B-27™ Supplement, minus insulin

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Gibco™

B-27™ Supplement, minus insulin

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Insulin is a customized version of theRead more

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Catalog Number	Quantity
A1895601	10 mL
A1895602	100 mL

2 Options

Catalog number A1895601

Price (USD)

188.00

Each

In stock

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Quantity:

10 mL

Price (USD)

188.00

Each

Add to cart

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Insulin is a customized version of the original B-27 Supplement without insulin. B-27 Supplement Minus Insulin is a serum-free supplement designed to support growth of hippocampal and other CNS neurons in applications where the presence of insulin causes interference. B-27 Supplement Minus Insulin is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

WARNING: Cancer – www.P65Warnings.ca.gov

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell TypeNeural Cells

Concentration50 X

Culture TypeMammalian Cell Culture

Manufacturing QualitycGMP-compliant under the ISO 13485 standard

Product LineB-27™

Quantity10 mL

Serum LevelSerum-Free

Shelf Life12 Months

Shipping ConditionDry Ice

SourceSerum-Free

Tested ForEndotoxin, Performance, Sterility, Mycoplasma

ClassificationSerum-free

FormLiquid

Product TypeCell Culture Supplement

SterilitySterile-filtered

Unit SizeEach

Contents & Storage

Store in freezer (-5 to -20°C). Protect from light.

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Frequently asked questions (FAQs)

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I thaw B-27 Supplements?

We recommend thawing it at 4 degrees C overnight or at room temperature for 2 hours. We don't recommend thawing in a water bath or incubator.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I re-freeze B-27 Supplements into smaller aliquots for future use?

Yes, we recommend that you aliquot when you first thaw it and thereafter subject the aliquot to no more than 2 additional freeze/thaw cycles.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.