Suplemento B-27™ (50x), sin suero
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Suplemento B-27™ (50x), sin suero
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Gibco™

Suplemento B-27™ (50x), sin suero

El suplemento B-27 para el cultivo celular de neuronas es el más citado; es un suplemento optimizado sin suero queMás información
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Número de catálogoCantidad
1750404410 mL
17504001100 mL
Número de catálogo 17504044
Precio (USD)
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Cantidad:
10 mL
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El suplemento B-27 para el cultivo celular de neuronas es el más citado; es un suplemento optimizado sin suero que se utiliza para favorecer el crecimiento de baja o alta densidad y la viabilidad a corto o largo plazo de neuronas del hipocampo embrionarias, posnatales y adultas, así como otras neuronas del SNC. El suplemento B-27 sin vitamina A se suministra como un líquido 50X y se ha diseñado para su uso con medios Neurobasal o Neurobasal-A para realizar cultivos celulares de neuronas sin necesidad de una capa subyacente de astrocitos.

El suplemento B-27 es adecuado para su uso en la mayoría de las aplicaciones de células neuronales. Para otras aplicaciones, ofrecemos formulaciones alternativas del Suplemento B-27 y medios Neurobasal.

Para protocolos establecidos que usen el suplemento B-27, acceda a nuestro manual de protocolos de neurobiología Gibco.

Vea las publicaciones que utilizan productos de neurobiología Gibco en nuestra CellCite Gibco.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Tipo de célulaCélulas neuronales
Concentración50 X
Tipo de cultivoCultivo de células de mamífero
Línea de productosB-27™
Cantidad10 mL
Nivel de sueroSin suero
Duración de almacenamiento12 meses
Condiciones de envíoHielo seco
FuenteSin suero
Probado paraEndotoxinas, rendimiento, esterilidad, micoplasma
ClasificaciónSin suero
FormularioLíquido
Tipo de productoSuplemento de cultivo celular
EsterilidadEstéril
pHentre 6 y 8
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de – 5 a – 20°C) y proteger de la luz.
Formulaciones de medios
B-27 Serum-Free Supplement (50X) liquid
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Preguntas frecuentes

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which media system should I use for primary culture of P0- or P1-derived mixed hippocampal cells?

We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Suplemento B-27™ (50x), sin suero FAQs

Figuras

Primary rat Hippocampus neurons cultured in B-27 Supplement
Primary rat Hippocampus neurons cultured in B-27 Supplement
Rat cortical neurons cultured in Neurobasal Medium with B-27 Supplement
Rat cortical neurons cultured in Neurobasal Medium with B-27 Supplement
Primary rat cortex neurons cultured in B-27 Supplement
Primary rat cortex neurons cultured in B-27 Supplement

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Certificados

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N.º de loteCertificate TypeDateCatalog Number(s)
2954071Certificate of Analysis22 jun 202517504044, 17504001
2954081Certificate of Analysis22 jun 202517504044, 17504001
3135631Certificate of Analysis21 jun 202517504044, 17504001
2954065Certificate of Analysis03 may 202517504044, 17504001
3011455Certificate of Analysis23 abr 202517504044, 17504001
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Product Information

Citations & References (7)

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Citations & References
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
In vitro generation of human pluripotent stem cell derived lung organoids.
Authors:Dye BR, Hill DR, Ferguson MA, Tsai YH, Nagy MS, Dyal R, Wells JM, Mayhew CN, Nattiv R, Klein OD, White ES, Deutsch GH, Spence JR
Journal:
PubMed ID:25803487
'Recent breakthroughs in 3-dimensional (3D) organoid cultures for many organ systems have led to new physiologically complex in vitro models to study human development and disease. Here, we report the step-wise differentiation of human pluripotent stem cells (hPSCs) (embryonic and induced) into lung organoids. By manipulating developmental signaling pathways hPSCs ... More
Directed differentiation of human pluripotent stem cells into intestinal tissue in vitro.
Authors:Spence JR, Mayhew CN, Rankin SA, Kuhar MF, Vallance JE, Tolle K, Hoskins EE, Kalinichenko VV, Wells SI, Zorn AM, Shroyer NF, Wells JM
Journal:Nature
PubMed ID:21151107
Studies in embryonic development have guided successful efforts to direct the differentiation of human embryonic and induced pluripotent stem cells (PSCs) into specific organ cell types in vitro. For example, human PSCs have been differentiated into monolayer cultures of liver hepatocytes and pancreatic endocrine cells that have therapeutic efficacy in ... More
Axonal Transport Enables Neuron-to-Neuron Propagation of Human Coronavirus OC43.
Authors:Dubé M, Le Coupanec A, Wong AHM, Rini JM, Desforges M, Talbot PJ
Journal:J Virol
PubMed ID:29925652
Human coronaviruses (HCoVs) are recognized respiratory pathogens for which accumulating evidence indicates that in vulnerable patients the infection can cause more severe pathologies. HCoVs are not always confined to the upper respiratory tract and can invade the central nervous system (CNS) under still unclear circumstances. HCoV-induced neuropathologies in humans are ... More
Infection of bat and human intestinal organoids by SARS-CoV-2.
Authors:Zhou J, Li C, Liu X, Chiu MC, Zhao X, Wang D, Wei Y, Lee A, Zhang AJ, Chu H, Cai JP, Yip CC, Chan IH, Wong KK, Tsang OT, Chan KH, Chan JF, To KK, Chen H, Yuen KY
Journal:Nat Med
PubMed ID:32405028
A novel coronavirus-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-emerged in humans in Wuhan, China, in December 2019 and has since disseminated globally
7 total citations

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