shot

Product TypeFetal Bovine Serum

Quantity50 mL

Serum TreatmentExosome-depleted

SterilitySterile

Unit SizeEach

Contents & Storage

Storage conditions: ≤-10°C
Shipping conditions: Frozen

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Documents & Downloads

Certificates

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Lot #Certificate TypeDateCatalog Number(s)

U3151924RPCertificate of AnalysisMay 25, 2025A2720801, A2720803

U3196886RPCertificate of AnalysisMay 23, 2025A2720801, A2720803

U3189883RPCertificate of AnalysisMay 18, 2025A2720801, A2720803

U3151921RPCertificate of AnalysisMay 11, 2025A2720801, A2720803

U3151918RPCertificate of AnalysisApr 27, 2025A2720801, A2720803

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Safety Data Sheets

SDS

Scientific Resources

Brochure: Gibco Sera Guide

Brochure: One Shot FBS 50 mL Bottle

Flyer: Gibco FBS FAQs (frequently asked questions)

Frequently asked questions (FAQs)

Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol¹.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Yes, the material undergoes USP sterility testing, which includes mycoplasma testing.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Exosome-Depleted FBS is made from Gibco FBS (qualified, US-origin) in the Value category.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Serum can be kept at 2-8 degrees C for up to 4 weeks.

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Yes, we also offer a 10 X 50 mL package, which is equivalent to a 500 mL bottle of FBS.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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