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Pro-Detect™ Rapid Myc Competitive Assay Kit
The Thermo Scientific Pro-Detect Rapid Myc Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detectionRead more
| Catalog Number | Quantity |
|---|---|
| A38512 | 10 Assays |
Catalog number A38512
Price (USD)
146.00
Each
In stock
Quantity:
10 Assays
Price (USD)
146.00
Each
The Thermo Scientific Pro-Detect Rapid Myc Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of Myc (sequence: EQKLISEEDL)-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to Myc tag to provide a qualitative detection within ten minutes. This assay can be used to detect Myc-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.
Features of the Pro-Detect Rapid competitive assay kits:
• Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
• Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for qualitative results within 5-10 minutes
• Sensitive—detect protein tags in supernatant or cell lysate samples down to low μg/mL concentrations
• Specific—assay does not cross-react with common lysis and protein preparation reagents
• Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
• Long shelf life—stable for at least one year at 4°C
Each Pro-Detect Rapid competitive assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. In the presence of target protein tag, the dye-bound conjugated antibodies will bind to the tagged protein present in the sample instead of the protein tag bound at the test lines. Therefore, in a positive result, a competitive lateral flow assay will show decreasing intensity of the visible test lines until all test lines have disappeared. When there is no target protein tag present, the dye-bound conjugated antibodies specific to the target will bind at the test line(s), resulting in visual test lines in a negative result.
Features of the Pro-Detect Rapid competitive assay kits:
• Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
• Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for qualitative results within 5-10 minutes
• Sensitive—detect protein tags in supernatant or cell lysate samples down to low μg/mL concentrations
• Specific—assay does not cross-react with common lysis and protein preparation reagents
• Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
• Long shelf life—stable for at least one year at 4°C
Each Pro-Detect Rapid competitive assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. In the presence of target protein tag, the dye-bound conjugated antibodies will bind to the tagged protein present in the sample instead of the protein tag bound at the test lines. Therefore, in a positive result, a competitive lateral flow assay will show decreasing intensity of the visible test lines until all test lines have disappeared. When there is no target protein tag present, the dye-bound conjugated antibodies specific to the target will bind at the test line(s), resulting in visual test lines in a negative result.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayLateral Flow Rapid Assay
Detectable AnalytesMyc (EQKLISEEDL)-tagged proteins
For Use With (Application)Lateral Flow Rapid Assay
Product LinePro-Detect™
Product TypeAssay
Quantity10 Assays
Shipping ConditionWet Ice
SpecificityProtein tag
Sufficient For10 Assays
TargetProtein
Detection MethodChromogenic
Unit SizeEach
Contents & Storage
• 10 Rapid Assay Strips
• Sample Diluent, 15 mL
Sufficient for 10 assay determinations
Store at 2–8°C.
• Sample Diluent, 15 mL
Sufficient for 10 assay determinations
Store at 2–8°C.
Have questions about this product? Ask our AI assisted search.
Are the Pro-Detect assay kits compatible with common detergent and salt concentrations used in protein purification/detection protocols?
How do I interpret the gradual disappearance of four lines in the Pro-Detect competition assay kits?
Are the results of Pro-Detect assay kits comparable to western blotting?
Using Pro-Detect assay kits, can I detect the protein of interest in a mixture of proteins such as a crude lysate?
I am using a Pro-Detect assay kit and cannot differentiate banding pattern between control lysates and lysates that are actually expressing proteins of interest. What is the problem?
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Product Information
Frequently asked questions (FAQs)
The Pro-Detect Rapid Antibody Isotyping Kit is a lateral-flow assay with high sensitivity for rapid determination of the immunoglobulin class, subclass, and light chain type of monoclonal antibodies. Two different formats of rapid isotyping kits are available. These include dipstick format and cassette-based format. One can use these kits to identify the isotype of a mouse, rat, or human antibody. Make sure to follow the recommended dilution guidelines.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
1) It is likely that there could be a substance interfering with the assay. Check the compatibility table on this page (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/pro-detect-rapid-assays.html), under “Detergent and salt compatibility limits”.
2) The expression level of the actual tagged protein is below the detectable range of the assay.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Yes, first, make sure that the protein of interest is tagged and that we offer a Pro-Detect assay kit that can detect that tag. Secondly, make sure that you follow the guidelines to detect proteins in crude lysate and if necessary increase or decrease the lysate concentration to bring the protein of interest within the detectable range of the assay.
Note that the guidelines for the recommended detection ranges reflect the concentration of the pure tagged protein in a given sample mixture.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
As the concentration of the tag in the protein sample increases, the number of lines will decrease until they all disappear. The concentration of the tagged proteins is inversely related to the number of lines appearing on the strip.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Yes. Western blotting is a widely used and accepted tool to identify a specific protein in a complex mixture. However, it is a time-consuming and labor-intensive process, with many preparative steps and time intervals to manage. The Pro-Detect rapid assays are able to provide comparable accuracy but with much shorter processing time (10-15 min) and less hands-on steps. For further information, please see the data shown on this page (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/pro-detect-rapid-assays.html) under “Results are comparable to western blotting”.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.