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Nile Red
Nile Red
Invitrogen™

Nile Red

Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescentRead more
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Catalog NumberQuantity
N1142Promo Image25 mg
Catalog number N1142
Price (USD)
231.00
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Quantity:
25 mg
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Price (USD)
231.00
Each
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Nile red is used to localize and quantitate lipids, particularly neutral lipid droplets within cells. Nile red is almost nonfluorescent in water and other polar solvents but undergoes fluorescence enhancement and large absorption and emission blue shifts in nonpolar environments (Excitation/emission maxima ∼552/636 nm in methanol).

We recommend dissolving in high-quality anhydrous DMSO or DMF to attain a stock concentration up to 1 mM.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Excitation/Emission552/636 nm
For Use With (Equipment)Fluorescence Microscope
Molecular FormulaC20H18N2O2
Quantity25 mg
Recommended StorageStore at room temperature and protect from light.
Shipping ConditionRoom Temperature
Sub Cellular LocalizationCell Membranes & Lipids, Lysosomes
ColorRed
Physical FormSolid
Product TypeNile Red Stain
Unit SizeEach
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Figures

Fluorescence spectra

Fluorescence spectra

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Lot #Certificate TypeDateCatalog Number(s)
3192601Certificate of AnalysisJun 01, 2025N1142
3148291Certificate of AnalysisMar 31, 2025N1142
3135154Certificate of AnalysisJan 21, 2025N1142
3118575Certificate of AnalysisJan 04, 2025N1142
3023505Certificate of AnalysisAug 19, 2024N1142
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Citations & References (187)

Citations & References
Abstract
Developmental shift in the relative percentages of lung fibroblast subsets: role of apoptosis postseptation.
Authors:Awonusonu F,Srinivasan S,Strange J,Al-Jumaily W,Bruce MC
Journal:The American journal of physiology
PubMed ID:10516228
Global metabolic profiling of infection by an oncogenic virus: KSHV induces and requires lipogenesis for survival of latent infection.
Authors:Delgado T, Sanchez EL, Camarda R, Lagunoff M,
Journal:PLoS Pathog
PubMed ID:22916018
Like cancer cells, virally infected cells have dramatically altered metabolic requirements. We analyzed global metabolic changes induced by latent infection with an oncogenic virus, Kaposi's Sarcoma-associated herpesvirus (KSHV). KSHV is the etiologic agent of Kaposi's Sarcoma (KS), the most common tumor of AIDS patients. Approximately one-third of the nearly 200 ... More
Three-colour fluorescence immunohistochemistry reveals the diversity of cells staining for macrophage markers in murine spleen and liver.
Authors:Lloyd CM, Phillips AR, Cooper GJ, Dunbar PR,
Journal:J Immunol Methods
PubMed ID:18367204
'Macrophages have traditionally been identified in murine tissues using a small range of markers, typically F4/80, CD68 and CD11b. However many studies have suggested that substantial heterogeneity exists in macrophage populations, and no single marker, nor even pair of markers, can necessarily identify all the populations. Further, many of the ... More
Lipid-dependent bidirectional traffic of apolipoprotein B in polarized enterocytes.
Authors:Morel E, Demignot S, Chateau D, Chambaz J, Rousset M, Delers F
Journal:Mol Biol Cell
PubMed ID:14565984
'Enterocytes are highly polarized cells that transfer nutrients across the intestinal epithelium from the apical to the basolateral pole. Apolipoprotein B (apoB) is a secretory protein that plays a key role in the transepithelial transport of dietary fatty acids as triacylglycerol. The evaluation of the control of apoB traffic by ... More
Functional analysis of FSP27 protein regions for lipid droplet localization, caspase-dependent apoptosis, and dimerization with CIDEA.
Authors:Liu K, Zhou S, Kim JY, Tillison K, Majors D, Rearick D, Lee JH, Fernandez-Boyanapalli RF, Barricklow K, Houston MS, Smas CM,
Journal:Am J Physiol Endocrinol Metab
PubMed ID:19843876
'The adipocyte-specific protein FSP27, also known as CIDEC, is one of three cell death-inducing DFF45-like effector (CIDE) proteins. The first known function for CIDEs was promotion of apoptosis upon ectopic expression in mammalian cells. Recent studies in endogenous settings demonstrated key roles for CIDEs in energy metabolism. FSP27 is a ... More
187 total citations

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