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DNase I, RNase-free (1 U/μL)
DNase I, RNase-free (1 U/μL)
Thermo Scientific™

DNase I, RNase-free (1 U/μL)

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono-Read more
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Catalog NumberQuantity
EN05211,000 units
Catalog number EN0521
Price (USD)
76.65
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91.75
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Price (USD)
76.65
Online Exclusive
91.75
Save 15.10 (16%)
Each
Add to cart
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Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.

The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions.

In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently in a statistically random fashion. In the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides.

Highlights

• Recombinant enzyme
• Purified from non-animal host with a lower level of intrinsic RNases

Applications

• Preparation of DNA-free RNA
• Removal of template DNA following in vitro transcription
• Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR
• DNA labeling by nick-translation in conjunction with DNA Polymerase I
• Studies of DNA-protein interactions by DNase I, RNase-free footprinting
• Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used

Note

DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible BufferReaction Buffer
Product TypeDNase I RNase Free
Quantity1,000 units
Concentration1 U/μL
EnzymeDNase
Unit SizeEach
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Lot #Certificate TypeDateCatalog Number(s)
3259987Certificate of AnalysisJun 13, 2025EN0521
3254186Certificate of AnalysisJun 04, 2025EN0521
3247234Certificate of AnalysisMay 26, 2025EN0521
3209117Certificate of AnalysisApr 14, 2025EN0521
3205292Certificate of AnalysisApr 07, 2025EN0521
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