Kit de comptage et de viabilité des bactéries LIVE/DEAD™ BacLight™, pour cytométrie en flux
Kit de comptage et de viabilité des bactéries LIVE/DEAD&trade; <i>Bac</i>Light&trade;, pour cytométrie en flux
Invitrogen™

Kit de comptage et de viabilité des bactéries LIVE/DEAD™ BacLight™, pour cytométrie en flux

Le kit de comptage et de viabilité des bactéries LIVE/DEAD™ BacLight™ permet aux chercheurs de distinguer et d’analyser quantitativement deAfficher plus
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RéférenceQuantité
L34856100 réactions
Référence L34856
Prix (EUR)
950,00
Each
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Quantité:
100 réactions
Prix (EUR)
950,00
Each
Ajouter au panier
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Le kit de comptage et de viabilité des bactéries LIVE/DEAD™ BacLight™ permet aux chercheurs de distinguer et d’analyser quantitativement de façon fiable les bactéries vivantes et mortes à l’aide d’un cytomètre en flux, même dans une population mélangée contenant divers types de bactéries. Ce kit utilise un mélange de deux colorants d’acides nucléiques, le colorant vert fluorescent SYTO™ 9 et le colorant rouge fluorescent à l’iodure de propidium, pour déterminer la viabilité, et une suspension étalonnée de microsphères pour des mesures précises du volume des échantillons.

Consultez des informations complémentaires sur tous les dosages de cytométrie de flux en microbiologie.
Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.
Spécifications
Méthode de détectionFluorescence
FormatTube
Marqueur ou colorantSYTO™ 9, iodure de propidium
Gamme de produitsBacLight™, LIVE/DEAD™
Quantité100 réactions
Conditions d’expéditionTempérature ambiante
TypeKit de comptage et de viabilité des bactéries
Longueur d’ondesSYTO™ 9 : 485/498, IP : 535/617
Unit SizeEach
Contenu et stockage
Contient 1 flacon de coloration d’acides nucléiques SYTO™ 9 (200 µl, 3,34 mM dans le DMSO), 1 flacon d’iodure de propidium (200 µl, 20 mM dans le DMSO) et 1 flacon d’un étalon de microsphère (1,0 ml de microsphères de 6,0 µm de diamètre).

Conserver au réfrigérateur (entre 2 et 8°C) et le protéger de la lumière.
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Figures

Spectres de fluorescence

Fluorescence spectra

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Certificats

Numéro de lotCertificate TypeDateCatalog Number(s)
2456936Certificate of Analysis06 févr. 2025L34856
3096426Certificate of Analysis22 déc. 2024L34856
2800323Certificate of Analysis09 janv. 2024L34856
2585787Certificate of Analysis05 mai 2023L34856
2351982Certificate of Analysis19 juil. 2021L34856
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Safety Data Sheets

Foire aux questions (FAQ)

SYTO 9 dye will enter all cells, live or dead. PI only enters cells with compromised membranes (dead cells or damaged cells). First, perform single color staining and examine under both filter sets. Longpass filters or the use of too much dye may result in excessive bleedthrough of the green dye emission into the red channel and the emission of PI in the green channel. Use narrower bandpass filters if possible, and use lower concentrations of the dye. Some live cells may take up PI by engulfment; avoid extended incubation times with the dye. Apply PI to a live cell culture and optimize incubation times to limit engulfment.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Oxygen content should not affect the binding of propidium iodide and SYTO 9 to nucleic acids. SYTO 9 will label all cells, and propidium iodide will label only dead cells or cells with a compromised membrane.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Unfortunately, no. SYTO 9 will label the nuclei of live or dead cells, including the eukaryotic cells. Propidium iodide is cell impermeant, and will only enter dead cells. If the eukaryotic cells are dead, they will label with propidium iodide as well. If the eukaryotic cells are alive, propidium iodide will not be able to enter and thus will not label the bacteria inside, whether the bacteria are alive or dead. We are not aware of any way to do a viability assay of bacteria once they have been engulfed by cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

The green dye in the kit will label all the cells as it is a cell-permeant nucleic acid stain. The red dye is not cell permeant, and will only stain the cells with compromised membranes (dead cells). Therefore, any cells with red signal will be considered dead. It is possible that you will have some cells that are only red, some that are red and green, and some that are only green. Sometimes the red dye will displace the green dye. In any case, any red cells are dead.

Also, the green dye emission may bleed through into the red channel. Do a single-color staining and examine under both green and red filter sets to determine the level of bleedthrough. To avoid this bleedthrough, use a lower concentration of dye, and, if possible, use narrow bandpass filters.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

There are several options. We have two fluorescence based kits that are useful for bacterial counting: Live/Dead BacLight Bacterial Viability and Counting Kit, for flow cytometry (Cat. No. L34856) and Bacteria Counting kit, for flow cytometry (Cat. No. B7277). Another option is the Flow Cytometry Sub-micron Particle Size Reference Kit (Cat. No. F13839).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (122)

Citations et références
Abstract
Authors:
Journal:
PubMed ID:16517648
Microbial activity in soils following steam treatment.
Authors:Richardson RE,James CA,Bhupathiraju VK,Alvarez-Cohen L
Journal:Biodegradation
PubMed ID:12521292
Steam enhanced extraction (SEE) is an aquifer remediation technique that can be effective at removing the bulk of non-aqueous phase liquid (NAPL) contamination from the subsurface, particularly highly volatile contaminants. However, low volatility compounds such as polynuclear aromatic hydrocarbons (PAHs) are less efficiently removed by this process. This research evaluated ... More
Real-time PCR analysis of Vibrio vulnificus from oysters.
Authors:Campbell MS, Wright AC
Journal:Appl Environ Microbiol
PubMed ID:14660359
Vibrio vulnificus is an opportunistic human pathogen commonly found in estuarine environments. Infections are associated with raw oyster consumption and can produce rapidly fatal septicemia in susceptible individuals. Standard enumeration of this organism in shellfish or seawater is laborious and inaccurate; therefore, more efficient assays are needed. An oligonucleotide probe ... More
CFTR regulates phagosome acidification in macrophages and alters bactericidal activity.
Authors:Di A, Brown ME, Deriy LV, Li C, Szeto FL, Chen Y, Huang P, Tong J, Naren AP, Bindokas V, Palfrey HC, Nelson DJ
Journal:Nat Cell Biol
PubMed ID:16921366
'Acidification of phagosomes has been proposed to have a key role in the microbicidal function of phagocytes. Here, we show that in alveolar macrophages the cystic fibrosis transmembrane conductance regulator Cl- channel (CFTR) participates in phagosomal pH control and has bacterial killing capacity. Alveolar macrophages from Cftr-/- mice retained the ... More
Ethidium monoazide for DNA-based differentiation of viable and dead bacteria by 5'-nuclease PCR.
Authors:Nogva HK, Drømtorp SM, Nissen H, Rudi K
Journal:Biotechniques
PubMed ID:12703305
'PCR techniques have significantly improved the detection and identification of bacterial pathogens. Even so, the lack of differentiation between DNA from viable and dead cells is one of the major challenges for diagnostic DNA-based methods. Certain nucleic acid-binding dyes can selectively enter dead bacteria and subsequently be covalently linked to ... More
122 total citations

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