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Invitrogen™
BacLight™ RedoxSensor™ Green Vitality Kit
El reactivo verde RedoxSensor™ incluido en el kit de vitalidad Bac Light™ RedoxSensor™ Green es un indicador de la actividadMás información
| Número de catálogo | Cantidad |
|---|---|
| B34954 | 200 kit |
Número de catálogo B34954
Precio (EUR)
980,00
Each
-
Cantidad:
200 kit
Precio (EUR)
980,00
Each
El reactivo verde RedoxSensor™ incluido en el kit de vitalidad Bac Light™ RedoxSensor™ Green es un indicador de la actividad de la reductasa bacteriana. Esta actividad de la reductasa es, a su vez, un marcador fiable para los cambios en la función de la cadena de transporte de electrones y para los cambios en la vitalidad que ocurren después del tratamiento con antibióticos. El reactivo RedoxSensor™ Green penetra en bacterias grampositivas y gramnegativas. Tras la reducción, el reactivo RedoxSensor™ Green producirá una señal verde fluorescente estable en 10 minutos compatible con las técnicas de fijación con formaldehído.
Consulte información adicional acerca de todos los ensayos microbiológicos para citometría de flujo.
Consulte información adicional acerca de todos los ensayos microbiológicos para citometría de flujo.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de célulaBacterias
Método de detecciónFluorescente
Tipo de coloranteRedoxSensor™ Green, PI
FormularioSolución
FormatoTubos, portaobjetos
Cantidad200 kit
Condiciones de envíoTemperatura ambiente
SolubilidadDMSO (dimetilsulfóxido)
EmissionPI: 490⁄635, RedoxSensor™ Green: 490⁄520
Para utilizar con (equipo)Microscopio de fluorescencia, Citómetro de flujo
Línea de productosBacLight™, RedoxSensor™
Tipo de productoKit de vitalidad verde
Unit SizeEach
Contenido y almacenamiento
Contiene un vial de RedoxSensor™ Green (200 μl, solución de 1 mm en DMSO), un vial de yoduro de propidio (300 μl, solución de 20 mm en DMSO), un vial de azida sódica (1 ml de una solución de 2 M en agua) y un vial de CCCP (400 μl, 5 mm en DMSO).
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Figuras
BacLight™ RedoxSensor™ Green Vitality Kit.
Chemical Structure
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Certificados
Buscar por número de lote o por número de lote parcial
N.º de loteCertificate TypeDateCatalog Number(s)
298954Certificate of Analysis26 mar 2025B34954
2800320Certificate of Analysis12 ene 2024B34954
2765710Certificate of Analysis14 dic 2023B34954
2729781Certificate of Analysis25 ago 2023B34954
2685291Certificate of Analysis05 jul 2023B34954
Se muestran 5 resultados, busque arriba un certificado específico
Hojas de datos de seguridad
SDSScientific Resources
Product Information
Manual de Molecular Probes®
Preguntas frecuentes
You can stain bacteria with a general stain such as BacLight Green Bacterial Stain (Cat. No. B35000) or BacLight Red Bacterial Stain (Cat. No. B35001). You can look at gram character (Cat. No. L7005), cell viability (Cat. Nos. L7007, L7012, and L13152), cell count (Cat. Nos. L34856 and B7277), and cell vitality. Cell vitality can be measured by membrane potential (Cat. No. B34950) or by metabolism (Cat. Nos. B34954 and B34956).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The excitation/emission of the RedoxSensor Green reagent, in reduced form, is 490/520 nm.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Citations & References (8)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Respiration response imaging for real-time detection of microbial function at the single-cell level.
Journal:Appl Environ Microbiol
PubMed ID:21075887
'The ability to detect specific functions of uncultured microbial cells in complex natural communities remains one of the most difficult tasks of environmental microbiology. Here we present respiration response imaging (RRI) as a novel fluorescence microscopy-based approach for the identification of microbial function, such as the ability to use C(1)
Extracellular reduction of uranium via Geobacter conductive pili as a protective cellular mechanism.
Journal:Proc Natl Acad Sci U S A
PubMed ID:21896750
'The in situ stimulation of Fe(III) oxide reduction by Geobacter bacteria leads to the concomitant precipitation of hexavalent uranium [U(VI)] from groundwater. Despite its promise for the bioremediation of uranium contaminants, the biological mechanism behind this reaction remains elusive. Because Fe(III) oxide reduction requires the expression of Geobacter''s conductive pili,
Fullerene water suspension (nC60) exerts antibacterial effects via ROS-independent protein oxidation.
Journal:Environ Sci Technol
PubMed ID:19031913
Buckminsterfullerene (C60) can form water suspensions (nC60) that exert toxic effects. While reactive oxygen species (ROS) generation has been implicated as the mechanism for mammalian cytotoxicity, we propose that nC60 exerts ROS-independent oxidative stress in bacteria, with evidence of protein oxidation, changes in cell membrane potential, and interruption of cellular
Oxygen consumption rates of bacteria under nutrient-limited conditions.
Journal:
PubMed ID:23770901
Many environments on Earth experience nutrient limitation and as a result have nongrowing or very slowly growing bacterial populations. To better understand bacterial respiration under environmentally relevant conditions, the effect of nutrient limitation on respiration rates of heterotrophic bacteria was measured. The oxygen consumption and population density of batch cultures
In vitro susceptibility and cellular uptake for a new class of antimicrobial agents: dinuclear ruthenium(II) complexes.
Journal:J Antimicrob Chemother
PubMed ID:22865383
To determine the in vitro susceptibility and cellular uptake for a series of dinuclear ruthenium(II) complexes [{Ru(phen)(2)}(2){µ-bb(n)}](4+) (Rubb(n)), and the mononuclear complexes [Ru(Me(4)phen)(3)](2+) and [Ru(phen)(2)(bb(7))](2+) against Staphylococcus aureus, methicillin-resistant S. aureus, Escherichia coli and Pseudomonas aeruginosa. The in vitro susceptibility was determined by MIC and MBC assays, and time-kill curve
8 total citations