CellTrace™ Calcein Violet, AM, for 405 nm excitation - Special Packaging
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CellTrace™ Calcein Violet, AM, for 405 nm excitation - Special Packaging

CellTrace™ calcein violet AM is an optimal dye for distinguishing live cells through the presence of ubiquitous intracellular esterase activity.Read more
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Catalog NumberQuantity
C34858Promo Image20 x 25 μg
Catalog number C34858
Price (USD)
460.65
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614.00
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20 x 25 μg
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Price (USD)
460.65
Online Exclusive
614.00
Save 153.35 (25%)
Each
Add to cart
Ask our AI about this Product
CellTrace™ calcein violet AM is an optimal dye for distinguishing live cells through the presence of ubiquitous intracellular esterase activity. The enzymatic conversion of the virtually non-fluorescent cell-permeant calcein violet AM to the intensely fluorescent calcein violet (ex/em 400/452 nm) can be easily detected with the violet laser, allowing other laser lines to be used for conventional fluorochromes.

View a selection guide for all Nonfixable Viability Dyes for Flow Cytometry.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye Typecalcein violet
Excitation Wavelength Range400⁄452
For Use With (Equipment)Flow Cytometer
FormatTube(s)
Product LineCellTrace™
Quantity20 x 25 μg
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Product TypeDye
Unit SizeEach
Contents & Storage
Contains 20 vials of calcein violet AM (25 μg per vial). Store in freezer (-5 to -30°C) and protect from light.
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Frequently asked questions (FAQs)

I need a general cytoplasmic stain that does not overlap with the GFP in my cells. What do you recommend?

Calcein AM, a green dye, is typically used as a general cytoplasmic stain, but not recommended with GFP-positive cells. For GFP-expressing cells there are other colors available: Calcein Blue AM, Calcein Violet AM, and Calcein Red-Orange AM. The retention time of these dyes in live cells is dependent upon the inherent properties of the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained two populations of cells, one with CellTracker Green and the other with CellTracker Red, but it looks like there may be crossover of the red dye to the green cells. What is going on?

One possibility is that there is spectral bleedthrough between the dyes. Be sure to check the single-color samples by imaging the red cells in green and imaging the green cells in red, using the optimal imaging settings for the other color. If you see bleedthrough with these controls, then you will have to reduce the dye label concentration to reduce the brightness of the dyes, or choose dyes that are farther apart spectrally. If the issue isn’t bleedthrough, another possibility is that the cells were not adequately washed after staining, allowing some unincorporated dye to remain and label the other cells after they were introduced. Extending washes and wash times should help with this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained my cells with Calcein, AM, but the signal went away after I fixed my cells. Why is this?

Calcein, AM diffuses into cells, the 'AM' moiety is cleaved by cellular esterases, and then the dye molecules are observed in the cytoplasm without binding to anything. This gives a 'whole cell' stain. It also means that the dyes are not crosslinked with aldehyde-based fixation and thus will be lost upon fixation. Additionally, any disruption of plasma membrane, such as with detergents or trypsinization, will lead to leakage of the dye from the cell.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I'm trying to stain my cells with CellTracker dyes or CFDA SE, but I'm not seeing much signal. What can I do?

First, make sure you aren’t staining in the presence of serum, since serum can have esterase activity that can prematurely cleave the AM group on these dyes, preventing entry into cells. After staining, it’s okay to return the cells to medium containing serum. After this, you can try increasing the concentration and label time to get a higher intensity.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I like how calcein dyes label the whole cell. How long can I track my cells with them, and can I fix them?

Calcein dyes diffuse into cells, the 'AM' moiety is cleaved by cellular esterases and then are observed in the cytoplasm without binding to anything. This provides a 'whole cell' label. Calcein dyes may be pumped out by normal cellular efflux mechanisms, sometimes within a very short time, especially for cell types that may exhibit drug resistance, unless the efflux is inhibited (such as with probenecid). The dyes are not crosslinked with aldehyde-based fixation, unlike protein-binding CellTracker dyes, and thus will be lost upon fixation. Additionally, any disruption of plasma membrane, such as with detergents or trypsinization, will lead to leakage of the dyes from the cell.

Find additional tips, troubleshooting help, and resources within our Cell Tracing and Tracking Support Center.

Fluorescence spectra

Fluorescence spectra

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Lot #Certificate TypeDateCatalog Number(s)
3101963Certificate of AnalysisNov 25, 2024C34858
2935413Certificate of AnalysisJun 28, 2024C34858
2663871Certificate of AnalysisJul 28, 2023C34858
2497568Certificate of AnalysisJul 12, 2022C34858
2403701Certificate of AnalysisOct 23, 2021C34858
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Citations & References (5)

Citations & References
Abstract
Toll-like receptor 2-mediated signaling requirements for Francisella tularensis live vaccine strain infection of murine macrophages.
Authors:Cole LE, Shirey KA, Barry E, Santiago A, Rallabhandi P, Elkins KL, Puche AC, Michalek SM, Vogel SN,
Journal:Infect Immun
PubMed ID:17517865
'Francisella tularensis, an aerobic, non-spore-forming, gram-negative coccobacillus, is the causative agent of tularemia. We reported previously that F. tularensis live vaccine strain (LVS) elicited strong, dose-dependent NF-kappaB reporter activity in Toll-like receptor 2 (TLR2)-expressing HEK293T cells and proinflammatory gene expression in primary murine macrophages. Herein, we report that F. tularensis ... More
Trogocytosis by Entamoeba histolytica contributes to cell killing and tissue invasion.
Authors:Ralston KS, Solga MD, Mackey-Lawrence NM, Somlata, Bhattacharya A, Petri WA,
Journal:
PubMed ID:24717428
'Entamoeba histolytica is the causative agent of amoebiasis, a potentially fatal diarrhoeal disease in the developing world. The parasite was named '
Bicarbonate and functional CFTR channel are required for proper mucin secretion and link cystic fibrosis with its mucus phenotype.
Authors:Gustafsson JK, Ermund A, Ambort D, Johansson ME, Nilsson HE, Thorell K, Hebert H, Sjövall H, Hansson GC,
Journal:J Exp Med
PubMed ID:22711878
'Cystic fibrosis (CF) is caused by a nonfunctional chloride and bicarbonate ion channel (CF transmembrane regulator [CFTR]), but the link to the phenomenon of stagnant mucus is not well understood. Mice lacking functional CFTR (Cftr?508) have no lung phenotype but show similar ileal problems to humans. We show that the ... More
Multiplexed labeling of viable cells for high-throughput analysis of glycine receptor function using flow cytometry.
Authors:Gilbert DF, Wilson JC, Nink V, Lynch JW, Osborne GW,
Journal:Cytometry A
PubMed ID:19184990
Flow cytometry is an important drug discovery tool because it permits high-content multiparameter analysis of individual cells. A new method dramatically enhanced screening throughput by multiplexing many discrete fixed cell populations; however, this method is not suited to assays requiring functional cellular responses. HEK293 cells were transfected with unique mutant ... More
Induction of heat shock proteins in cerebral cortical cultures by celastrol.
Authors:Chow AM, Tang DW, Hanif A, Brown IR
Journal:Cell Stress Chaperones
PubMed ID:22865541
Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (ALS) are 'protein misfolding disorders' of the mature nervous system that are characterized by the accumulation of protein aggregates and selective cell loss. Different brain regions are impacted, with Alzheimer's affecting cells in the cerebral cortex, Parkinson's targeting dopaminergic cells in the ... More
5 total citations

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