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Invitrogen™
Dil Stain (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate ('DiI'; DiIC18(3)))
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporatedRead more
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| Catalog Number | Quantity |
|---|---|
| D3911 | 25 mg |
| D282 | 100 mg |
Catalog number D3911
Price (EUR)
499,65
Online Exclusive
536,00Save 36,35 (7%)
Each
-
Quantity:
25 mg
Price (EUR)
499,65
Online Exclusive
536,00Save 36,35 (7%)
Each
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orange/red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells. DiI is also available as a solution (Cat. No. V-22885), as a paste (Cat. No. N-22880) or as a solid (Cat. No. D-282).
Prepare stock solutions of lipophilic tracers in dimethyl formamide (DMF), dimethylsulfoxide (DMSO), or ethanol at 1 to 2.5 mg/mL.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorYellow
Detection MethodFluorescence
Emission565 nm
Excitation Wavelength Range549 nm
For Use With (Equipment)Fluorescence Microscope
Molecular Weight (g/mol)933.88
Quantity25 mg
Shipping ConditionRoom Temperature
Product TypeLiphophilic Tracer
SubCellular LocalizationCell Membranes, Lipids
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.
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Figures
Newborn mouse cochlea. DiI.
Neurons in ventrobasal nucleus of newborn mouse thalamus. DiI, DiD.
Chemical Structure
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3154627Certificate of AnalysisMay 18, 2025D3911
3135809Certificate of AnalysisMar 30, 2025D282
3096414Certificate of AnalysisNov 18, 2024D3911
3096413Certificate of AnalysisNov 06, 2024D282
2930831Certificate of AnalysisJul 17, 2024D282
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Frequently asked questions (FAQs)
This is expected. DiD (which is far-red fluorescent) is never as uniform as DiI (which is orange fluorescent). If uniformity is desired, try increasing the label time and concentration, but it still isn't likely to be as uniform as DiI. CellMask Deep Red Plasma Membrane stain is much more uniform and is about the same wavelength as DiD. However, if you intend to do cell tracking over days, CellMask stain has not been tried for that application.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
If the tracer you chose is a lipophilic dye and fix with methanol, the lipids are lost with the methanol. If you have to use methanol fixation then choose a tracer that will covalently bind to proteins in the neurons.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
DiI is a lipophilic dye that resides mostly in lipids in the cell, when cells are permeabilized with detergent or fixed using alcohol this strips away the lipid and the dye. If permeabilization is required CM-DiI can be used because this binds covalently to proteins in the membrane; some signal is lost upon fixation/permeabilization, but enough signal should be retained to make detection possible.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Citations & References (1179)
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Citations & References
Abstract
Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo.
Journal:Methods in molecular biology (Clifton, N.J.)
PubMed ID:19030810
Angiotensin II induces LOX-1, the human endothelial receptor for oxidized low-density lipoprotein.
Journal:Circulation
PubMed ID:10468518
Neural transplant staining with DiI and vital imaging by 2-photon laser-scanning microscopy.
Journal:Scanning Microsc Suppl
PubMed ID:9601539
We are developing a multielectrode silicon "neuroprobe" for maintaining a long-term, specific, two-way electrical interface with nervous tissue. Our approach involves trapping a neuron (from an embryonic rat hippocampus) in a small well with a stimulation/recording electrode at its base. The well is covered with a grillwork through which the
Cultured postnatal rat septohippocampal neurons change intracellular calcium in response to ethanol and nerve growth factor.
Journal:Brain Res
PubMed ID:9459553
Ethanol exposure affects cellular mechanisms involved in the regulation of calcium (Ca2+) homeostasis. Neurotrophins, such as nerve growth factor (NGF), stabilize intracellular Ca2+([Ca2+]i) during a variety of neurotoxic insults. In this study, changes in [Ca2+]i during treatment with ethanol and NGF were measured at the cell body of neurons using
Evaluation of a flow cytometric fluorescence quenching assay of phagocytosis of sensitized sheep erythrocytes by polymorphonuclear leukocytes.
Journal:Cytometry
PubMed ID:7875036
A number of reports have been published describing phagocytosis assays for flow cytometric analysis. In some of these, the fluorescence quenching technique has been used to discriminate between adherent and ingested particles. In this report, we have evaluated the efficacy of a quantitative fluorescence quenching technique with crystal violet and
1179 total citations