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Thermo Scientific™
Eco31I (BsaI) (10 U/μL)
5' G G T C T C N1↓ 3'3' C C A G A G N5↑ 5'Thermo Scientific Eco31I (BsaI)Read more
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| Catalog Number | Quantity |
|---|---|
| ER0291 | 1,000 units |
| ER0292 | 5,000 units |
Catalog number ER0291
Price (USD)
100.65
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108.00Save 7.35 (7%)
Each
In stock
Quantity:
1,000 units
Price (USD)
100.65
Online Exclusive
108.00Save 7.35 (7%)
Each
| 5' | G | G | T | C | T | C | N1 | ↓ | 3' | |||
| 3' | C | C | A | G | A | G | N5 | ↑ | 5' |
Thermo Scientific Eco31I (BsaI) restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in G buffer (Isoschizomers: BsaI, Bso31I, BspTNI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.
Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.
Features
• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
Applications
• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
Note: For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible Buffer10X Buffer G
Product TypeRestriction Enzyme
Quantity1,000 units
Concentration10 U/μL
EnzymeEco31I (BsaI)
Methylation SensitivityCpG Methylation-Sensitive, Not CpG Methylation-Sensitive, Not Dam Methylation-Sensitive
Optimal Reaction Temperature37°C
Research CategoryScarless Cloning
Sensitive to Heat InactivationYes
Type IIS REYes
Unit SizeEach
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
For optimal results with fast reaction and 100% buffer compatibility, we highly recommend using FastDigest restriction enzymes in double digestion. In certain cases however, it may be possible to perform double digestion using a mix of Thermo Scientific conventional and Fastdigest restriction enzymes. For specific recommendations, please contact our technical service with detailed information about the enzymes and DNA template you plan to use.
We recommend only 2 µl 10X Buffer in digestion of unpurified PCR products in 30 ul since salts and ions from the PCR reaction would be carried over to the digestion reaction.
Find additional tips, troubleshooting help, and resources within our Restriction Enzyme Cloning Support Center.
Star activty may be contributed by:
• Prolonged incubation
• High enzyme concentration
• High glycerol concentration (usually 5% or higher)
• Small reaction volume
Find additional tips, troubleshooting help, and resources within our Restriction Enzyme Cloning Support Center.
Unexpected cleavage patterns may be caused by the following reasons:
• Star activity of the restriction enzyme: Make sure to follow the reaction recommendations as specified in the protocol. Star activity may be improved by changing several key factors such as decreasing the reaction time, increasing the reaction volume, and decreasing the enzyme amount.
• Partial or incomplete cleavage (incomplete restriction reaction): Efficiency of the enzyme can be improved by adding more enzyme, prolonging the reaction time, or purifying DNA samples to remove inhibitory contaminants.
• Contamination with non-specific endonucleases: Non-specific endonucleases may be introduced to the DNA sample and/or the enzyme from improper handling, pipetting, etc.
•Improper reaction setup: Mix the digestion reaction thoroughly.
Find additional tips, troubleshooting help, and resources within ourRestriction Enzyme Cloning Support Center.
The main reason for DNA cleavage reaction failure is the presence of contaminating inhibitors in the template DNA (for example: phenol, chloroform, detergents, ethanol, excess salts, EDTA, etc.). The best way to troubleshoot is to perform control reactions:
1) negative control (experimental DNA in the reaction buffer without the restriction enzyme) to access degradation of DNA by contaminants in the DNA template and/or reaction buffer
2) positive control reaction I (digestion of highly pure control DNA with the restriction enzyme) to access reaction conditions and enzyme activity
3) positive control reaction II (highly pure control DNA + experimental DNA + Restriction Enzyme) to access possible issues with the experimental DNA.
In addition, please check for sensitivity of the restriction enzymes to template DNA methylation.
Find additional tips, troubleshooting help, and resources within our Restriction Enzyme Cloning Support Center.